Antibacterial Activity of Myrmecodia pendens (Ant Nest Plant) against Multidrug Resistance Bacteria from Clinical Samples

https://doi.org/10.22146/mot.110761

Muhammad Evy Prastiyanto(1*), Aditya Rahman Ernanto(2), Sri Darmawati(3), Endang Tri Wahyuni Maharani(4), Arif Mulyanto(5), Rizal Maarif Rukmana(6), Wahyu Aristyaning Putri(7), Lia Kusmita(8), Shiv Kumar Sah(9), Daniel Geleta(10)

(1) Department of Medical Laboratory Technology, Faculty of Health and Nursing Science, Universitas Muhammadiyah Semarang, Semarang
(2) Department of Medical Laboratory Technology, Faculty of Health and Nursing Science, Universitas Muhammadiyah Semarang, Semarang
(3) Department of Medical Laboratory Technology, Faculty of Health and Nursing Science, Universitas Muhammadiyah Semarang, Semarang
(4) Department of Chemistry Education, Universitas Muhammadiyah Semarang, Semarang
(5) Department of Medical Laboratory Technology, Faculty of Health Sciences, Universitas Muhammadiyah Purwokerto
(6) Research Center for Pharmaceutical Ingredients and Traditional Medicine, Research Organization for Health, National Research and Innovation Agency (BRIN), Bogor, 16911
(7) Department of Tropical Biology, Universitas Gadjah Mada, Yogyakarta
(8) STIFAR Yayasan Pharmasi Semarang, Jl. Letjend Sarwo Edhie Wibobo Km 1, Semarang
(9) Department of Pharmacy, Maharajgunj Medical Campus (MMC), Institute of Medicine (IOM), Tribhuvan University, Kathmandu, Bagmati
(10) Department of Medical Laboratory Sciences, Jimma University, Jimma, Oromia
(*) Corresponding Author

Abstract


The emergence of multidrug-resistant (MDR) bacteria causes a substantial barrier to treatment, demanding the development of new antibacterial medicines. Myrmecodia pendens (ant nest plant), an indigenous medicinal species, is esteemed for its potential medicinal properties. This study aimed to examine the antibacterial efficacy of M. pendens extracts against MDR bacterial strains (Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Citrobacter freundii, and Pseudomonas aeruginosa) obtained from clinical samples. Extracts were prepared via maceration using ethanol, chloroform, and n-hexane solvents. Antibacterial efficacy was evaluated using the agar diffusion method, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) assays. The ethanol solvent extract exhibited the most significant antibacterial activity, particularly against P. aeruginosa, with an inhibition zone of 23.15 ± 0.21 mm at 100 mg/mL. The MIC and MBC values were 3.13 mg/mL and 12.5 mg/mL, respectively. The chloroform and n-hexane extracts showed lower, but comparable, inhibitory effects against the tested strains. These findings provide compelling evidence that M. pendens extracts, especially the ethanol extracts, represent a promising source for developing alternative antibacterial agents to combat MDR infections.


Keywords


Antibacterial; Ant Nest; Myrmecodia pendens; Pseudomonas aeruginosa

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DOI: https://doi.org/10.22146/mot.110761

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