Ex-Vivo Study, The Effect of Standardized Eurycoma longifolia Extract on The Enzyme Activity of  Rosiglitazone N-Demethylase

Purwantiningsih Purwantiningsih; Abas Hj Hussin; Kit Lam Chan

doi:10.22146/farmaseutik.v17i1.48481

Ex-Vivo Study, The Effect of Standardized Eurycoma longifolia Extract on The Enzyme Activity of Rosiglitazone N-Demethylase

https://doi.org/10.22146/farmaseutik.v17i1.48481

Purwantiningsih Purwantiningsih^(1*), Abas Hj Hussin⁽²⁾, Kit Lam Chan⁽³⁾

(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Universiti Sains Malaysia, 11800 Penang, Malaysia
(3) School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia
(*) Corresponding Author

Abstract

Eurycoma longifolia (E. longifolia) or also known as Earth Pasak in Indonesia, has been used widely, especially to increase stamina in men. The use of herbal medicines in the long run opens up the opportunities for the influence of herbal medicines on metabolic process of other substances. This study aimed to evaluate the effect of E. longifolia extract on rosiglitazone metabolism after oral administration for one and fourteen days, and its effect on rat body weight. The rats were divided into two groups, group for one day treatment (divided into 8 subgroups with n = 6: I (control), II to VIII (were given extract at doses of 1 to 1000 mg / kg BW) and 14 days (rats divided into 4 subgroups, n = 6 : I (control), II to IV (treated with extracts of doses 5, 25 and 50 mg / kg BW). At the end of the experiment, test animals were sacrificed and rosiglitazone N-demethylase activity in hepatocytes was determined by measuring the amount of formaldehid formed at 415 nm. The rosiglitazone N-demethylase activity in all groups was analyzed by analysis of variance (ANOVA) and Tukey Test (P <0.05). The percentage of the changing in the body weight in the 14 days treatment group was compared to the control group. The results showed there was a significant increased in the rosiglitazone N-demethylase activity after rats were treated with E. longifolia extract at doses of 5 to 1000 mg / kg BB, but was not significantly different at a dose of 1 mg / kg BW when compared with control group. For the 14 days treatment, there was no significant difference between the treatment group and the control group, both for the rosiglitazone N-demethylase enzyme activity or the changed of rat body weight.

Keywords

Eurycoma longifolia, rosiglitazone N-demethylase activity, male rats

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References

Ang, H. H., Hitotsuyanagi, Y., Fukaya, H., & Takeya, K., 2002, Quassinoids from Eurycoma longifolia, Phytochemistry, 59(8), 833-837.

Ang, H., & Ngai, T. H., 2001, Aphrodisiac evaluation in non-copulator male rats after chronic administration of E. longifolia Jack, Fundamental & Clinical Pharmacology, 15, 265-268.

Ang, H. H., & Lee, K. L., 2002, Effect of Eurycoma longifolia Jack on orientation activities in middle-aged male rats, Fundamental & Clinical Pharmacology, 16, 479-483.

Ang, H. H., Lee, K. L., & Kiyoshi, M, 2003, Eurycoma longifolia Jack enhances sexual motivation in middle-aged male mice, Journal of Basic Clinical Physiology and Pharmacology, 14, 301-308.

Bent, S., & Ko, R., 2004, Commonly Used Herbal Medicines in the United States: A Review, American Journal of Medicine, 116, 478-485.

Bhat, R.; Karim, A., 2010, Tongkat Ali (Eurycoma longifolia Jack): A review on its Ethnobotany and pharmacological importance, Fitoterapia 81, 669–679.

Chan, K. L., Choo, C. Y., & Abdullah, N. R., 2005, Semisynthetic 15-O-Acyl- and 1,15-Di-O-acyleurycomanones from Eurycoma longifolia as Potential Antimalarials, Planta Medica, 71, 967-969.

Chan, K. L., Choo, C. Y., Abdullah, N. R., & Ismail, Z., 2004, Antiplasmodial studies of Eurycoma longifolia Jack using the lactate dehydrogenase assay of Plasmodium falciparum, Journal of Ethnopharmacology, 92, 223 -227.

Chan, K. L., Lee, S. P., Sam, T. W., & Han, B. H., 1989, A quassinoid glycoside from the roots of Eurycoma longifolia, Phytochemistry, 28, 2857-2859.

Chan, K. L., Lee, S. P., & Yuen, K. H., 1995, Antipyretic Activity of Quassinoids from Eurycoma longifolia Jack. In Ghazally I, Murtedza M & Laily D (Eds.), Chemical prospecting in Malayan forest, Selangor: Pelanduk Publications, 219-224.

Chandel, A., Dhindsa, S., Topiwala, S., Chaudhuri, A., & Dandona, P., 2008, Testosterone Concentrations in Young Patients With Diabetes, Diabetes Care, 31, 2013-2017.

Drewes, S. E., George, J., & Khan, F., 2003, Recent findings on natural products with erectile-dysfunction activity, Phytochemistry, 62, 1019-1025.

Hussin, A. H., & Skett, P., 1988, Lack of effect of insulin in hepatocytes isolated from streptozotocin-diabetic male rats, Biochemical Pharmacology, 37(9), 1683-1686.

Itokawa, H., Kishi, E., Morita, H., & Takeya, K., 1992, Cytotoxic quassinoids and tirucallane-type triterpene from the woods of Eurycoma longifolia, Chemical Pharmacology Bulletin, 40, 1053-1055.

