Steroidal Saponin Isolated from Dioscorea alata L. Extract Improve Digestive Tract Allergy in Balb/C Mice Induced by Ovalbumin

  • Sri Nabawiyati Nurul Makiyah Universitas Muhammadiyah Yogyakarta
  • Sri Tasminatun School of Pharmacy, Faculty of Medicine and Health Sciences, Universitas Muhammadiyah Yogyakarta, Jl. Brawijaya, Geblagan, Tamantirto, Daerah Istimewa Yogyakarta 55183
  • Muhammad Sasmito Djati Biology Department, Faculty of Mathematics and Natural Science, Brawijaya University, Malang, Indonesia, Jawa Timur 65145
  • Muhaimin Rifa’i Biology Department, Faculty of Mathematics and Natural Science, Brawijaya University, Malang, Indonesia, Jawa Timur 65145
  • Widodo Widodo Biology Department, Faculty of Mathematics and Natural Science, Brawijaya University, Malang, Indonesia, Jawa Timur 65145
Keywords: allergy, inflammation, diosgenin, purple yam, steroidal saponin


Steroidal saponin compound in Dioscorea species is one of the crucial substance because it has some biological functions, such as immunomodulatory and anti-allergic agent. This study aims to analyze the effect of steroidal saponin of Dioscorea alata L. on CD4 T cells activation, cytokines IL-4, IL-5 and TGF-β. This study used primary lymphocyte cell which isolated from BALB/c mice spleen. Mice lymphocyte were cultured in the complete medium with presence of various concentrations (50, 25 and 12.5 µg/mL) of three isolate of steroidal saponin of D. alata for 24 h. Diosgenin was used as positive control. After 24 h of treatment, the activation of lymphocyte, IL-4 and IL-5 production was determined by Flowcytometry analysis. Data were analyzed using student t-test with p < 0.05 and p < 0.01. The results showed that the low concentration of Isolate 1 (12.5 µg/mL) was found to be effective to stimulate the activation of CD4 T cells, reduce the production of IL4 and IL-5 by CD4 T cells and increase TGF-β production. These results showed that a low concentration of steroidal saponin of D. alata could be considered as immunomodulatory and anti-allergic agent. Purification and identification of each isolate isolated from DAE is in progress.


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How to Cite
Makiyah, S. N. N., Tasminatun, S., Djati, M. S., Rifa’i, M., & Widodo, W. (2023). Steroidal Saponin Isolated from Dioscorea alata L. Extract Improve Digestive Tract Allergy in Balb/C Mice Induced by Ovalbumin. Indonesian Journal of Pharmacy, 34(1), 153-161.
Research Article