Leaf Anatomical Structure of Five True Mangrove Species in Pemogan Village, Denpasar – Bali

I Made Saka Wijaya; Ni Kadek Rika Pramesti; Ni Made Gari

doi:10.22146/jtbb.16575

I Made Saka Wijaya Biology Program, Faculty of Mathematics and Natural Sciences, Udayana University. Jl. Raya Kampus Unud Jimbaran, Kuta Selatan, Badung – Bali 80361; Environmental Research Centre (PPLH) of Udayana University. Jl. PB Sudirman, Denpasar Barat, Denpasar – Bali 80234 https://orcid.org/0000-0003-2427-3401
Ni Kadek Rika Pramesti Biology Program, Faculty of Mathematics and Natural Sciences, Udayana University. Jl. Raya Kampus Unud Jimbaran, Kuta Selatan, Badung – Bali 80361
Ni Made Gari Biology Program, Faculty of Mathematics and Natural Sciences, Udayana University. Jl. Raya Kampus Unud Jimbaran, Kuta Selatan, Badung – Bali 80361

DOI: https://doi.org/10.22146/jtbb.16575

Keywords: Amphistomatic, Hypostomatic, PCA, Stomata, Transversal Section

Abstract

Anatomy is an approach to study physiological processes that occurs in plants. Even though the mangrove community is known as one of the communities with the largest carbon stock, data regarding the anatomical structure of its leaves is still insufficient. Therefore, this research aims to determine the anatomical structure of leaves in several mangrove species and to compare those structures. Samples of mangrove species were obtained from Simbar Segara Batu Lumbang mangrove area in Pemogan Village, Denpasar City, Bali. The species are used i.e. (1) Lumnitzera racemosa (Combretaceae) in landward zone; (2) Xylocarpus granatum (Meliaceae) in landward zone; (3) Aegiceras corniculatum (Primulaceae) in middle zone; (4) Rhizophora apiculata (Rhizophoraceae) in middle zone; and (5) Sonneratia alba (Lythraceae) in seaward zone. Anatomical data was obtained by the free hand section method through the midrib and a paradermal method. The data was analysed descriptively, then continued with principal component analysis (PCA). Based on the results, L. racemosa and S. alba are species with amphistomatic (the other species were hypostomatic) and isobilateral leaves (the others were dorsiventral). These two characters are the dominant in PCA. Epidermal tissue, palisade mesophyll, sponge mesophyll, vascular bundles, and calcium oxalate crystals are evenly found in all species. Rhizophora apiculata, A. corniculatum, and X. granatum have hypodermal tissue as a dominant character in PCA. Secretory tissue and salt glands are only observed in A. corniculatum. The five species have three different stomata types, namely tetracytic (L. racemosa), anomocytic (X. granatum, A. corniculatum, R. apiculata), and staurocytic (S. alba).

References

Backer, C.A. & van den Brink, R.C.B., 1963. Flora of Java vol I, Netherlands: Springer Netherlands.

Chorchuhirun, B., Kraichak, E., & Kermanee, P., 2020. Comparative anatomy of two mangrove species: Xylocarpus granatum and Xylocarpus moluccensis (Meliaceae). Thai Journal of Science and Technology, 9(3), pp.355–367. doi: 10.14456/tjst.2020.25.

Evans, L.S. & Bromberg, A., 2010. Characterization of cork warts and aerenchyma in leaves of Rhizophora mangle and Rhizophora racemosa. Journal of the Torrey Botanical Society, 137(1), pp.30–38. doi: 10.3159/09-RA-024.1.

Filartiga, A.L. et al., 2022. Comparative anatomy of leaf petioles in temperate trees and shrubs: the role of plant size, environment and phylogeny. Annals of Botany, 129(5), pp.567–582. doi: 10.1093/aob/mcac014.

Franceschi, V.R. & Nakata, P.A., 2005. Calcium Oxalate in Plants Formation and Function. Annual Review of Plant Biology, 56, pp.41-71. doi: 10.1146/annurev.arplant.56.032604.144106

Hetherington, A.M. & Woodward, F.I., 2003. The role of stomata in sensing and driving environmental change. Nature, 424, pp.901-908. doi: 10.1038/nature01843.

Irawan, A., Chikmawati, T. & Sulistikorini, 2021. Diversity and zonation of mangrove flora in Belitung Island, Indonesia. Biodiversitas, 22(5), pp.2981-2992. doi: 10.13057/biodiv/d220563.

Karabourniotis, G. et al., 2021. The Optical Properties of Leaf Structural Elements and Their Contribution to Photosynthetic Performance and Photoprotection. Plants, 10, 1455. doi: 10.3390/plants10071455.

Kauffman, J.B. et al., 2020. Total ecosystem carbon stocks of mangroves across broad global environmental and physical gradients. Ecological Monographs, 90(2), 1405. doi: 10.1002/ecm.1405.

Khan, A. et al., 2022. Limited acclimation in leaf morphology and anatomy to experimental drought in temperate forest species. Biology, 11(8), 1186. doi: 10.3390/biology11081186.

Kitamura, S. et al., 1997. Handbook of Mangroves in Indonesia: Bali and Lombok, International Society for Mangrove Ecosystem, Japan.