Jiwajinda, S., Santisopasri, V., Murakami, A., Kawanaka, M., Kawanaka, H., Gasquet, M., Eilas, R., Balansard, G., & Ohigashi, H., 2002, In vitro anti-tumor promoting and anti-parasitic activities of the quassinoids from Eurycoma longifolia, a medicinal plant in Southeast Asia, Journal of Ethnopharmacology, 82, 55-58.

Kanchanapoom, T., Kasai, R., Chumsri, P., Hiraga, Y., & Yamasaki, K., 2001, Canthin-6-one and β-carboline alkaloids from Eurycoma harmandiana, Phytochemistry, 56, 383-386.

Kardono, L. B. S., Angerhofer, C. K., Tsauri, S., Padmawinata, K., Pezzuto, J. M., & Kinghorn, A. D., 1991, Cytotoxic and antimalarial constituents of the roots of Eurycoma longifolia, Journal of Natural Products, 54(5), 1360-1367.

Low, B. S., Ng, B. H., Choy, W. P., Yuen, K. H., & Chan, K. L., 2005, Bioavailability and pharmacokinetic studies of eurycomanone from Eurycoma longifolia, Planta Medica, 71, 803-807.

Low, W. Y., & Tan, H. M., 2007, Asian traditional medicine for erectile dysfunction, Journal of Men's Health and Gender, 4(3), 245-250.

Marbeger, M., Roehrborn, C. G., Marks, L. S., Wilson, T., & Rittmaster, R. S., 2006, Relationship among Serum Testosterone, Sexual Function, and Response to Treatment in Men Receiving Dutasteride for Benign Prostatic Hyperplasia, The Journal of Clinical Endocrinology & Metabolism, 91(4), 1323-1328.

Mills, E., Cooper, C., Seely, D., & Kanfer, I., 2005, African Herbal Medicines in the Treatment of HIV: Hypoxis and Sutherlandia. An overview of evidence and pharmacology, Nutrition Journal, 4, 19.

Morita, H., Kishi, E., Takeya, K., & Itokawa, H., 1992, Biphenylneolignans from wood of Eurycoma longifolia, Phytochemistry, 31, 3993-3995.

Nash, T., 1953, The colorimetric estimation of formaldehyde by means of the Hantzsch Reaction, Biochemical Journal, 55, 416 - 421.

Noordin, N., Othman, S. N., & Che Mat, R., 2008, Technology implementation barriers in the Malaysian herbal industry: A case study [Online]. [Accessed 10th September 2012]. Available from World Wide Web: http://www. repo.uum.edu.my/2911/1/Ruzinoor.pdf

Purwantiningsih, Hussin, A. H., & Chan, K. L., 2010, Phase I drug metabolism study of the standardised extract of Eurycoma longifolia (TAF-273) in rat hepatocytes. International Journal of Pharmacy and Pharmaceutical Sciences, 2:147-152.

Purwantiningsih, Hussin, A. H., & Chan, K. L., 2011, Herb-Drug Interaction Study of Eurycoma longifolia Extract (TAF-273) on Antidiabetic Drug (Rosiglitazone) Metabolism. Asian Journal of Pharmaceutical and Clinical Research; 4(2):88-92.

Purwantiningsih, Hussin, A. H., & Chan, K. L., 2012, Sex-Related Alterations of Aminopyrine metabolism by Standardised Extract of Eurycoma longifolia (TAF-273), Acta Alimentaria Hungaria, 41(3):316-326.

Purwantiningsih, Hussin, A. H., Chan, K. L., & Ismail S., 2014, Inhibitory Effect of Eurycoma longifolia Extract and Eurycomanone on Human Cytochrome P450 Isoforms. International Journal of Pharmacy and Pharmaceutical Sciences, 6(6):441-444.

Purwantiningsih, Hussin, A. H., & Chan, K. L., 2015, Effect of Standardized Eurycoma longifolia Extract on Rosiglitazone Metabolism in The Old Normal Male Rat Hepatocytes: A Mechanism Study. International Journal of Pharmacy and Pharmaceutical Sciences, 7(2):80-83

Salman, S. A. B., Amrah, S., Wahab, M. S. A., Ismail, Z., Ismail, R., Yuen, K. H., & Gan, S. H., 2010, Modification of propranolol’s bioavailability by Eurycoma longifolia water-based extract, Journal of Clinical Pharmacy and Therapeutics, 35(6), 691-696.

Saxena, A., Tripathi, K. P., Roy, S., Khan, F., & Sharma, A., 2008, Pharmacovigilance: Effects of herbal components on human drugs interactions involving Cytochrome P450, Bioinformation, 3(5), 198-204.

Tomar, R., Dhindsa, S., Chaudhuri, A., Mohanty, P., Garg, R., & Dandona, P., 2006, Contrasting Testosterone Concentrations in Type 1 and Type 2 Diabetes, Diabetes Care, 29(5), 1120-1122.

Wespes, E., 2002, The ageing penis, World Journal of Urology, 20, 36-39.

Zakaria, Y., Rahmat, A., Pihie, A. H. L., Abdullah, N. R., & Houghton, P. J., 2009, Eurycomanone induce apoptosis in HepG2 cells via up-regulation of p53, Cancer Cell International Journal, 9(16).

Zhang, L., Wang, Y., Zou, P., Pan, X., Zhang, H., & Chen, W., 2005, Advances in Clinical Pharmacokinetics of Herbal Medicines, US-China Medical Sciences, 2, 59-72.

DOI: https://doi.org/10.22146/farmaseutik.v17i1.48481