Fucoxanthin-Rich Padina australis: Anti-Ageing Properties Through Enzyme Inhibition
Abstract
The brown seaweed, Padina australis Hauck (PAH), known for its high carotenoid content, has been shown to mitigate skin ageing by inhibiting collagenase activity. This study aims to evaluate the inhibitory effects of PAH on collagenase and tyrosinase enzymes. The study begins by extracting the seaweed using ethanol. Afterwards, it is fractionated with ethyl acetate to obtain ethyl acetate-soluble and ethyl acetate-insoluble compounds (EAS and EAI, respectively). The crude extract and both fractions were then subjected to assays to determine their collagenase and tyrosinase inhibitory activities. The results demonstrated that the isolation exhibited the highest inhibition of both enzymes, followed by the EAS, with the EAI showing the least activity. Thin-layer chromatography (TLC) analysis of both fractions revealed retardation factors (Rf identical) to the standard fucoxanthin. TLC densitometry analysis quantified fucoxanthin concentrations at 42.36 ± 3.94 µg mg-1 in the extract and 99.104 ± 7.76 µg mg-1 in the EAS. Furthermore, the study concluded that the extract, EAS, and isolate had IC50 values below 100 µg mL-1, categorising them as effective inhibitors. The high fucoxanthin content in the extract and fractions suggests significant potential for anti-ageing applications.
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