Anti-Aging Face Cream Preparations Made from Collagen Membrane Chicken Eggshell (Gallus gallus domesticus) Combined with Bee Honey (Apis mellifera)
Abstract
Handling waste such as chicken egg shells requires innovation to be able to provide added value as a sustainable product. This study aims to extract collagen from chicken egg shell membranes combined with bee honey as an anti-aging face cream. This material has a very high model accuracy value (97.89%) which has the potential to inhibit aging due to Matrix metalloproteinase-1 (MMP-1). This research was carried out by extracting eggshell membrane collagen, making cream combined with bee honey, analyzing product properties, and interpreting virtual data. The results show that in 100 g of face cream formulation with active ingredients of chicken eggshell membrane collagen extract 4.1206% and bee honey 5.0251%; 77.0926% water; 0.0062% ash; 1,8690% protein; 0.0058% fat, the viscosity value was 65.94 dPas, and the pH was 7.08. Thus, this product did not irritate the skin of the experimental animals. The average value of attribute assessment (such as aroma, consistency, texture, stickiness, homogeneous sensation, color, appearance, itching, erythema, washing power, response after washing) was well received by the panelists with a score of 1-3,9 and the highest total achievement bacteria 25 CFU/g and yeast-mold 16 CFU/g met the skin moisturizer quality requirement (SNI 16-4399-1996) which should not exceed 102 CFU/g.
References
Alhana. 2015. Extraction and characterization of collagen and nanocollagen from the flesh of the gamma sea cucumber (Stichopus variegatus) [Bachelor thesis]. [Bogor]: Institut Pertanian Bogor.
Alhana, Suptijah P, Tarman K. 2015. Extraction and characterization of collagen from sea cucumber flesh. J. Pengolah. Has. Perikan. Indones. 18(2):150-161. doi:10.17844/jphpi.v18i2.10610.
Ali SM, Yosipovitch G. 2013. Skin pH: From basic science to basic skin care. Acta Derm. Venereol. 93: 261–267. doi:10.2340/00015555-1531.
American Academy of Dermatology. 2017. Slow The Signs of Aging with Sun Protection. URL https://www.aad.org/news/aging-and-sun-protection.
Badan Pusat Statistik. 2021. Laying Chicken Egg Production by Province (Tons), 2018-2020. URL https://www.bps.go.id/indicator/24/491/1/produksi-telur-ayam-petelur-menurut-provinsi.html.
Bayraktar O, Galanakis CM, Aldawoud TMS, Ibrahim SA, Kose DM, Uslu MU. 2021. Utilization of eggshell membrane and olive leaf extract for the preparation of functional materials. Foods. 10(4):806. doi:10.3390/foods10040806.
Faradiba, Faisal A, Ruhama M. 2013. Face cream formulation from lemon juice (Vitis vinifera L.) with various concentrations of emulsifier. MFDF Journal. 17(1): 17-20.
Kalman DS, Helwings S. 2020. The effect of oral hydrolyzed eggshell membrane on the appearance of hair, skin, and nails in healthy middle-aged adults: A randomized double-blind placebo-controlled clinical trial. J. Cosmet. Dermatol. 19(6):1463-1472. doi:10.1111/jocd.13275.
Pengawas Obat dan Makanan Republik Indonesia. 2020. In Vivo Preclinical Toxicity Test Guidelines. URL https://jdih.pom.go.id/download/flip/1192/-/2020.
Ponkham W, Limroongreungrat K, Sangnark A. 2011. Extraction of collagen from hen eggshell membrane by using organic acids. Thai J. Agric. Sci. 44(5):354-360.
Puspitasari AD, Mulangsri DAK, Herlina. 2018. Sunscreen cream formulation ethanol extract of cherry leaves (Muntingia calabura L.) for skin health. MPK Journal. 28(4): 263-270. doi: 10.22435/mpk.v28i4.524.
Safitri FW, Syahreza A, Farah HS, Satrio BMC, Hadi IS. 2016. Antioxidant activities and antioxidant cream formulation of corn silk (Zea Mays L) extract. SainsMed. 7(2):64-69. doi: 10.26532/sainsmed.v7i2.1176.
Sherwood L. 2007. Human physiology: From cells to systems. 6 th ed. Pacific Grove: Thomson/Brooks/Cole.
Sumarlin LO, Muawanah A, Wardhani P, Masitoh, 2014. Anticancer and antioxidant activity of honey in the market local Indonesia. Indones. J. Agric. Sci. 19(3): 136-144.
Turck D, Bresson JL, Burlingame B, Dean T, Fairweather-Tait S, Heinonen M, Hirsch-Ernst KI, Mangelsdorf I, McArdle HJ, Naska A, Neuhäuser-Berthold M, Nowicka G, Pentieva K, Sanz Y, Siani A, Sjodin A, Stern M, Tome D, Vinceti M, Willatts P, Engel KH, Marchelli R, Poting A, Schlatter JR, Ackerl R, van Loveren H. 2018. Safety of egg membrane hydrolysate as a novel food pursuant to Regulation (EU) 2015/2283. EFSA. 16(7):1-13. doi: 10.2903/j.efsa.2018.5363.
Xia W, Hammerberg C, Li Y, He T, Quan T, Voorhees JJ, Fisher GJ. 2013. Expression of catalytically active matrix metalloproteinase-1 in dermal fibroblasts induces collagen fragmentation and functional alterations that resemble aged human skin. Aging Cell. 12(4):661-71. doi:10.1111/acel.12089.
Yu Z, Zhan J, Wang H, Zheng H, Xie J, Wang, X. 2020. Analysis of influencing factors on viscosity of agar solution for capsules. J. Phys.: Conf. Ser. 1653(2020): 12059-12065. doi:10.1088/1742-6596/1653/1/012059.
Yumas M. 2016. The formulation of facial cream preparations with the active ingredient extract methanol of non-fermented cocoa beans (Theobroma cacao L.) a combination of bee honey. J. plantation based ind. 11(2):75-87. doi:10.33104/jihp.v11i2.3414.