Lewati ke menu navigasi utama Lewati ke konten utama Lewati ke footer situs
Logo Header Halaman

Artikel penelitian

Vol 8 No 1 (2014): Volume 8, Number 1, 2014

Enzymatic hydrolysis of sorghum bagasse to readily fermentable sugar for bioethanol

DOI
https://doi.org/10.22146/rekpros.5019
Telah diserahkan
November 15, 2023
Diterbitkan
Juni 30, 2014

Abstrak

Produksi gula dari bagase sorghum menggunakan enzim selulase dan selobiase dilakukan dalam kultur batch. Tujuan percobaan adalah mempelajari pengaruh beban bagase sorghum dan waktu pretreatment kapur terhadap produksi gula dan yield gula. Pretreatment kapur dilakukan dalam 1000 ml labu leher tiga dengan beban kapur 0,1 g Ca(OH)2 /g sorghum bagasse dan ditambah dengan 500 ml air distilasi. Pengaruh waktu pretreatment (1, 2, 3, dan 4 jam) pada suhu 100°C dan pengaruh beban biomassa (5, 10, 15 % w/v) pada hidrolisis enzim untuk menghasilkan gula. Hasil penelitian menunjukkan bahwa konsentrasi gula maksimum dicapai sebesar 28,04 g/l dalam waktu pretreatment 4 jam; dan yield maksimum gula diperoleh 0,4 g glukose/ g biomassa dengan beban biomassa 5% (w/v).

Referensi

  1. Duff, S. J. B., dan Murray, W. D., 1996. Bioconversion of forest products industry waste cellulose to fuel ethanol: A Review. Bioresour. Technol., 55, 1-33.
  2. Digman, M. F., Shinners, K. J., Dien, B. S., Muck, R. E., Xin-Liang, L., Hatfield, R. D.,Weimer, P. J., 2007. On-farm Pretreatment Technologies for Improving Enzymatic Degradability of Cellulose and Hemicellulose Present in Perennial Grass, ASABE Annual International Meeting, Minneapolis, MN.
  3. Galbe, M., Zacchi, G., 2007. Pretreatment of lignocellulosic materials for efficient bioethanol production. Adv. Biochem. Eng.: Biotechnol. 108, 41–65.
  4. Hendriks, A. T. W. M., Zeeman, G., 2009. Pretreatments to enhance the digestibility of lignocellulosic biomass. Bioresour. Technol. 100, 10–18.
  5. International Starch Institute (ISI), 1999. Determination of Reducing Power and DE by Lane and Eynon’s method. ISI 27-1e.
  6. Lin, Y., Tanaka, S., 2006. Ethanol fermentation from biomass researches: current state and prospects, Appl. Microbiol, Biotechnol. 69, 627–642.
  7. Li, C., Wang, Q., Zhao, Z. K., 2008. Acid in ionic liquid: an efficient system for hydrolysis of lignocellulose, Green Chem., 10, 177–182.
  8. Lynd, L. R., Laser, M. S., Brandsby, D., Dale, B. E., Davison, B., Hamilton, R., Himmel, M., Keller, M., McMillan, J. D., Sheehan, J., Wyman, C. E., 2008. How biotech can transform biofuels, Nat. Biotechnol. 26, 169–172.
  9. McIntosh, S. and Vancov, T., 2010. Enhanced enzyme saccharification of Sorghum bicolor straw using dilute alkali pretreatment. Biotechnol, 101, 6718– 6727.
  10. Mosier, N., Hendrickson, R., Ho, N., Sedlak, M., Ladisch, M.R., 2005. Optimization of pH controlled liquid hot water pretreatment of corn stover, Bioresour. Technol. 96, 1986–1993.
  11. Mosier, N., Wyman, C. E., Dale, B. E., Elander, R., Lee, Y. Y., Holtzapple, M., 2005b. Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresour. Technol. 96 (6), 673–686.
  12. Silverstein, R. A., Chen, Y., Sharma-Shivappa, R. R., Boyette, M. D., Osborne, J., 2007. Acomparison of chemical pretreatment methods for improving saccharification of cotton stalks, Bioresour Technol., 98, 3000–3011.
  13. Wyman, C. E., Dale, B. E., Elander, R. T., Holtzapple, M., Ladisch, M. R., Lee, Y. Y., 2005. Coordinated development of leading biomass pretreatment technologies, Bioresour. Technol. 96, 1959–1966.
  14. Xu, J. dan Cheng, J. J. 2011. Pretreatment of switchgrass for sugar production with the combination of sodium hydroxide and lime. Bioresour. Technol., 102, 3861–3868.