Upregulation of Glucomannan Synthesis Genes in Porang (Amorphophallus muelleri Blume) after Polyploidy Induction using Colchicine

Didik Wahyudi; Suyono; Ruri Siti  Resmisari; Nida Annisa  Sholeha; Sabrina Labista  Wibowo; Devi Rahmawati  Kafara

doi:10.22146/jtbb.19849

Didik Wahyudi Plant Physiology Laboratory, Biology Department, Faculty of Science and Technology Universitas Islam Negeri Maulana Malik Ibrahim Malang, Jl. Gajayana No.50, Malang City, East Java 65144, Indonesia https://orcid.org/0000-0001-6020-9365
Suyono Plant Physiology Laboratory, Biology Department, Faculty of Science and Technology Universitas Islam Negeri Maulana Malik Ibrahim Malang, Jl. Gajayana No.50, Malang City, East Java 65144, Indonesia https://orcid.org/0009-0008-7852-2956
Ruri Siti Resmisari Plant Tissue Culture Laboratory, Biology Department, Faculty of Science and Technology Universitas Islam Negeri Maulana Malik Ibrahim Malang, Jl. Gajayana No.50, Malang City, East Java 65144, Indonesia https://orcid.org/0000-0001-6623-5257
Nida Annisa Sholeha Plant Tissue Culture Laboratory, Biology Department, Faculty of Science and Technology Universitas Islam Negeri Maulana Malik Ibrahim Malang, Jl. Gajayana No.50, Malang City, East Java 65144, Indonesia
Sabrina Labista Wibowo Plant Tissue Culture Laboratory, Biology Department, Faculty of Science and Technology Universitas Islam Negeri Maulana Malik Ibrahim Malang, Jl. Gajayana No.50, Malang City, East Java 65144, Indonesia
Devi Rahmawati Kafara Plant Tissue Culture Laboratory, Biology Department, Faculty of Science and Technology Universitas Islam Negeri Maulana Malik Ibrahim Malang, Jl. Gajayana No.50, Malang City, East Java 65144, Indonesia

DOI: https://doi.org/10.22146/jtbb.19849

Keywords: Colchicine, Glucomannan biosynthesis, Polyploid porang, Porang cultivation

Abstract

Inducing polyploidy in porang using colchicine has been explored and successfully enhanced the genetic variability of porang. However, the specific impact of polyploidy on the expression of the genes responsible for glucomannan synthesis in porang has not been extensively studied. Therefore, this study aims to evaluate the effect of polyploidy induction on the expression of genes responsible for glucomannan synthesis in porang. Porang shoot explants (2n = 26) were used for polyploidy induction. Polyploidy induction was carried out using colchicine added to the culture medium. The concentrations of colchicine used in this study were 0 %, 0.01 %, 0.02 %, 0.03 %, and 0.04 %. After 53 days in media without colchicine, explants were collected for flow cytometry analysis and RNA isolation. The application of colchicine at various concentrations induced tetraploid and mixoploid porang. Mutation induction using colchicine also increases the expression (upregulation) of the CSLA, GMPP, CSLD, and GMPP genes encoding glucomannan biosynthesis in porang. Tetraploid (0.01 % colchicine treatment) and mixoploid porang (0.02 % colchicine treatment) showed the highest expression of genes involved in glucomannan biosynthesis via both sucrose and invertase pathways.

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