* Corresponding Author
Korelasi Jumlah Streptococcus mutans (S. mutans) dan Level Ekspresi Interlukin 8 (IL-8) pada Severe Early Childhood Caries
https://doi.org/10.22146/majkedgiind.9227 Muhammad Luthfi(1*), Retno Indrawati(2), Ira Arundina(3), Yoes Prijatna Dachlan(4)
(1) Departemen Biologi Oral, Fakultas Kedokteran Gigi, Universitas Airlangga, Surabaya, Jawa Timur, Indonesia
(2) Departemen Biologi Oral, Fakultas Kedokteran Gigi, Universitas Airlangga, Surabaya, Jawa Timur, Indonesia
(3) Departemen Biologi Oral, Fakultas Kedokteran Gigi, Universitas Airlangga, Surabaya, Jawa Timur, Indonesia
(4) Departemen Parasitologi, Fakultas Kedokteran, Universitas Airlangga, Surabaya, Jawa Timur, Indonesia
(*) Corresponding Author
Abstract
Correlation of Streptococcus mutans (S. mutans) Level and Interleukin 8 (IL-8) Expressions of Salivary Neutrophils in Severe Early Childhood Caries. Early childhood caries is a very serious health problem because it is a chronic infectious disease that is contagious. Dental caries begins after the primary teeth grow and develop on the tooth surface very quickly and progressively. In recent years the views of neutrophils have changed dramatically. Neutrophils not only act as a microbe killer through phagocytosis, the release of reactive oxigen species (ROS) and its antimicrobial peptide, but neutrophil activation also helps regulate the immune response. To analyze the relationship between the amount S. mutans and IL-8 expression of salivary neutrophils in severe early Childhood caries. Two groups, namely Isolation of S. mutans were performed on saliva samples taken from 20 caries-free and 20 severe early childhood caries and samples Nacl 1,5% mouthwash results of 20 caries-free and 20 severe early childhood caries salivary neutrophils that were analysis of IL-8 expression by flow cytometry. Based on the average value, it is known that S. mutans level in early Childhood caries-free is lower (513.500,00 +185.565,28 CFU/ml) in comparison to the severe early Childhood caries (977.000,00 +222.500,15 CFU/ml), but the expression of IL-8 neutrophil salivary neutrophils in early Childhood caries-free is higher (3,31+0,50) in comparison to the severe early Childhood caries (2,95+0,56). The increased S. mutans level is probably caused by the decrease in the expression of IL-8 salivary neutrophils in severe early childhood caries.
Keywords
Full Text:
PDFReferences
American Academy of Pediatric Dentistry, Organizing Council. Definition of early chilhood caries (ECC). Pediatr Dent. 2003; 25: 9.
Filstrups SL, Briskie D, Fonseca M, Lawrence L, Wandera A, Inglehart MR. ECC and quality of life: child and parent perspectives. Pediatr Dent. 2003; 25: 431-440.
Tinanoff N, Reisine S. Update on early childhood caries since the Surgeon General’s Report. Academic Pediatr. 2009; 9(6): 396-403.
Fitriani. Faktor resiko karies gigi sulung anak (studi kasus anak TK Pangeran Diponegoro Semarang). Skripsi. Semarang: Universitas Diponegoro; 2007.
Indrawati R. Aktivitas enzim dextranase dan sebaran genotype Streptococcus mutans penderita karies dan bebas karies. Disertasi.
Surabaya. Program Pascasarjana Universitas Airlangga; 2006.
Takahashi N, Horiuchi M, Yamada T. Effects of acidification on growth and glycolysis of streptococcus sanguis and streptococcus
mutans. Oral Microbiol Immunol. 1997; 12(2): 72-6.
Elbim C, Katsikis PD, Estaquier J. Neutrophil apoptosis during viral infections. Open Virol J. 2009; 3:52-9.
Nathan. Neutrophils and immunity: challenges and opportunities. Nat Rev Immunol. 2006; 3:173-82.
Mantovani AMA, Cassatella C, Costantini, Jaillon S. Neutrophils in the activation and regulation of innate and adaptive immunity.
Nat Rev Immunol. 2011; 11: 519-531.
Baggiolini M, Dewald B, Moser B. Human chemokines: An update. Annu Rev Immunol. 1997; 15: 675–705.
Kobayashi SD, Voyich JM, Burlak C, DeLeo FR. Neutrophils in the innate immune response. Arch Immunol Ther Exp. 2005; 53:
-517.
Phattarataratip E. The role of salivary antimicrobial peptides in shaping Streptococcus mutans ecology. Dissertation, University of Iowa disease pathogenesis. Annu Rev Microbiol. 2010; 57:677-701.
Gasparoto TH, Vieira NA, Porto VC, Campanelli AP, Lara VS. Differences between salivary and blood neutrophils from elderly
and young denture wearers. J Oral Rehabil. 2011; 38(1): 41-51.
Borjesson DL, Kobayashi SD, Whitney AR, Voyich JM, Argue CM, DeLeo FR. Insights into pathogen immune evasion mechanisms: Anaplasma phagocytophilum fails to induce an apoptosis differentiation program in human neutrophils. J Immunol. 2005; 174: 6364–6372.
Tanzer JM, Livingston J, Thompson AM. The microbiology of primary dental caries in humans. J Dental Edu. 2001; 65(10): 1028-1037.
Tinanoff N, Reisine S. Update on early childhood caries since the Surgeon General’s Report. Academic Pediatr. 2009; 9(6): 396-
Akira S, Takeda K. Toll-like receptor signalling. Nat Rev Immunol. 2004; 4: 499–511.
Sorensen OE, Follin P, Johnsen AH, Calafat J, Tjabringa GS, Hiemstra PS, Borregaard N. Human cathelicidin, hCAP-18, is processed to the antimicrobial peptide LL-37 by extracellular cleavage with proteinase 3. Blood. 2001; 4:421- 424.
Brasier AR, Jamaluddin M, Casola A, Duan W, Shen Q, Garofalo RP. J Biol Chem. 1998; 273, 3551–3561.
Juffrie M, van der Meer GM, Hack CE, Haasnoot K, Sutaryo, Meerman AJP, Thijs LG. Inflammatory mediators in dengue virus infection in children: interleukin-8 and its relationship to neutrophil degranulation. Infect Immun. 2000; 68:702–707.
Kim JS, Kim JM, Jung HC, Song IS. Expression of cyclooxygenase-2 in human neutrophils activated by Helicobacter pylori water-soluble proteins. Dig Dis Sci. 2001; 46: 2277–2284.
Ryun JS, Kang JH, Jung SY, Shin MH, Kim JM, Park H, Min DY. Production of interleukin-8 by human neutrophils stimulated with
trichomonas vaginalis. Infect Immun. 2004; 72(3): 1326–1332.
DOI: https://doi.org/10.22146/majkedgiind.9227
Article Metrics
Abstract views : 4586
| 
views : 12199
Refbacks
- There are currently no refbacks.
Copyright (c) 2016 Majalah Kedokteran Gigi Indonesia