* Corresponding Author
Combining antimicrobial photodynamic therapy with curcumin and with methylene blue against S. mutans
https://doi.org/10.22146/majkedgiind.94888 Ninuk Hariyani(1*), Dini Setyowati(2), Talsha Irzaputri Armadanty(3), Devy Ratriana Amiati(4), Anugrah Putra Dani(5), Lily Azura Shoaib(6), Namita Shanbhag(7)
(1) Department of Dental Public Health, Faculty of Dental Medicine, Universitas Airlangga
(2) Department of Dental Public Health, Faculty of Dental Medicine, Universitas Airlangga
(3) Post-Graduate Program in Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Indonesia
(4) Post-Graduate Program in Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Indonesia Faculty of Dentistry, Bhakti Wiyata Institute of health sciences, Kediri, Indonesia
(5) Department of Dental Public Health, Faculty of Dental Medicine, Universitas Airlangga, Indonesia
(6) Faculty of Dentistry, University of Malaya, Malaysia.
(7) Government dental college & research institute, Bangalore, India
(*) Corresponding Author
Abstract
ntimicrobial photodynamic treatment (aPDT) is a treatment method that links a light source to a photosensitizing carrier. There is no clear standardization regarding the exact therapeutic concentration when it is combined with curcumin or methylene blue, and there is no comparison of their activities toward S. mutans. We aimed to assess the best composition of the combination of aPDT and curcumin and with the combination of aPDT and methylene blue in reducing the number of S. mutans biofilms. The searches were carried out in Pubmed, ScienceDirect, and Proquest from 2015 to April 2023; collecting in-vitro and in-vivo research. Two authors independently reviewed studies, assessed their quality and eligibility, then extracted the data. 14 articles that met the inclusion criteria were retained. The final results were then grouped into 2, showing the effectiveness for a combination of aPDT and methylene blue and of aPDT and cucumin (9 and 5 articles, respectively). Each combination has different aPDT properties. The combination of aPDT and methylene blue is effective in reducing S. mutant biofilm in 660 nm diode laser wavelength and 40 mW output power. Furthermore, aPDT combined with curcumin is effective in reducing S. mutants biofilm in a wavelength of 405 nm and an irradiation time of 50-150 seconds. The addition of EDTA in a combination of aPDT and curcumin could increase its effectivity.
Keywords
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12. Ninuk HaryaniReferences
1. Scharnow AM, Solinski AE, Wuest WM. Targeting S. mutans biofilms: a perspective
on preventing dental caries. MedChemComm. 2019; 10(7): 1057–1067.
doi: 10.1039/C9MD00015A
2. Zhang Y, Fang J, Yang J, Gao X, Dong L, Zheng X, Sun L, Xia B, Zhao N, Ma Z, Wang Y. Streptococcus mutans-associated bacteria in dental plaque of severe early childhood caries. J Oral Microbiol. 2022; 14(1): 2046309. doi: 10.1080/20002297.2022.2046309
3. Marsh PD, Lewis MAO, Rogers H, Williams D, Wilson M. Oral Microbiology. 6th ed.
Philadelphia: Elsevier Health Sciences; 2016.
4. Hamblin MR, Huang Y. Imaging in photodynamic Therapy. 1st ed. Boca Raton: CRCPress; 2017.
5. Zhao A, Sun J, Liu Y. Understanding bacterial biofilms: From definition to treatment
strategies. Front Cell Infect Microbiol. 2023; 13: 1137947. doi: 10.3389/fcimb.2023.1137947
6. Lemos JA, Palmer SR, Zeng L, Wen ZT,Kajfasz JK, Freires IA, Abranches J, Brady LJ. The biology of Streptococcus mutans. Microbiol Spectr. 2019; 7(1): 1-18.
doi: 10.1128/microbiolspec.GPP3-0051-2018
7. Jakubovics NS, Goodman SD, Mashburn-Warren L, Stafford GP, Cieplik F. The dental
plaque biofilm matrix. Periodontol 2000. 2021; 86(1): 32-56.
doi: 10.1111/prd.12361. Epub 2021 Mar 10
8. Warreth A. Dental caries and its management. International Journal of Dentistry. 2023; 2023:
1–15. doi: 10.1155/2023/9365845
9. Barazy R, Alafif H, Achour H, Al-Aloul A, Alsayed Tolibah Y. Can antimicrobial photodynamic therapy serve as an effective adjunct protocol for disinfecting the necrotic root canal system? A randomized controlled study. BDJ Open. 2024; 10(1): 53.
doi: 10.1038/s41405-024-00239-y
10. Memar MY, Ghotaslou R, Samiei M, Adibkia K. Antimicrobial use of reactive oxygen
therapy: current insights. Infection and Drug Resistance. 2018; 11: 567–576.
doi: 10.1038/s41405-024-00239-y
11. Convissar RA. Principles and Practice of Laser Dentistry. 3rd ed. Missouri: Elsevier
Health Sciences; 2022.
