Efek Saponin Terhadap  Penghambatan Planktonik dan Mono-Spesies Biofilm Candida albicans ATCC 10231 pada Fase Pertengahan, Pematangan dan Degaradasi

Hasyrul Hamzah; Triana Hertiani; Sylvia Utami Tunjung Pratiwi; Titik Nuryastuti

doi:10.22146/farmaseutik.v17i2.54444

Efek Saponin Terhadap Penghambatan Planktonik dan Mono-Spesies Biofilm Candida albicans ATCC 10231 pada Fase Pertengahan, Pematangan dan Degaradasi

https://doi.org/10.22146/farmaseutik.v17i2.54444

Hasyrul Hamzah⁽¹⁾, Triana Hertiani^(2*), Sylvia Utami Tunjung Pratiwi⁽³⁾, Titik Nuryastuti⁽⁴⁾

(1) Fakultas Farmasi, Universitas Muhammadiyah Kalimantan Timur
(2) Fakultas Farmasi, Universitas Gadjah Mada
(3) Fakultas Farmasi, Universitas Gadjah Mada
(4) Fakultas Kedokteran, Kesehatan Masyarakat dan Keperawatan, Universitas Gadjah Mada
(*) Corresponding Author

Abstract

Saponin merupakan salah satu jenis metabolit sekunder dari tanaman yang memiliki aktivitas antibakteri namun aktivitas antibiofilmnya terhadap C. albicans ATCC 10231 belum pernah dilaporkan. Penemuan kandidat antibiofilm baru terhadap biofilm C. albicans menjadi tantangan yang harus di atasi dalam mencegah infeksi yang berhubungan dengan biofilm. Penelitian ini bertujuan untuk mengetahui efektivitas saponin dalam menghambat dan mendegradasi C. albicans ATCC 10231. Pengujian planktonik, penghambatan biofilm dan pengujian degradasi biofilm ditentukan dengan menggunakan metode microtiter broth. efektivitas antibiofilm saponin terhadap biofilm dianalisis dengan menghitung minimum biofilm inhibitor konsentrasi (MBIC₅₀) dan nilai minimum biofilm eradication concentration (MBEC₅₀). Senyawa saponin 1 % memberikan aktivitas penghambatan pada fase planktonik C. albicans sebesar 79,48 % ± 0,01, fase pertengahan biofilm sebesar 56,00 % ± 0,02, fase pematangan 35,55 ± 0,03 Hasilnya juga memberikan bukti aktivitas saponin dapat mendegradasi 50 % biofilm C. albicans sebesar 51,03 ± 0,01. Oleh karena itu, senyawa saponin potensial untuk dikembangkan sebagai kandidat obat-obat antibiofilm baru terhadap biofilm C. albicans.

Keywords

Saponin; Biofilm; Planktonik; C. albicans

Full Text:

