Evaluation of a Hand Antiseptic with WHO-Recommended Formulation and Its Efficacy in Killing Methicillin-Resistant Staphylococcus Aureus (MRSA)

  • Ahmad Subhan Clinical pharmacist Fatmawati General Hospital, Jakarta, Indonesia
  • Wasmen Manalu Departemen Anatomi Fisiologi dan Farmakologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor.
  • Min Rahminiwati Departemen Anatomi Fisiologi dan Farmakologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor.
  • Huda Salahudin Darusman Departemen Anatomi Fisiologi dan Farmakologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor.
Keywords: hand rub, hand sanitizer, ahmad Subhan, percentage kill, WHO

Abstract

The present pandemic that is caused by COVID-19 and previously by the clone of the methicillin-resistant staphylococcus aureus (MRSA) has threatened human life. This condition requires materials that can break the chain of transmission from human to human and from the environment to human. This study aimed to evaluate the quality of alcohol-based hand antiseptic using WHO-recommended formulation based on the stability of the formulation, the risk of irritation, and the ability to kill bacteria. Assessments on the presence of rancidity, clarity, discoloration, final alcohol content, and skin irritation risk were done to know the quality of the product. Methicillin-resistant staphylococcus aureus (MRSA) was used to assess the percentage of bacterial killing power. The selected bacteria were bacteria that are commonly found in the hospital environment. The results showed that from four variants tested, MK.IV had good stability compared to other formulations. In terms of irritation risk, twenty-three selected subjects could well tolerate the formula. The results of the killing efficacy against MRSA showed that the antiseptic could kill 99.90% of the bacteria at the 1st, 2nd, and 5th minute. A selected manufacturer's product also showed the same killing efficacy at the 1st, 2nd, and 5th minute. The effective value of the antiseptic for each contact time was ≥ 90%.

References

ASTM., (2008). Antimicrobial susceptibility testing method. Time – kill Test Protocol 786-071808E. 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. ASTM E 2315 – 03, p 3.
BPOM RI., (2008), Farmakope Indonesia (FI) edisi V., Badan Pengawas Obat dan Makanan (BPOM) Republik Indonesia, Jakarta.
Christoph, Ralf; Schmidt, Bernd; Steinberner, Udo; Dilla, Wolfgang; Karinen, Reetta., (2006). "Glycerol". Ullmann's Encyclopedia of Industrial Chemistry. Ullmann's Encyclopedia of Industrial Chemistry. doi:10.1002/14356007.a12_477.pub2. ISBN 3527306730.
Fanning S., (2011), basic medical key – disinfectants and antiseptics modes of action mechanisms of resistance and testing regimens; www.basicmedicalkey.com
FDA., (2018). Assessing the Irritation and Sensitization Potential of Transdermal and Topical Delivery Systems for ANDAs Guidance for Industry, 10001 New Hampshire Ave., Hillandale Bldg., 4th Floor Silver Spring, MD 20993-0002 301-796-3400; Fax: 301-431-6353 http://www.fda.gov/Drugs/GuidanceComplianceRegulatoryInformation/Guidances/default.htm
Fortuin-de Smidt MC, Singh-Moodley A, Badat R, Quan V, Kularatne R, Nana T, Lekalakala R, Govender NP, Perovic O, for GERMS-SA. (2015). Staphylococcus aureus bacteraemia in Gauteng academic hospitals, South Africa. Int J Infect Dis 30:41– 48. https://doi.org/10.1016/j.ijid.2014.10.0 11.
Gould D, Chamberlaine A. (1995). Staphylococcus aureus: a review of the literature. J Clin Nurs 4:5–12. https://doi.org/10.1111/j.13652702.1995.tb00004.x.
Haynes, William M., ed., 2011. CRC Handbook of Chemistry and Physics (92nd ed.). Boca Raton, FL: CRC Press. p. 3.246. ISBN 1439855110.
Instalasi Farmasi, (2018)., Laporan Tahunan Pengelolaan Perbekalan Farmasi RSUP Fatmawati, Instalasi Farmasi RSF.,Jakarta.
Katarzyna Leja, Katarzyna Czaczyk, Kamila Myszka, (2011). The use of microorganisms in 1,3-Propanediol production, African Journal of Microbiology Research Vol. 5(26), pp. 4652-4658, 16 November, 2011 Available online at http://www.academicjournals.org/AJMR ISSN 1996-0808 ©2011 Academic Journals DOI: 10.5897/AJMR11.847
Katayama Y, Ito T, Hiramatsu K. (2000). A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus. Antimicrob Agents Chemother 44: 1549–1555. https://doi.org/10.1128/AAC.44.6.1549 -1555.2000.
Kemenkes RI. (2020), Corona Virus Update., https://covid19.kemkes.go.id/
Lakhundi, S., & Zhang, K. (2018). Methicillin-Resistant Staphylococcus aureus: Molecular Characterization, Evolution, and Epidemiology. Clinical Microbiology Reviews, 31(4), 1–103.
Larson, E. L., Morton H. E., (1991). Alcohols. In S. S. Block (ed.), Disinfection, sterilization, and preservation, 4th ed. Lea & Febiger, Philadelphia, Pa. p. 422-434
Oladosu, P., Isu, N.R., Ibrahim, K., Okolo, P., Oladepo, D.K., (2013). Time kill-kinetics antibacterial study of Acacia nilotica. African Journal of Microbiology Research, 7(46): 5248-5252.
Wertheim HF, Melles DC, Vos MC, van Leeuwen W, van Belkum A, Verbrugh HA, Nouwen JL. (2005). The role of nasal carriage in Staphylococcus aureus infections. Lancet Infect Dis 5:751–762. https://doi.org/10.1016/S14733099(05)70295-4.
WHO (2020)., Coronavirus disease 2019 (COVID-19) Situation Report – 163., https://www.who.int/emergencies/diseases/novel-coronavirus-2019/situation-reports
WHO. (2009)., WHO guidelines in hand hygiene in health care. WHO/IER/PSP/2009.07, World Health Organisation, Geneva, Switzerland.
WHO., (2010). WHO guide to local production; WHO-recommended hand rub formulations. WHO revise April 2010.
Williams RE, Jevons MP, Shooter RA, Hunter CJ, Girling JA, Griffiths JD, Taylor GW. (1959). Nasal staphylococci and sepsis in hospital patients. Br Med J 2:658–662. https://doi.org/10.1136/bmj.2.5153.658.
Published
2021-01-27
How to Cite
Subhan, A., Manalu, W., Rahminiwati, M., & Darusman, H. S. (2021). Evaluation of a Hand Antiseptic with WHO-Recommended Formulation and Its Efficacy in Killing Methicillin-Resistant Staphylococcus Aureus (MRSA). Indonesian Journal of Pharmacy, 32(1), 114-125. https://doi.org/10.22146/ijp.1252
Section
Research Article