12. Ramalho KM, Rocha RG, Correa-Aranha AC, Cunha SR de B, Simões A, Campos L,
et al. Treatment of herpes simplex labialis in macule and vesicle phases with photodynamic
therapy. Report of two cases. Photodiagnosis and Photodynamic Therapy. 2015; 12(2):
321–323. doi: 10.1016/j.pdpdt.2015.02.005
13. Simões A, Benites BM, Benassi C, Torres-Schroter G, de Castro JR, Campos L. Antimicrobial photodynamic therapy on treatment of infected radiation-induced oral mucositis: Report of two cases. Photodiagnosis and Photodynamic Therapy. 2017; 20: 18–20.
doi: 10.1016/j.pdpdt.2017.08.007
14. Tahmasebi E, Keshvad A, Alam M, Abbasi K, Rahimi S, Nouri F, et al. Current Infections
of the orofacial region: treatment, diagnosis, and epidemiology. Life. 2023; 13(2): 269. doi: 10.3390/life13020269
15. Cieplik F, Buchalla W, Hellwig E, Al-Ahmad A, Hiller KA, Maisch T, et al. Antimicrobial
photodynamic therapy as an adjunct for treatment of deep carious lesions - A
systematic review. Photodiagnosis and Photodynamic Therapy. 2017; 18: 54–62.
doi: 10.1016/j.pdpdt.2017.01.005
16. Boltes Cecatto R, Siqueira de Magalhães L, Fernanda Setúbal Destro Rodrigues M, Pavani C, Lino-dos-Santos-Franco A, Teixeira Gomes M, et al. Methylene blue mediated antimicrobial photodynamic therapy in clinical human studies: The state of the art. Photodiagnosis and Photodynamic Therapy. 2020; 31: 101828. doi: 10.1016/j.pdpdt.2020.101828
17. Manolova Y, Deneva V, Antonov L, DrakalskaE, Momekova D, Lambov N. The effect of
the water on the curcumin tautomerism: A quantitative approach. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2014; 132: 815–820.
doi: 10.1016/j.saa.2014.05.096
18. Schiborr C, Kocher A, Behnam D, Jandasek J, Toelstede S, Frank J. The oral bioavailability
of curcumin from micronized powder and liquid micelles is significantly increased in healthy
humans and differs between sexes. Molecular Nutrition & Food Research. 2014; 58(3): 516–
527. doi: 10.1002/mnfr.201300724
19. Diniz IMA, Horta ID, Azevedo CS, Elmadjian TR, Matos AB, Simionato MRL, et al.
Antimicrobial photodynamic therapy: A promise candidate for caries lesions treatment. Photodiagnosis and Photodynamic Therapy. 2015; 12(3): 511–518.
doi: 10.1016/j.pdpdt.2015.04.006
20. Nemezio MA, de Souza Farias SS, Borsatto MC, Aires CP, Corona SAM. Effect of
methylene blue-induced photodynamic therapy on a Streptococcus mutans biofilm
model. Photodiagnosis and Photodynamic Therapy. 2017; 20: 234–237.
doi: 10.1016/j.pdpdt.2017.10.025
21. Neves PAM, Lima LA, Rodrigues FCN, Leitāo TJ, Ribeiro CCC. Clinical effect of
photodynamic therapy on primary carious dentin after partial caries removal. Braz Oral
Res. 2016; 30(1). doi: 10.1590/1807-3107BOR-2016.vol30.0047
22. Mocuta (Bojoga) DE, Miron M, Hogea E, Muntean C, Todea D. Dental-Plaque
Decontamination around Dental Brackets Using Antimicrobial Photodynamic Therapy:
An In Vitro Study. International Journal of Environmental Research and Public Health.
2021; 18(23): 12847. doi: 10.3390/ijerph182312847
23. Furtado GS, Paschoal MAB, Santos Grenho L do C, Lago ADN. Does pre-irradiation
time influence the efficacy of antimicrobial photodynamic therapy? Photodiagnosis and
Photodynamic Therapy. 2020; 31: 101884. doi: 10.1016/j.pdpdt.2020.101884
24. Méndez DAC, Gutierrez E, Dionísio EJ, Oliveira TM, Buzalaf MAR, Rios D, et al. Effect
of methylene blue-mediated antimicrobial photodynamic therapy on dentin caries
microcosms. Lasers in Medical Science. 2018; 33(3): 479–487.
