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ALPHA GLUCOSIDASE ENZYME INHIBITORY ACTIVITY TEST OF ETHYL ACETATE FRACTION OF RARU (Cotylelobium melanoxylon Pierre) LEAVES USING IN VITRO APPROACH
https://doi.org/10.22146/farmaseutik.v22i3.114547 Alif Isra Fajari(1*), Tatang irianti(2), Hari Purnomo(3)
(1) Gadjah Mada University
(2) Gadjah Mada University
(3) Gadjah Mada University
(*) Corresponding Author
Abstract
Raru (Cotylelobium melanoxylon) is a plant that has been traditionally used in North Sumatra for its potential therapeutic benefits, particularly in the treatment of diabetes. The purpose of this study was to determine the potential of the ethyl acetate fraction of Raru leaves as an antidiabetic through in vitro testing using the α-glucosidase enzyme. The research began with the method of separating compounds from methanol extract and fractionation, followed by looking at the separation of each fraction using the TLC method, glucosidase inhibition test. The results of this study indicate that the ethyl acetate fraction of Raru leaves has α-glucosidase enzyme inhibitory activity (IC50 = 7.19 ppm) which is categorized as a strong inhibitor.
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References
Bidlingmeyer, B. A. (Ed.). (1987). Preparative Liquid Chromatography (1st ed., Vol. 38). Elsevier.
Brahmachari, G. (2017). Discovery and Development of Antidiabetic Agents from Natural Products. Elsevier.
Ciesla, L., & Waksmundzka-Hajnos, M. (2011). Multidimensional and Multimodal Separations by HPTLC in Phytochemistry. In High-Performance Thin-Layer Chromatography (HPTLC) (pp. 69–92). Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-642-14025-9_5
de Torre, M. P., Vizmanos, J. L., Cavero, R. Y., & Calvo, M. I. (2024). A Novel In Vivo Method Using Caenorhabditis Elegans to Evaluate α-Glucosidase Inhibition by Natural Products for Type 2 Diabetes Treatment. Pharmaceuticals, 17(12). https://doi.org/10.3390/ph17121685
Fadimu, G. J., Farahnaky, A., Gill, H., Olalere, O. A., Gan, C. Y., & Truong, T. (2022). In-Silico Analysis and Antidiabetic Effect of α-Amylase and α-Glucosidase Inhibitory Peptides from Lupin Protein Hydrolysate: Enzyme-Peptide Interaction Study Using Molecular Docking Approach. Foods, 11(21). https://doi.org/10.3390/foods11213375
Irianti, T. T., Kuswandi, Nuranto, S., & Purwanto. (2021). Antioksidan dan Kesehatan. Gadjah Mada University Press.
Mahasuari, N. P. S., Paramita, N. L. P. V., & Putra, A. A. G. R. Y. (2020). Effect of Methanol Concentration as a Solvent an Total Phenolic and Flavonoid Content of Beluntas Leaf Extract (Pulchea indica L.). Journal of Pharmaceutical Science and Application, 2(2), 77. https://doi.org/10.24843/jpsa.2020.v02.i02.p05
Matsuda, H., Asao, Y., Nakamura, S., Hamao, M., Sugimoto, S., Hongo, M., Pongpiriyadacha, M., & Yoshikawa, M. (2009). Antidiabetogenic Constituents from the Thai Traditional Medicine Cotylelobium melanoxylon. Chem Pharm Bull, 57(5).
Mogensen, C. E. (Ed.). (2007). Pharmacotherapy of Diabetes: New Developments Improving Life and Prognosis for Diabetic Patients. Springer Science.
Mohajan, D., & Mohajan, H. K. (2024). Alpha-Glucosidase Inhibitors (AGIs): A New Class of Oral Medication for Treatment of Type 2 Diabetes Patients. Journal of Innovations in Medical Research, 3(4), 1–6. https://doi.org/10.56397/jimr/2024.12.01
Oguntibeju, O. (2014). Antioxidant-Antidiabetic Agents and Human Health. In Tech.
Pasaribu, G. (2011). Aktivitas Inhibisi Alfa Glukosidase Pada Beberapa Jenis Kulit Kayu Raru. Jurnal Penelitian Hasil Hutan, 29(1). https://doi.org/10.5
Prayoga, D. G. E., Nocianitri, A. K., & Puspawati, N. N. (2019). Identifikasi Senyawa Fitokimia dan Aktivitas Antioksidan Ekstrak Kasar Daun Pepe (Gymnema reticulatum Br.) pada Berbagai Jenis Pelarut. Jurnal Ilmu Teknologi Dan Pangan, 8(2), 111–121.
Samarakoon, D. N. A. W., Uluwaduge, D. I., & Siriwardhene, M. A. (2020). Mechanisms of Action of Sri Lankan Herbal Medicines Used in the Treatment of Diabetes: A Review. Journal of Integrative Medicine, 18(1), 14–20. https://doi.org/10.1016/j.joim.2019.11.003
Sari, M. Y., Kiswandono, A. A., Susilowati, A., Hadi, S., Yandri, Y., & Suhartati, T. (2022). Activity of α-Amylase Inhibition Against Active Compound from Raru Wood (Cotylelobium melanoxylon). Chemistry Research Journal, 7(5), 98–106. www.chemrj.org
Silalahi, M. (2019). The Phytochemistry Profile of Raru (Vatica pauciflora and Cotylelobium melanoxylon) and Its Potential as Diabetes Mellitus Drug. International Journal of Science and Research, 8(6). https://doi.org/10.21275/ART20198025
Sun, H., Saeedi, P., Karuranga, S., Pinkepank, M., Ogurtsova, K., Duncan, B. B., Stein, C., Basit, A., Chan, J. C. N., Mbanya, J. C., Pavkov, M. E., Ramachandaran, A., Wild, S. H., James, S., Herman, W. H., Zhang, P., Bommer, C., Kuo, S., Boyko, E. J., & Magliano, D. J. (2022). IDF Diabetes Atlas: Global, regional and country-level diabetes prevalence estimates for 2021 and projections for 2045. Diabetes Research and Clinical Practice, 183. https://doi.org/10.1016/j.diabres.2021.109119
Susilo, & Suciati, R. (2016). Studies Of Morphological and Secondary Metabolites Variaty of Mosses (Bryophyta) In Cibodas, West Java. International Journal of Advanced Research, 4(12), 1397–1402. https://doi.org/10.21474/IJAR01/2536
DOI: https://doi.org/10.22146/farmaseutik.v22i3.114547
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Faculty of Pharmacy
Universitas Gadjah Mada
