Skrining Jamur Tanah Penghasil L-asparaginase dan Aktivitas Antibakterinya Terhadap Escherichia coli dan Staphylococcus aureus

Indah Kurniawati; Purwanto Purwanto; Indah Purwantini

doi:10.22146/farmaseutik.v19i2.82558

Skrining Jamur Tanah Penghasil L-asparaginase dan Aktivitas Antibakterinya Terhadap Escherichia coli dan Staphylococcus aureus

https://doi.org/10.22146/farmaseutik.v19i2.82558

Indah Kurniawati⁽¹⁾, Purwanto Purwanto⁽²⁾, Indah Purwantini^(3*)

(1) Magister Ilmu Farmasi, Fakultas Farmasi, Universitas Gadjah Mada
(2) Faculty of Pharmacy, Universitas Gadjah Mada, Indonesia
(3) Faculty of Pharmacy, Universitas Gadjah Mada, Indonesia
(*) Corresponding Author

Abstract

Indonesia merupakan salah satu negara beriklim tropis yang memiliki kelembaban tinggi sehingga memungkinkan untuk tumbuhnya berbagai tanaman dan mikroorganisme yang baik. Salah satu mikroorganisme yang dapat tumbuh dengan baik di Indonesia adalah jamur. Mikroorganisme ini merupakan produsen yang sangat baik dari enzim hidrolitik, biofuel, asam organik, polisakarida, dan metabolit sekunder yang dapat dikembangkan sebagai obat antibiotik, obat antikanker, agen hipokolesterolemia, imunosupresan, dan lain-lain. Sebanyak 12 jamur berhasil diisolasi dari tanah di Bantul, Merapi dan Gunung kidul. Hasil uji menunjukkan bahwa 7 isolat jamur menghasilkan enzim L-asparaginase yaitu PB1, PB2, PRB1, PB4, PB3, PRGK2 dan PGK1, sedangkan 4 isolat jamur menunjukkan aktivitas antibakteri dengan zona hambat terbesar adalah isolat jamur CM sebesar 4,649 mm terhadap bakteri Staphylococcus aureus dan sebesar 13,681 mm terhadap bakteri Escherichia coli.

Keywords

jamur; tanah; L-asparaginase; antibakteri

Full Text:

PDF

References

Baghdadi, A. M., & Balobaid, A. K. (2021). Isolation and Molecular Identification of Fungi Producing of L-asparaginase Isolated from Makkah Region Soil. World Journal of Environmental Biosciences, 10(2), 42–48. https://doi.org/10.51847/HuDTuS0bhw

Balasubramanian, A. (2017). Characteristics of Soil Profile. Centre for Advanced Studies in Earth Science. University of Mysore, Mysore.

Batool, T., Makky, E. A., Jalal, M., & Yusoff, M. M. (2016). A Comprehensive Review on l-Asparaginase and Its Applications. Applied Biochemistry and Biotechnology, 178(5), 900–923. https://doi.org/10.1007/s12010-015-1917-3

Fajeriyati, N. (2017). (Antibacterial Activity of Ethanol Extract of Kencur Rhizome (Kaempferia galanga L.) in Bacillus subtilis and Escherichia coli). Journal of Current Pharmaceutical Sciences. 1(1).

Fanida, Z. M., & Ardiningsih, P. (2019). Isolasi Dan Uji Aktivitas Antibakteri Jamur (Fungi) Tanah Gambut Pontianak. Jurnal Kimia Khatulistiwa. 7.

Freitas, M., Souza, P., Cardoso, S., Cruvinel, K., Abrunhosa, L. S., Ferreira Filho, E. X., Inácio, J., Pinho, D. B., Pessoa, A., & O. Magalhães, P. (2021). Filamentous Fungi Producing l-Asparaginase with Low Glutaminase Activity Isolated from Brazilian Savanna Soil. Pharmaceutics, 13(8), 1268. https://doi.org/10.3390/pharmaceutics13081268

Hasanin, M. S. (2020). Eco-friendly, economic fungal universal medium from watermelon peel waste. Journal of Microbiological Methods, 9.

Lestari, E. S., Severin, J. A., Filius, P. M. G., Kuntaman, K., Duerink, D. O., Hadi, U., Wahjono, H., Verbrugh, H. A., & On behalf of the study group Antimicrobial Resistance in Indonesia: Prevalence and Prevention (AMRIN). (2007). Antimicrobial resistance among commensal isolates of Escherichia coli and Staphylococcus aureus in the Indonesian population inside and outside hospitals. European Journal of Clinical Microbiology & Infectious Diseases, 27(1), 45–51. https://doi.org/10.1007/s10096-007-0396-z

Marie A & Chisholm-Burns. (2019). Pharmacotherapy Principles and Practice, Fifth Edition. http://www.vlebooks.com/vleweb/product/openreader?id=none&isbn=9781260019452

Priya, S. R., & Subhashini, A. (2022). Characterization and Optimization of Fungal L-Asparaginase Isolated From Soil and Medicinal Plants. Journal of Pure and Applied Microbiology, 16(1), 453–459. https://doi.org/10.22207/JPAM.16.1.43

Rani, S. A., Sundaram, L., & Vasantha, P. B. (2012). isolation and screening of l-asparaginase producing fungi from soil samples. International Journal of Pharmacy and Pharmaceutical Sciences. 4(1), 4.

Rosyadi, A., Triatmoko, B., & Nugraha, A. S. (2022). Isolation of Estuary Soil Fungi and Screening Antibacterial Activity Against Staphylococcus aureus. Indonesian Journal of Pharmaceutical Science and Technology, 9(1), 16. https://doi.org/10.24198/ijpst.v1i1.28834

Susanti, M. A., Mahardhika, G. S., Rujito, L., Darmawan, A. B., & Anjarwati, D. U. (2020). The Examination of mecA gene in Methicillin-Resistant Staphylococcus aureus (MRSA) and inappropriate antibiotic uses of healthcare workers and communities in Banyumas. Jurnal Kedokteran Dan Kesehatan Indonesia, 11(3), 257–265. https://doi.org/10.20885/JKKI.Vol11.Iss3.art7

Wibowo, M. S., Julianti, E., Radzali, M. D. (2017). Isolation and Antibacterial Activity of Soil-Derived Fungi From Taman Botani Negara, Shah Alam, Malaysia. Acta Pharmaceutica Indonesia, 42(1), 18-24.

DOI: https://doi.org/10.22146/farmaseutik.v19i2.82558