The use of carbonate apatite bioglass cement as pulp capping material examined from reparative dentin

https://doi.org/10.22146/majkedgiind.43469

Indah Puti Rahmayani Sabirin(1*), Myrna Nurlatifah Zakaria(2)

(1) Department of Oral Biology, Program Study of Dentistry, Faculty of Medicine, Universitas Jenderal Achmad Yani, West Java
(2) Department of Endodontology and Operative Dentistry, Program Study of Dentistry, Faculty of Medicine, Universitas Jenderal Achmad Yani, West Java
(*) Corresponding Author

Abstract


Root canal infection potentially develops to periapical infection as a result of opening of pulp chamber as a pathway for irritants to periapical area. Proper pulp capping procedure can be done to prevent root canal infection by preserving the vitality of the pulp and inducing reparative dentin. Generally, calcium hydroxide (Ca(OH2)) is used as pulp capping material although it stil3Ap) and CO3Ap bioglass cement are bioceramic materials proven for their ability to form new bone structure upon a research on bone tissue. These materials have better mechanical strength than Ca(OH2) and have the ability to set in humid environment similar to the body’s physiological condition. This study was a preclinical trial of CO3Ap and CO3Ap bioglass cement as pulp capping material. The materials were applied on a perforation site of 24 Wistar rat teeth, divided into 4 groups of different treatments, negative control (no treatment), positive control (Ca(OH2)), CO3Ap, and CO3Ap bioglass, respectively. Following the application of the pulp capping materials, the perforation sites were covered with light-cured glass ionomer cement. Evaluation of reparative dentin was performed after 21 days, where the rats were euthanized, and histopathological specimens were stained using hematoxylin and eosin. The results of the study revealed that dentinal bridge was formed on all the treatment and positive control groups (p=0.000) but not on the negative control. We concluded that CO3Ap as well as CO3Ap bioglass cement have the ability to induce the formation of reparative dentin, therefore both materials can be considered as pulp capping material for open pulp or thin dentin condition on pulp chamber roof.

Keywords


bioceramic cement; bioglass, carbonate apatite; dentinal bridge; pulp capping

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References

1. Hilton TJ. Key to clinical success with pulp capping: a review of the literature. Oper Dent. 2009; 34(5): 615–625. doi: 10.2341/09-132-0

2. Fouad AF, Levin L. Pulpa reactions to caries and dental procedures. In: Hargreaves KM, Cohen S, Berman LH, editor. Cohen’s pathways of the pulp. 10th ed. St Louis: Elsevier Mosby; 2011. 463-480, 506-509.

3. Huang GTJ. Pulp and dentin tissue engineering and regeneration: current progress. Regen Med. 2009; 4(5): 697-707. doi: 10.2217/rme.09.45

4. Best SM, Porter AE, Thian ES, Huang J. Bioceramics: past, present and for the future. J Eur Ceram Soc. 2008; 28(7): 1319-1327. doi: 10.1016/j.jeurceramsoc.2007.12.001

5. Nasseh AA. The rise of bioceramics. Endodontic Practice. 2009; 2: 17-22.

6. Ishikawa K. Calcium phosphate cements. In Bioceramics and Their Clinical Application. Elsevier Inc; 2008. 438-463.

7. Ishikawa K. Bone substitute fabrication based on dissolution-precipitation reactions. Materials (Basel). 2010; 3(2): 1138–1155. doi: 10.3390/ma3021138

8. Cahyanto A, Maruta M, Tsuru K, Matsuya S, Ishikawa K. Fabrication of bone cement that fully transform to carbonate apatite. Dent Mater J. 2015; 34(3): 394-401. doi: 10.4012/dmj.2014-328

9. Al-Sabbagh M, Burt J, Barakat A, Kutkut A, El-Ghannam A. Alveolar ridge preservation using resorbable bioactive ceramic composite: a histological study. J Int Acad Periodontol. 2013; 15(3): 91-98.

10. Aniket YA, Marriott I, El-Ghannam A. Promotion of pro-osteogenic responses by
a bioactive ceramic coating. J Biomed Mater Res A. 2012; 100(12): 3314–3325.
doi: 10.1002/jbm.a.34280

11. Zakaria MN, Pauziah NFN, Sabirin IP, Cahyanto A. Evaluation of carbonate apatite in inducing formation of reparative dentin in exposed dental pulp. Key Eng Mater. 2017; 758: 250–254. doi: 10.4028/www.scientific.net/KEM.758.250

12. Zakaria MN, Cahyanto A, El-Ghannam. Calcium release and physical properties of modified carbonate apatite cement as pulp capping agent in dental application. Biomater Res. 2018; 22: 35.

13. Zakaria MN, Cahyanto A, El Ghannam A. Basic properties of novel bioactive cement based in silica-calcium phosphate composite and carbonate apatit. Key Eng Mater. 2016; 720: 147-152. doi: 10.4028/www.scientific.net/KEM.720.147

14. Cahyanto A, Restunaesha M, Zakaria MN, Rezano A, El-Ghannam A. Compressive strength evaluation and phase analysis of pulp capping materials based on carbonate apatite-SCPC using different concentration of SCPC and calcium hydroxide. Key Eng Mater. 2018; 782: 15–20. doi: 10.4028/www.scientific.net/KEM.782.15

15. Rosner B. Fundamentals of Biostatistics. 6th ed. Belmont: Duxbury; 2005.

16. Dammaschke T, Stratmann U, Fischer RJ, Sagheri D, Schäfer E. Proliferation of rat molar pulp cells after direct pulp capping with dentine adhesive and calcium hydroxide. Clin Oral Investig. 2011; 15(4): 577–587. doi: 10.1007/s00784-010-0409-7

17. Suzuki M, Taira Y, Kato C, Shinkai K, Katoh Y. Histological evaluation of direct pulp capping of rat pulp with experimentally developed low-viscosity adhesives containing reparative dentin-promoting agents. J Dent. 2016; 44: 27–36. doi: 10.1016/j.jdent.2015.11.005

18. Louwakul P, Lertchirakarn V. Response of Inflamed Pulps of Rat Molars after Capping with Pulp-capping Material Containing Fluocinolone Acetonide. J Endod. 2015; 41(4): 508-512. doi: 10.1016/j.joen.2014.12.004

19. Parolia A, Kundabala M, Rao NN, Acharya SR, Agrawal P, Mohan M, Thomas M. A comparative histological analysis of human pulp following direct pulp capping with propolis, mineral trioxide aggregate and dycal. Aust Dent J. 2010; 55(1): 59-64. doi: 10.1111/j.1834-7819.2009.01179.x

20. Teti G, Salvatore V, Ruggeri A, Manzoli L, Gesi M, Orsini G, Falconi M. In vitro reparative dentin: a biochemical and morphological study. Eur J Histochem. 2013; 57(3): e23. doi: 10.4081/ejh.2013.e23

21. Song M, Yu B, Kim S, Hayashi M, Smith C, Sohn S. Clinical and molecular perspectives of reparative dentin formation: Lessons learned from pulp-capping materials and the emerging roles of calcium. Dent Clin North Am. 2017;
61(1): 93–110. doi: 10.1016/j.cden.2016.08.008

22. Zakaria MN. Saving the pulp and essential issues in pulp capping treatment. J Dentomaxillofac Sci. 2016; 1(2): 73-76.



DOI: https://doi.org/10.22146/majkedgiind.43469

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