doi: 10.1007/s10103-017-2379-3
25. Azizi A, Mousavian S, Taheri S, Lawaf S, Gonoudi E, Rahimi A. Comparison of the
antimicrobial efficacy of photodynamic therapy with two mediators against Lactobacillus
acidophilus in vitro. Photodiagnosis and Photodynamic Therapy. 2018; 21: 357–362.
doi: 10.1016/j.pdpdt.2018.01.014
26. Pereira LM, Estevam LR, da Silva MF, Pinheiro SL. Polyacrylic acid with methylene blue dye as a sensitizing agent for photodynamic therapy to reduce streptococcus mutans in dentinal
caries. Photobiomodulation, Photomedicine, and Laser Surgery. 2020; 38(11): 687–693.
doi: 10.1089/photob.2019.4736
27. Alhenaki AM, Alqarawi FK, Tanveer SA, Alshahrani FA, Alshahrani A, AlHamdan EM,
et al. Disinfection of acrylic denture resin polymer with Rose Bengal, Methylene blue
and Porphyrin derivative in photodynamic therapy. Photodiagnosis and Photodynamic Therapy. 2021; 35: 102362. doi: 10.1016/j.pdpdt.2021.102362.
28. Hwang HR, Lee ES, Kang SM, Chung KH, Kim BI. Effect of antimicrobial photodynamic
therapy with Chlorella and Curcuma extract on Streptococcus mutans biofilms. Photodiagnosis and Photodynamic Therapy. 2021; 35: 102411. doi: 10.1016/j.pdpdt.2021.102411.
29. Lamarque GCC, Méndez DAC, Gutierrez E, Dionisio EJ, Machado MAAM, Oliveira TM,
et al. Could chlorhexidine be an adequate positive control for antimicrobial photodynamic
therapy in- in vitro studies? Photodiagnosis and Photodynamic Therapy. 2019; 25: 58–62.
doi: 10.1016/j.pdpdt.2018.11.004.
30. Méndez DAC, Rizzato VL, Lamarque GCC, Dionisio EJ, Buzalaf MAR, Rios D, et al. Could
a chelant improve the effect of curcuminmediated photodynamic antimicrobial chemotherapy against dental intact biofilms? Lasers in Medical Science. 2019; 34(6): 1185–1192. doi: 10.1007/s10103-018-02708-x.
31. Panhóca V, Florez F, de Faria Júnior NB, de Souza Rastelli AN, Tanomaru J, Kurachi C, et
al. Evaluation of Antimicrobial Photodynamic Therapy against Streptococcus mutans Biofilm in situ. The Journal of Contemporary Dental Practice. 2016; 17(3): 184–191.
doi: 10.5005/jp-journals-10024-1825.
32. Merigo E, Conti S, Ciociola T, Manfredi M, Vescovi P, Fornaini C. Antimicrobial
photodynamic therapy protocols on streptococcus mutans with different
combinations of wavelengths and photosensitizing dyes. Bioengineering. 2019; 6(2): 42. doi: 10.3390/bioengineering6020042.
33. Chiniforush N, Pourhajibagher M, Shahabi S, Bahador A. Clinical approach of high
technology techniques for control and elimination of endodontic microbiota. Journal
of lasers in medical sciences. 2015; 6(4): 139–150. doi: 10.15171/jlms.2015.09.
34. Poppolo Deus F, Ouanounou A. Chlorhexidine in Dentistry: Pharmacology, Uses, and
Adverse Effects. International Dental Journal.2022; 72(3): 269–277.
doi: 10.1016/j.identj.2022.01.005.
35. Pereira JA, Polaquini CR, dos Santos V, Caiaffa KS, Rabelo RL, Theodoro R dos S, et al. Antibiofilm and cytotoxic effect of 3,3′-dihydroxycurcumin (DHC) as photosensitizer agent in antimicrobial photodynamic therapy for endodontic purposes. Photodiagnosis and Photodynamic
Therapy. 2021; 36: 102534. doi: 10.1016/j.pdpdt.2021.102534.
36. Araújo PV, Correia-Silva J de F, Gomez RS, Massara M de L de A, Cortes ME, Poletto LT de A. Antimicrobial effect of photodynamic therapy in carious lesions in vivo, using culture and real-time PCR methods. Photodiagnosis and Photodynamic Therapy. 2015; 12(3):
401–407. doi: 10.1016/j.pdpdt.2015.06.003.
37. Reis ACM, Regis WFM, Rodrigues LKA. Scientific evidence in antimicrobial photodynamic therapy: An alternative approach for reducing cariogenic bacteria. Photodiagnosis and Photodynamic Therapy. 2019; 26: 179–189.
doi: 10.1016/j.pdpdt.2019.03.012.
DOI: https://doi.org/10.22146/majkedgiind.94888
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