THE EFFECTS OF Jatropha curcas L SEED EXTRACT IN REGULATION EXPRESSION TUMOR MARKER OF TGF- β1 GENE

https://doi.org/10.22146/tradmedj.24304

Endah Wulandari(1*), Rr. Ayu Fitri Hapsari(2)

(1) Department of Biochemistry, Faculty of Medicine and Health of Islamic State University Syarif Hidyatullah, Jakarta
(2) Department of Histology, Faculty of Medicine and Health of Islamic State University Syarif Hidyatullah, Jakarta
(*) Corresponding Author

Abstract


The role of TGF-β1 is known as the main immunosuppresor associated with tumor, but on the other opinion, it is associated with proliferation and tumor invasion. The increase and decrease of the secretion of TGF-β is to regulate the proliferation, differentiation, and death of various cell types. Now we all know the extract of Jatropha curcas L seed serves as antitumor. Allegedly, it can regulate the expression of TGF-β1 in control of cell number. The purpose of this study is to determine the effects of Jatropha seeds to the regulation of gene expression of TGF-β1 as a tumor marker. The method is performed by giving a dose groups the extract of jatropha seed (0, 5, 25, 50, 250 mg/BB) in mice. Then measurement of mRNA expression (RT-PCR), the protein of TGF-β1 levels (ELISA), and qualitative observations of liver histology were done. The expression of TGF-β1 mRNA is significantly 4.39 to 7.34 times higher than (ANOVA, p 0.05) than the control. Histological observation of liver showed the extract of jatropha seed induces damage nucleus of hepatocytes cell and sinusoidal. The effects extract of jatropha seed increased the level of TGF-β1 mRNA but not followed by increasing protein of TGF-β1 levels, and it was stimulated necrosis and apoptosis of hepatocytes cell.

Keywords


TGF-β1; Jatropha curcas L; tumor



References

Agbogidi, O.M., Akparobi, S.O., and Eruotor, P.G. 2013. Health and environmental benefits of Jatropha curcas linn. App Sci Rep. 1(2):36-39.

Choud J., Zavadova E., Halaska M.J., Strnad P., Fucikova T., and Rob L. 2008. Preoperative transforming growth factor-beta 1 (TGF-beta 1) plasma levels in operable breast cancer patients. Eur J Gynaecol Oncol. 29(6):613-6.

Guicciardi, M.E., Malhi, H., Mott, J.L., and Gores, G.J. 2013. Apoptosis and necrosis in the liver. Compr Physiol. DOI: 10.1002/sphy.c1200020.

Han, J., and Alvarez-Breckenridge, C.A., 2015. TGF-β1 signaling and its targeting for glioma treatment. Am J Cancer Res. 5(3);945-955.

Herman, D., Thomas, C.M., and Stekel, D.J. 2012. Adaptation for protein synthesis efficiency in a naturally acurring self-regulating operon. Pllos One. DOI:10.137/j.pone0049678.

Kisseleva, T., and David, A.B. 2008. Mechanisms Of Fibrogenesis. Exp Biol Med. 233:109–122

Lei, Z.X., Yin, J.D., Chang, W.J., Li, L.J., Zhong, L.Z., and Long, C.J. 2011. Transforming growth factor-1 phage model peptides isolated from a phage display 7-mer peptide library can inhibit the activity of keloid fibroblasts.Chinese Medical Journal. 124(3):429-435.

Liang, Yu., Zhu, F., Zhang, H., Chen, D., Zhang, X., Gao, Q., and Li, Y. 2016. Conditional of TGF-β signaling inhibits tumor progression and invasion in an induced mouse bladder cancer model. ScientificnReports.DOI:10.1038/srep29479.

Novitskiy, S.V., Forrester, E., Pickup, M.W., Gorska, A.E., Chytil, A., Aakre, M., Polosukhina, D., Owens, P., Yusupova, D.R., Zhao, Z., Shyr, Y., and Moses, H. 2014. Attenuated transforming growth factor beta signaling promotes metastasis in a model of HER2 mammary carcinogenesis. Breast cancer Res. 16:4-25.

Ringer, SA. 2013. Core concepts: thermoregulation in the newborn, part II: prevention of aberrant body temperature. NeoReviews. 13(14):221-229.

Yang, S., Yao, H., Li, M., Li, H., and Wang, F. 2016. Long non-coding RNA MALAT1 mediates Transforming Growth Factor Beta1-induced epithelial-Mesenchymal transition of retinal pigment epithelial cells. PloS One. DOI:10.1371/j.pone0152687.

Yin, Q., Liu, S., Dong, A., Mi, X., Hao, F., and Zhang, K. 2016. Targeting Transforming Growth Factor-Beta1 (TGF-β1) inhibit tumorigenesis of anaplastic thyroid carcinoma cell through ERK1/2-NF-ĸβ-PUMA signaling. Med Sci Monit. 22:2267-2277.

Zhang, Y., Nicholatos, J., Dreier, J.R., Ricoult, S.J.H., Widenmaler, S.B., Hotamisligil, G.S., Kwiatkowski, D.J., and Manning, B.D. 2014. Coordinated regulation of protein synthesis and degradation by mTORC1. Nature. 513:440-443.



DOI: https://doi.org/10.22146/tradmedj.24304

Article Metrics

Abstract views : 546 | views : 3035

Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

©Majalah Obat Tradisional (Trad.Med.J) 
Faculty of Pharmacy
Universitas Gadjah Mada