Penurunan Laktat Dehidrogenase Plasmodium falciparum strain D10 pada pemberian Fraksi Tithonia diversifolia (Hemsley)

Rul Afiyah Syarif; Mae Sri Hartati Wahyuningsih; Mustofa Mustofa; Ngatidjan Ngatidjan

doi:10.22146/farmaseutik.v17i1.57817

Penurunan Laktat Dehidrogenase Plasmodium falciparum strain D10 pada pemberian Fraksi Tithonia diversifolia (Hemsley)

https://doi.org/10.22146/farmaseutik.v17i1.57817

Rul Afiyah Syarif^(1*), Mae Sri Hartati Wahyuningsih⁽²⁾, Mustofa Mustofa⁽³⁾, Ngatidjan Ngatidjan⁽⁴⁾

(1) Fakultas Kedokteran Kesehatan Masyarakat dan Keperawatan (Faculty of Medicine Public Health and Nursing) Universitas Gadjah Mada
(2) Fakultas Kedokteran Kesehatan Masyarakat dan Keperawatan (Faculty of Medicine Public Health and Nursing) Universitas Gadjah Mada
(3) Fakultas Kedokteran Kesehatan Masyarakat dan Keperawatan (Faculty of Medicine Public Health and Nursing) Universitas Gadjah Mada
(4) Fakultas Kedokteran Kesehatan Masyarakat dan Keperawatan (Faculty of Medicine Public Health and Nursing) Universitas Gadjah Mada
(*) Corresponding Author

Abstract

Penelitian terdahulu menunjukkan bahwa fraksi ke-6 (F6) merupakan fraksi aktif (F-akt) daun Tithonia diversifolia (Hemsley) A.Gray yang menghambat pertumbuhan Plasmodium. Pertumbuhan parasit ini memerlukan energi yang diperoleh dari aktivitas laktat dehidrogenase (LDH). Penelitian ini bertujuan mengkaji aktivitas antiplasmodium fraksi aktif T. diversifolia terhadap kadar LDH kultur P. falciparum. Kultur Plasmodium falciparum strain D10 stadium cincin dibagi menjadi kelompok eritrosit tidak terinfeksi (KTI), eritrosit terinfeksi (KI), dan eritrosit terinfeksi Plasmodium yang diberi F6 (F-akt) T. diversifolia (KI+F-akt) konsentrasi 9,38-150 µg/mL. Kultur diinkubasi 48 jam. Media kultur diukur kadar LDH-nya secara enzimatik. Adanya perbedaan LDH antar kelompok dianalisa dengan Anova. Penghambatan aktivitas LDH (IC₅₀) ditetapkan dengan analisa probit. Kadar LDH kelompok KI (362,33 ± 133,18 U/L) lebih tinggi daripada KTI (270,33 ± 65,85 U/L) (p>0,05). Pemberian F-akt pada KI menyebabkan kadar LDH parasit lebih rendah daripada KI. Kadar LDH parasit yang diberi F-akt konsentrasi 9,38; 18,75; 37,50; dan 150 µg/mL secara berturut-turut adalah 365,5 ± 129,5; 210,5 ± 1,5; 195,5 ± 81,5; dan 111,5 ± 53,5 U/L. Tidak ada perbedaan kadar LDH antar kelompok penelitian (p>0,05). F-akt T. diversifolia mampu menghambat LDH P. falciparum strain D10 dengan nilai IC₅₀ = 39,22 µg/mL.

Kata kunci: Tithonia diversifolia, laktat dehidrogenase, Plasmodium falciparum

Keywords

Tithonia diversifolia, lactate dehydrogenase, Plasmodium falciparum

Full Text:

PDF

References

Ambre, P.K., Pissurlenkar, R.R.S., Jagyasi, A.S., ule, R.A., Khedkar, V., Barhatel, C.R., Vivas, L., Tripathi, A.K., Sullivan, D., Birkinshaw, R., Brady, L., Iyer, K., and Coutinho, E.C. 2012. Molecular Modeling Studies, Synthesis and Biological Evaluation of Novel Plasmodium falciparum Lactate Dehydrogenase (pfLDH) Inhibitors. Anti-Infective Agents 10: 55-71. https://www.researchgate.net/publication/259674306

Anonim. 2008. SYNCHRON CX® System(s) Chemistry Information Sheet: LD-P Lactate Dehydrogenase. Beckman Coulter, Inc.

