* Corresponding Author
Metal Bioaccumulation in Albino Rat Tissues Treated with Decontaminated Sea Lettuce (Ulva lactuca L.)
https://doi.org/10.22146/jtbb.84533 Mulyati Mulyati(1), Susy Wijayanti(2*), Annisa Nur Islahi(3), Anindyanari Rahma Sriyekti Saraswati(4), Ashfiya Hanif Hasnadewi(5), Safira Ratri Dwi Setyasari(6)
(1) Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, 55281, Special Region of Yogyakarta, Indonesia
(2) Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, 55281, Special Region of Yogyakarta, Indonesia
(3) Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, 55281, Special Region of Yogyakarta, Indonesia
(4) Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, 55281, Special Region of Yogyakarta, Indonesia
(5) Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, 55281, Special Region of Yogyakarta, Indonesia
(6) Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, 55281, Special Region of Yogyakarta, Indonesia
(*) Corresponding Author
Abstract
Ulva lactuca is a macroalgae that contains high nutritional values. The heavy metal contaminants in natural Ulva lactuca needs to be eliminated or decreased using natural agent. The aim of this research was to determine the bioaccumulation of Pb, Cd, Hg, and the impact on liver and gastrointestinal function. Parameters of this research were Hepatosomatic Index (HSI), SGPT levels, SGOT levels, bioaccumulation Pb, Cd, Hg, and histological structure of liver and small intestine. Besides that, the progression of body weight was observed. Twelve female Wistar rats (Rattus norvegicus Berkenhout, 1769) were randomly assigned to three groups: Control, NU (treated with natural Ulva lactuca), and DU (treated with heavy metal decontaminated Ulva lactuca using Averrhoa bilimbi juice).Treatment was carried out orally at a dose of 1000 mg/Kg BW/day for 30 days. Histological structure of rat’s liver and small intestine were prepared after necropsy at the end of this research. Based on results, it can be concluded that there were no significant differences observed in HSI, SGPT, and SGOT levels among the groups. However, there was a tendency for an increase in total bilirubin levels in the decontaminated Ulva lactuca group. Both natural and heavy metal decontaminated Ulva lactuca showed histological damage on liver and small intestine. Bioaccumulation of Cd and Hg in the liver and gastrointestinal tract of rats after consuming decontaminated Ulva lactuca was lower than the natural Ulva lactuca group, but need more observations.
Keywords
Full Text:
PDFReferences
Aba, L., 2016. Bioakumulasi Merkuri pada Hepar Tikus Putih (Rattus norvegicus L.) Dengan Perlakuan Kerang Darah (Anadara Granosa L.) Tercemar Merkuri. Jurnal Penelitian Pendidikan Indonesia, 1(4), pp.33–41.
Adigüzel, Ç. & Kalender, Y., 2015. Lead nitrate induced toxic effects on small intestine tissues in diabetic and non-diabetic rats: Role of sodium selenite. Gazi University Journal of Science, 28(4), pp.541–544.
Andjelkovic, M. et al., 2019. Toxic effect of acute cadmium and lead exposure in rat blood, liver, and kidney. International Journal of Environmental Research and Public Health, 16(2), 274. doi: 10.3390/ijerph16020274.
Ardiyansyah, O., Sudarno & Rosmanida, 2019. Bioaccumulation of Cadmium (Cd) Heavy Metal on Seaweed (Gracilaria sp.) in Traditional Fishpond of Jabon Subdistrict, Sidoarjo District. IOP Conference Series: Earth and Environmental Science, 236, 012059. doi: 10.1088/1755-1315/236/1/012059.
Bais, U.E. & Lokhande, M.Y., 2012. Effect of cadmium chloride on histopathological changes in the freshwater fish Ophiocephalus striatus (Channa). International Journal of Zoological Research, 8(1), pp.23–32. doi: 10.3923/ijzr.2012.23.32.
Dardouri, K. et al., 2016. Combined Effects of Cd and Hg on Liver and Kidney Histology and Function in Wistar Rats. Journal of Agricultural Chemistry and Environment, 05(04), pp.159–169. doi: 10.4236/jacen.2016.54017.
Erlinger, S., Arias, I.M. & Dhumeaux, D., 2014. Inherited disorders of bilirubin transport and conjugation: New insights into molecular mechanisms and consequences. Gastroenterology, 146(7), pp.1625–1638. doi: 10.1053/j.gastro.2014.03.047.
Guo, J. et al., 2022. Dietary fiber intake is inversely related to serum heavy metal concentrations among US adults consuming recommended amounts of seafood: NHANES 2013–2014. Food Frontiers, 3(1), pp.142–149. doi: 10.1002/fft2.114.
Kaoud, H.A. & El-Dahshan, A.R., 2010. Bioaccumulation and histopathological alterations of the heavy metals in Oreochromis niloticus fish. Nature and Science, 8(4), pp.147–156.
Koyu, A. et al., 2006. Evaluation of the effects of cadmium on rat liver. Molecular and Cellular Biochemistry, 284(1-2), pp.81–85. doi: 10.1007/s11010-005-9017-2.