Lux, A., Vaculík, M. & Kováč, J., 2015. Improved methods for clearing and staining of plant samples. In Plant Microtechniques and Protocols. Switzerland: Springer International Publishing, pp.167–178. doi: 10.1007/978-3-319-19944-3.

Mursyid, H. et al., 2022. Urgensi Pelestarian Ekosistem Lingkungan Fisik pada Destinasi Wisata: Pengalaman dari Kawasan Ekowisata Hutan Hangrove Batu Lumbang, Denpasar, Bali. Jumpa, 9(1), pp.451-477. doi: 10.24843/JUMPA.2022.v09.i01.p19.

Naskar, S., Mondal, S. & Ankure, S., 2021. Leaf anatomical adaptations of mangroves. In Handbooks of Halophytes. Switzerland: Springer International Publishing, pp.1064-1074. doi: 10.1007/978-3-030-57635-6.

Pankhurst, R.J., 1991. Practical taxonomic computing, New York: Cambridge University Press.

Parida, A.K., Das, A.B. & Mittra, B., 2004. Effects of salt on growth, ion accumulation, photosynthesis and leaf anatomy of the mangrove, Bruguiera parviflora. Trees, 18, pp.167-174. doi: 10.1007/s00468-003-0293-8.

Parida, A.K. & Jha, B., 2010. Salt tolerance mechanisms in mangrove: a review. Trees, 24, pp.199-217. doi: 10.1007/s00468-010-0417-x.

Poole, I. & Kurschner, W.M., 1999. Stomatal density and index: the practice. In Fossil Plants and Spores: modern techniques, Geological Society London, pp.257-260.

Raeski, P.A. et al., 2023. Applications of calcium oxalate crystal microscopy in the characterization of Baccharis articulata. Brazilian Archives of Biology and Technology, 66, 23230078. doi: 10.1590/1678-4324-ssbfar-2023230078.

Rao, T.A. & Bhupal, O.P., 1973. Typology of sclereids. Proceedings of the Indian Academy of Sciences, 77(2), pp.41–55. doi: 10.1007/BF03045552.

Royer, D.L., 2001. Stomatal density and stomatal index as indicators of paleoatmospheric CO2 concentration. Review of Palaeobotany and Palynology, 114(1-2), pp.1–28.

Rugayah et al., 2004. Pengumpulan Data Taksonomi. In Pedoman Pengumpulan Data Keanekaragaman Flora. Puslit Biologi – LIPI Bogor, pp.5–42.

Sabandar, A., Hiariej, A. & Sahertian, D.E., 2021. Struktur sel epidermis dan stomata Aegiceras corniculatum dan Rhizophora apiculata di muara sungai Desa Poka dan Desa Laehari. Biology Science & Education, 10(1), pp.81-87. doi: 10.33477/bs.v10i1.1896

Sánchez, A.R. et al., 2021. Influence of edaphic salinity on leaf morphoanatomical functional traits on juvenile and adult trees of red mangrove (Rhizophora mangle): Implications with relation to climate change. Forests, 12, 1586. doi: 10.3390/f12111586.

Sokal, R.R. & Sneath P.H.A., 1963. Principles of Numerical Taxonomy, San Francisco: WH Freeman and Company.

Strock, C.F., Schneider, H.M. & Lynch, J.P., 2022. Anatomics: High-throughput phenotyping of plant anatomy. Trends in Plant Science, 27(6), pp.520–523. doi: 10.1016/j.tplants.2022.02.009.

Tatongjai, S., Kraichak, E. & Kermanee, P., 2021. Comparative Anatomy and Salt Management of Sonneratia caseolaris (L.) Engl. (Lythraceae) Grown in Saltwater and Freshwater. PeerJ, 9, 10962. doi: 10.7717/peerj.10962.

Tihurua, E.F., Agustiani, E.L., & Rahmawati, K., 2020. Character of leaf anatomy as form of plant adaptation on mangrove zonation in Banggai Islands, Central Celebes. Jurnal Kelautan Tropis, 23(2), pp.255-264. doi: 10.14710/jkt.v23i2.7048.

Tihurua, E.F. et al., 2023. Leaf anatomical characters of several true mangrove species. Berita Biologi, 22(1), pp.11-128. doi: 10.14203/beritabiologi.v20i1.3991.

Tobing, A.N.L. et al., 2022. Anatomical adaptation of grey mangrove (Avicennia marina) leaf in the pond and coast located in Mangunharjo, Semarang, Central Java. Biosaintifika, 14(1), pp.57-64. doi: 10.15294/biosaintifika.v14i1.29828.

Tooulakou, G. et al., 2016. Alarm Photosynthesis: Calcium Oxalate Crystals as an Internal CO2 Source in Plants. Plant Physiology, 171(4), pp.2577-2585. doi: 10.1104/pp.16.00111.

Vinoth, R., Kumaravel, S. & Ranganathan, R., 2019. Anatomical and physiological adaptation of mangrove wetlands in east coast of Tamil Nadu. World of Scientific News, 129, pp.161–179.

Wang, W., Li, X. & Wang, M., 2019. Propagule dispersal determines mangrove zonation at intertidal and estuarine scales. Forest, 10, 245. doi:10.3390/f10030245.

Zhu, J. et al., 2018. Rapid estimation of stomatal density and stomatal area of plant leaves based on object-oriented classification and its ecological trade-off strategy analysis. Forests, 9(10), 616. doi: 10.3390/f9100616.