In Vivo Antihyperglycemic Study of Insulin Self-Nanoemulsifying Drug Delivery System (SNEDDS)
Keywords:
Keywords: Insulin, SNEDDS, Oral, Antihiperglycemic
Abstract
To deliver insulin orally requires an appropriate formulation strategy. Self nano-emulsifying drug delivery system (SNEDDS) can be used as an alternative to increase insulin bioavailability and activity. This study aim was to evaluate the antihyperglycemic activity of SNEDDS insulin. Insulin was loaded in SNEDDS by first dissolving it in glycerin. The dissolved content in glycerine was determined spectroscopically using the Bradford method. Insulin SNEDDS is then administered orally in rats in a variety of doses such as 43.39; 108,47; 216.94 IU / Kg. The rats used in this study were diabetic rats that had been induced with streptozotocin 48 mg / Kg BW. The insulin SNEDDS given can reduce blood glucose levels, while insulin without SNEDDS, PBS pH 7.4, and blank SNEDDS cannot lower blood glucose levels. The results of this study indicate that insulin given using the SNEDDS formulation can increase insulin activity, which is entirely ineffective if given orally without SNEDDS formulation.
Keywords: Insulin, SNEDDS, Oral, Antihiperglycemic
References
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Zhang, Q., Na, H., Zhang, L., Zhu, P., Chen, Q., Qiu, Y., dkk., 2012. The In vitro and In Vivo Study on SNEDDS Based on Insulin-Phospholipid Complex. Journal of Biomedical Nanotechnology, 8:90-7.
Belmin, J. dan Valensi, P., 2003. Novel drug delivery systems for insulin clinical potential for use in the elderly. Drugs Aging, 20:303-12.
Chalasani, K.B., Russell-Jones, G.J., Yandrapu, S.K., Diwan, P.V., dan Jain, S.K., 2007. A novel vitamin B12-nanosphere conjugate carrier system for peroral delivery of insulin. Journal of Controlled Release, 117: 421–429.
Deeds, M.C., Anderson, J.M., Amstrong, A.S., Gastineau, D.A., Hidding, H.J., Jahangir, A., dkk., 2011. Single dose streptozotocin-induced diabetes:considerations for study design in islet transplantation models. Laboratory Animals, 45:131-40.
Gursoy, R., N. dan Benita, S., 2004. Self-emulsifying drug delivery systems (SEDDS) for improved oral delivery of lipophilic drugs. Biomedicine & Pharmacotherapy, 58: 173–182.
Guyton, A.C. dan Hall, J.E., 2006. Textbook of Medical Physiology. Elsevier Saunders, Philadelphia.
Hamman, J., Enslin, G., dan Kotze, A., 2005. Oral delivery of peptide drugs barriers and developments. BioDrugs, 19(3):165-77.
Kadowaki, H., Nishitoh, H., Urano, F., Sadamitsu, C., Matsuzawa, A., Takeda, K., dkk., 2005. Amyloid beta induces neuronal cell death through ROS-mediated ASK1 activation. Cell Death and Differentiation, 12:19-24.
Khafagy, E.-S., Morishita, M., Onuki, Y., dan Takayama, K., 2007. Current challenges in non-invasive insulin delivery systems: A comparative review. Advanced Drug Delivery Reviews, 59: 1521–1546.
Kiernan, J.A., 1990. Histological & Histochemical Methods: Theory and Practice. Pergamon Press.
Leonard, T.W., Lynch, J., McKenna, M.J., dan Brayden, D.J., 2006. Promoting absorption of drugs in humans using medium chain fatty acid based solid dosage forms:GIPET. Expert Opinion on Drug Delivery, 3:685-92.
Li, P., Tan, A., Prestidge, C.A., Nielsen, H.M., dan Müllertz, A., 2014. Self-nanoemulsifying drug delivery systems for oral insulin delivery: In vitro and in vivo evaluations of enteric coating and drug loading. International Journal of Pharmaceutics, 477: 390–398.
Onuki, Y., Morishita, M., Takayama, K., Tokiwa, S., Chiba, Y., Isowa, K., dkk., 2000. In vivo effects of highly purified docosahexaenoic acid on rectal insulin absorption. International Journal of Pharmacy, 198(2):147-56.
Pringels, E., Callens, C., Vervaet, C., Dumont, F., Slegers, G., Foreman, P., dkk., 2006. Influence of deposition and spray pattern of nasal powders on insulin bioavailability. International Journal of Pharmaceutics, 310: 1–7.
Rees, D.A., dan Alcolado, J.C., 2005. Animal models of diabetes mellitus. Diabetic Medicine, 22:359-370.
Sadrzadeh, N., Glembourtt, M., dan Stevenson, C., 2007. Peptide drug delivery strategies for the treatment of diabetes. Journal of Pharmaceutical Science, 96:1925-54.
Selvam, R.P., Kulkarni, P.K., dan Dixit, M., 2013. Preparation and evaluation of self-nanoemulsifying formulation of efavirenz. Indian Journal of Pharmaceutical Education & Research, 47:47-54.
Sintov, A., Krymberk, I., Daniel, D., Hannan, T., Sohn, Z., dan Levin, G., 2007. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. Journal of Controlled Release, 89:311-20.
Todo, H., Okamoto, H., Lida, K., dan Danjo, K., 2001. Effect of additives on insulin absorption from intratracheally administered dry powders in rats. International Journal of Pharmaceutics, Vol 220:101-110.
Toschi, E., Camastra, S., Sironi, A.M., Masoni, A., Gastaldelli, A., Mari, A., dkk., 2002. Effect of acute hyperglycemia on insulin secretion in humans. Diabetes, Suppl 1:S130-3.
WHO, 2006. Definition and diagnosis of diabetes mellitus and intermediate hyperglycemia.
Winarti, L., Suwaldi, Martien, R., Hakim, L., 2018. Formulation Of Insulin Self Nanoemulsifying Drug Delivery System And Its In Vitro-In Vivo Study. Indonesian Journal Of Pharmacy 29(3):157-166
Zhang, Q., Na, H., Zhang, L., Zhu, P., Chen, Q., Qiu, Y., dkk., 2012. The In vitro and In Vivo Study on SNEDDS Based on Insulin-Phospholipid Complex. Journal of Biomedical Nanotechnology, 8:90-7.
Published
2026-05-04
How to Cite
Winarti, L., Martodiharjo, S., Martien, R., & Hakim, L. (2026). In Vivo Antihyperglycemic Study of Insulin Self-Nanoemulsifying Drug Delivery System (SNEDDS). ndonesian ournal of harmacy, 37(2), 381–389. https://doi.org/10.22146/ijp.2259
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Research Article