Basco, L.K., Marquet, F, Makler, M.M, Le Bras, J. 1995. Plasmodium falciparum and Plasmodium vivax: lactate dehydrogenase activity and its application for in vitro drug susceptibility assay. Exp Parasitol 80(2):260-71. https://pubmed.ncbi.nlm.nih.gov/7895836/

Cobben N. A. M., Drent, M., Schols, A. M. W. J., Lamers R. J. S., Wouters E. F. M., and Van Dieijen-Visser, M. P.1997. Serum lactate dehydrogenase and its isoenzyme pattern in ex-coalminers. Respiratory Medicine 91: 616-623. https://www.mendeley.com/catalogue/d7eb36da-5dff-37be-bf66-1bfe2eb8ab19/

Contreras, C.E., Rivas, M.A., Dominguez, J., Charris, J., Palacios, M., Bianco, N.E., and Blanca, I. 2004. Stage-specifics Activity of Potential Antimalarial Compounds Measured in vitro by Flow Cytometry in Comparison to Optical Microscopy and Hypoxanthine Uptake. Mem Inst Cruz. 99(2):179-84. https://pubmed.ncbi.nlm.nih.gov/15250472/

Garba, I.H. dan Ubom, G.A.2005. Total serum lactate dehydrogenase activity in acute Plasmodium falciparum malaria infection. Singapore Med J 46(11):632-634

Grace, N.N., Rainer, B.W., Barbara, G, Erie, N.L., and Victoris, N. 2004. Utilization of Weed Species as Sources of Traditional Medicines in Central Kenya. Lyonia., 7(2) : 71-87.

Kalra B.S., Chawla S., Gupta P., and Valecha N. 2006. Screening of antimalarial drugs: An overview. Indian J Pharmacol 38 (1): 5-12

Pradhan, A, Tripathi, A.K., Desai, P.V., Mukherjee, P.K., Avery, M.A., Walker, L.A., Tekwani, B.L. 2009. Structure and function of Plasmodium falciparum malate dehydrogenase: Role of critical amino acids in co-substrate binding pocket. Biochimie 91: 1509–1517. http://dx.doi.org/10.1016/j.biochi.2009.09.005

Syarif RA, Wahyuningsih MSH, Mustofa, Ngatidjan, Kurniawan H, Al Hilal SR. 2008. Aktivitas antiplasmodium in vitro ekstrak kembang bulan (Tithonia diversifolia (Hemsley) A.Gray) terhadap Plasmodium falciparum. Majalah Obat Tradisional 13 (43): 3- 9.

Syarif, R.A., Wahyuningsih, M.S.H, Mustofa, Ngatidjan. 2018. Antiplasmodial activity and onset speed of growth inhibition of Tithonia diversifolia (Hemsley) A.Gray leave fractions on Plasmodium falciparum. Trop. J. Pharm 17 (11): 2213-2218. https://www.tjpr.org/admin/12389900798187/2018_17_11_15.pdf

Valvona, C.J., Fillmore, H.L., Nunn, P.B., Pilkington, G.J. 2016. REVIEW The Regulation and Function of Lactate Dehydrogenase A: Therapeutic Potential in Brain Tumor. Brain Pathology 26: 3-17

Verma, P., Biswas, S., Mohan, T., Ali, S., & Rao, D.N. 2013. Detection of histidine rich protein & lactate dehydrogenase of Plasmodium falciparum in malaria patients by sandwich ELISA using in-house reagents. Indian J Med Res 138: 977-987. https://pubmed.ncbi.nlm.nih.gov/24521645/

Vivas, L., Easton, A., Kendrick, H., Cameron, A., Lavandera, J.L., Barros, D., de las Heras, F.G., Brady, R.L., and Croft, S,L. 2005. Plasmodium falciparum: stage specific effects of a selective inhibitor of lactate dehydrogenase. Exp Parasitol 111(2):105-14. https://pubmed.ncbi.nlm.nih.gov/16098967/

DOI: https://doi.org/10.22146/farmaseutik.v17i1.57817