Mariadi, P.D., Kurniawan, I. & Setiawan, H., 2018. Lead and cadmium mobilization from Anas moscha and Cairina moschata tissue using pineapple extract as chelating agents. Pollution Research, 37(2), pp.330–333.
Matović, V. et al., 2011. Cadmium toxicity revisited: Focus on oxidative stress induction and interactions with zinc and magnesium. Arhiv za Higijenu Rada i Toksikologiju, 62(1), pp.65–76. doi: 10.2478/10004-1254-62-2011-2075.
Mehrandish, R., Rahimian, A. & Shahriary, A., 2019. Heavy metals detoxification: A review of herbal compounds for chelation therapy in heavy metals toxicity. Journal of HerbMed Pharmacology, 8(2), pp.69–77. doi: 10.15171/jhp.2019.12.
Motta, C.M. et al., 2022. Effects of Cadmium Exposure on Gut Villi in Danio rerio. International Journal of Molecular Sciences, 23(4), 1927. doi: 10.3390/ijms23041927.
Mulyati, Mardhatillah, T.D. & Widiyanti, S., 2020. Liver function of hypertriglyceridemia (HTG) Wistar rats (Rattus norvegicus Berkenhout, 1769) with treatments of Arthrospira maxima Setchell et Gardner and Chlorella vulgaris Beijerinck. AIP Conference Proceedings, 2260, 040013. doi: 10.1063/5.0016199
Ninkov, M. et al., 2015. Toxicity of oral cadmium intake: Impact on gut immunity. Toxicology Letters, 237(2), pp.89–99. doi: 10.1016/j.toxlet.2015.06.002.
Nurhayati, N. & Navianti, D., 2017. Pengaruh Konsentrasi Air Perasan Belimbing Wuluh Terhadap Kadar Cadmium Pada Ikan Laut. Jurnal Kesehatan Palembang, 12(1), pp.51–58.
Okon, A.J. et al., 2020. Bioaccumulation of Heavy Metals in Liver of Albino Wistar Rats Exposed to Single and Heavy Metal Mixture. African Journal of Environmental Health Sciences, 7, pp.101–109.
Qi, J. et al., 2019. Adsorption mechanism modeling using lead (Pb) sorption data on modified rice bran-insoluble fiber as universal approach to assess other metals toxicity. International Journal of Food Properties, 22(1), pp.1397–1410. doi: 10.1080/10942912.2019.1650764.
Rasyid, A., 2017. Evaluation of nutritional composition of the dried seaweed Ulva lactuca from Pameungpeuk waters, Indonesia. Tropical Life Sciences Research, 28(2), pp.119–125. doi: 10.21315/tlsr2017.28.2.9.
Sarapultsev, A.P. et al., 2012. The correctional modification of inflammatory response at the experimental acute pancreatitis. Advances in Bioscience and Biotechnology, 03(04), pp.442–448. doi: 10.4236/abb.2012.324062.
Ulfah, S., Rachmadiarti, F. & Raharjo, 2014. Upaya penurunan logam berat timbal pada Mystus nigriceps di Kali Surabaya menggunakan filtrat kulit nanas. LenteraBio, 3(1), pp.103–108.
Vigneshwar, R. et al., 2021. Sex-specific reference intervals for Wistar albino rats: hematology and clinical biochemistry. Indian Journal of Animal Health, 60(1), pp.58–65. doi: 10.36062/ijah.60.1.2021.58-65.
Wadaan, M.A.M., 2009. Effects of mercury exposure on blood chemistry and liver histopathology of male rats. Journal of Pharmacology and Toxicology, 4(3), pp.126–131. doi: 10.3923/jpt.2009.126.131.
Winiarska-Mieczan, A. & Kwiecień, M., 2016. The Effect of Exposure to Cd and Pb in the Form of a Drinking Water or Feed on the Accumulation and Distribution of These Metals in the Organs of Growing Wistar Rats. Biological Trace Element Research, 169(2), pp.230–236. doi: 10.1007/s12011-015-0414-4.
Yaich, H. et al., 2011. Chemical composition and functional properties of Ulva lactuca seaweed collected in Tunisia. Food Chemistry, 128(4), pp.895–901. doi: 10.1016/j.foodchem.2011.03.114.
Yu, Y. et al., 2021. Bioaccumulation, histopathological and apoptotic effects of waterborne cadmium in the intestine of crucian carp Carassius auratus gibelio. Aquaculture Reports, 20, 100669. doi: 10.1016/j.aqrep.2021.100669.
Yuneldi, R.F., Saraswati, T.R. & Yuniwarti, E.Y.W., 2018. Profile of SGPT and SGOT on Male Rats (Rattus norvegicus) Hyperglycemic After Giving Insulin Leaf Extract (Tithonia diversifolia). Biosaintifika, 10(3), pp.519–525.
DOI: https://doi.org/10.22146/jtbb.84533
Article Metrics
Abstract views : 665
| 
views : 304
Refbacks
- There are currently no refbacks.
Copyright (c) 2024 Journal of Tropical Biodiversity and Biotechnology
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Editoral address:
Faculty of Biology, UGM
Jl. Teknika Selatan, Sekip Utara, Yogyakarta, 55281, Indonesia
ISSN: 2540-9581 (online)