PDF

References

Abbas, H.A., Serry, F.M., dan EL-Masry, E.M., 2013. Biofilms: The Microbial Castle of Resistance. Ali, I., Khan, F.G., Suri, K.A., Gupta, B.D., Satti, N.K., Dutt, P., dkk., 2010. In vitro antifungal activity of hydroxychavicol isolated from Piper betle L. Annals of Clinical Microbiology and Antimicrobials, 9: 7. Almirante, B., Rodriguez, D., Park, B.J., Cuenca-Estrella, M., Planes, A.M., Almela, M., dkk., 2005. Epidemiology and Predictors of Mortality in Cases of Candida Bloodstream Infection: Results from Population-Based Surveillance, Barcelona, Spain, from 2002 to 2003. Journal of Clinical Microbiology, 43: 1829–1835. Anderson, G.G., Palermo, J.J., Schilling, J.D., Roth, R., Heuser, J., dan Hultgren, S.J., 2003. Intracellular bacterial biofilm-like pods in urinary tract infections. Science (New York, N.Y.), 301: 105–107. Cavalheiro, M. dan Teixeira, M.C., 2018. Candida Biofilms: Threats, Challenges, and Promising Strategies. Frontiers in Medicine, 5: . Coleman, J.J., Okoli, I., Tegos, G.P., Holson, E.B., Wagner, F.F., Hamblin, M.R., dkk., 2010. Characterization of plant-derived saponin natural products against Candida albicans. ACS chemical biology, 5: 321–332. Costerton, J.W., Lewandowski, Z., Caldwell, D.E., Korber, D.R., dan Lappin-Scott, H.M., 1995. Microbial biofilms. Annual Review of Microbiology, 49: 711–745. Cowan, S.E., Gilbert, E., Liepmann, D., dan Keasling, J.D., 2000. Commensal Interactions in a Dual-Species Biofilm Exposed to Mixed Organic Compounds. Applied and Environmental Microbiology, 66: 4481–4485. Donlan, R. dan Costeron, J., 2002. Biofilm: Survival Mechanism of Clinically Relevant Microorganism.Clin Microbial Rev. Donlan, R.M., 2002. Biofilms: Microbial Life on Surfaces. Emerging Infectious Diseases, 8: 881–890. Hall-Stoodley, L., Costerton, J.W., dan Stoodley, P., 2004. Bacterial biofilms: from the Natural environment to infectious diseases. Nature Reviews Microbiology, 2: 95–108. Hamzah, H., Hertiani, T., Pratiwi, S.U.T., Murti, Y.B., dan Nuryastuti, T., 2020. The Inhibition and Degradation Activity of Demethoxycurcumin as Antibiofilm on C. albicans ATCC 10231. Research Journal of Pharmacy and Technology, 13: 377–382. Hamzah, H., Hertiani, T., Pratiwi, S.U.T., dan Nuryastuti, T., 2019. The Inhibition Activity of Tannin on the Formation of Mono-Species and Polymicrobial Biofilm Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. Majalah Obat Tradisional, 24: 110–118. Hamzah, H., Hertiani, T., Pratiwi, S.U.T., dan Nuryastuti, T., 2020. Inhibitory activity and degradation of curcumin as Anti-Biofilm Polymicrobial on Catheters. International Journal of Research in Pharmaceutical Sciences, 11: 830–835. Hamzah, H., Pratiwi, S.U.T., dan Hertiani, T., 2018. Efficacy of Thymol and Eugenol Against Polymicrobial Biofilm. Indonesian Journal of Pharmacy, 29: 214. Harriott, M.M. dan Noverr, M.C., 2010. Ability of Candida albicans Mutants To Induce Staphylococcus aureus Vancomycin Resistance during Polymicrobial Biofilm Formation. Antimicrobial Agents and Chemotherapy, 54: 3746–3755. Harriott, M.M. dan Noverr, M.C., 2011. Importance of Candida–bacterial polymicrobial biofilms in disease. Trends in Microbiology, 19: 557–563. Hertiani, T., Pratiwi, S.U.T., Irianto, I.D.K., dan Pranoto, B., 2011. Effect of Indonesian medicinal plants essential oils on Streptococcus mutans biofilm. Jin-Hyung Lee, Joo-HyoonPark, Hyun Seob Cho, Sang Woo Joo, Moo Hwan Choo, dan Jintae Lee, 2013. Anti-Biofilm activities of Quarcetin and Tannic acid againts Staphlococcus aureus,Biofoling. The Journal of Bioadhesion and Biofilm Reseac, 29:5: 491–499. Nurwijayanto, A., Na’iem, M., Wahyuono, S., dan Syahbudin, A., 2019. Screening of antioxidants properties from Understory plants of Gunung Merapi National Park (Yogyakarta, Indonesia): potential use for alternative medicine. Nuryastuti, T., Setiawati, S., Ngatidjan, N., Mustofa, M., Jumina, J., Fitriastuti, D., dkk., 2018. Antibiofilm activity of (1)-N-2-methoxybenzyl-1,10-phenanthrolinium bromide against Candida albicans. Journal de Mycologie Médicale, 28: 367–373. Pierce, C.G., Uppuluri, P., Tummala, S., dan Lopez-Ribot, J.L., 2010. A 96 well microtiter plate-based method for monitoring formation and antifungal susceptibility testing of Candida albicans biofilms. Journal of Visualized Experiments: JoVE, . Pratiwi, S.U.T. dan Hertiani, T., 2017. Efficacy Of Massoia Oil In Combination With Some Indonesian Medicinal Plants Oils As Anti-Biofilm Agent Towards Candida Albicans. International Journal of Pharmaceutical Sciences and Research, 8: 13. Pratiwi, S.U.T., Lagendijk, E.L., Hertiani, T., Weert, S.D., M, C.A., dan Hondel, J.J.V.D., 2015. Antimicrobial Effects Of Indonesian Medicinal Plants Extracts On Planktonic And Biofilm Growth Of Pseudomonas Aeruginosa And Staphylococcus Aureus. International Journal of Pharmacy and Pharmaceutical Sciences, 183–191. Wilson, C.S., 2004. 'Treatment for Recurrent Vulvovaginitis Candidiasis: An Overview of Traditional and Alternative Therapies', . DTIC Document.

DOI: https://doi.org/10.22146/farmaseutik.v17i2.54444