The Effect of Papaya Seed Ethanol Extract in Vivo on The Number of Osteoblasts Cells of Periodontitis-Induced Rats

Ratih Pusporini(1*), Ahmad Basori(2), Agung Krismariono(3)

(1) Department of Oral Biology, Faculty of Dentistry, Universitas Brawijaya
(2) Department of Pharmacology, Faculty of Medicine, Universitas Airlangga
(3) Department of Periodontics, Faculty of Dentistry, Universitas Airlangga
(*) Corresponding Author


The papaya seed ethanol extract is rich in antioxidant ingredients, such as flavonoids and phenolic acids. One of the main factors causing chronic inflammatory in periodontitis is oxidative stress. Administration of papaya seed extract is assumed to increase the number of rat osteoblast cells induced periodontitis. This research was conducted to analyze the effect of papaya seed extract on osteoblasts cells of rat induced periodontitis. This research represented an experimental laboratory-based investigation involving 35 rats of the Rattus norvegicus strain divided into 5 treatment groups (K, P1, P2, P3, P4). Control group (K) was not induced by periodontitis and was not given an extract, while group P induced periodontitis using LPS Phorphyromonas gingivalis (P.gingivalis) for 7 days and continued wire mesh installation around the mandibular incisors in the form of number “8” for 7 days. P1 group was given feed only without extract, while the rest were given extract of 200 mg/kgBB, 300mg/kgBB, and 400mg/kgBB. The data obtained were analyzed with a one-way ANOVA test. The results showed that the average number of osteoblasts varied significantly between the groups. There was an increase in the average number of osteoblast cells in rat induced periodontitis after given papaya seed extract.


periodontitis; papaya seed extract; osteoblast; flavonoid; phenolic acid

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Dhotre PS.,Suryakar AN., Bhogade RB., 2012, Oxidative Stress in Periodontitis, Eur J Gen Med;9(2):81-84.

Hajishengallis G. 2015, NIH Public Access.;35(1):3–11. plus J Dent Res 92(8):702-708, 2013.

Yoshida M, Akagawa Y., 2011,The relationship between tooth loss and cerebral stroke. Jpn Dent Sci Rev [Internet].; 47(2):157–60. Available from: 1016/j.jdsr.2011.01.001.

Najeeb S, Zafar MS, Khurshid Z, Zohaib S, Almas K.,2016, The role of nutrition in periodontal health: an update. Nutrients. Aug 30;8(9):530.

Kala BS, Gunjan C, Disha N, Shobha P.,2015, Treatment of periodontal disease-a herbal approach. Int. J. Pharm. Sci. Rev. Res.;33:126-36.

Tapsoba H, Deschamps JP.,2006, Use of medicinal plants for the treatment of oral diseases in Burkina Faso. J Ethnopharmacol.104(1–2):68–78.

Kothari V, Seshadri S.,2010, Antioxidant activity of seed extracts of Annona squamosa and Carica papaya. Nutr Food Sci [Internet]. 40(4):403–8. Available from: http://

Bansal N& Gupta N.,2014, Role Of Dietary Antioxidants In Periodontitis: A Preventive Approach. IOSR Journal of Dental and Medical Sciences. 13. 81-84. 10.9790/0853-13968184.

Rahman I, Biswas SK, Kirkham PA.,2006, Regulation of inflammation and redox signaling by dietary polyphenols. Biochem Pharmacol.;72(11):1439–52.

Amazu LU, Azikiwe CCA, Njoku CJ, Osuala FN, Nwosu PJC, Ajugwo AO, et al.,2010, Antiinflammatory activity of the methanolic extract of the seeds of Carica papaya in experimental animals. Asian Pac J Trop Med.;3(11):884–6.

Hienz SA, Paliwal S, Ivanovski S., 2015, Mechanisms of bone resorption in periodontitis. J Immunol Res.;http://

Chen D1, Nie M, Fan MW, Bian Z., 2008, Anti-inflammatory activity of curcumin in macrophages stimulated by lipopolysaccharides from Porphyromonasgingivalis. Pharmacology, 82(4):264-9. doi: 10.1159/000161127.

Ionel A, Lucaciu O, Moga M, Buhatel D, Ilea A, Catoi C, et al. 2015, Periodontal disease induced in Wistar rats - experimental study. Hum Vet Med Int J Bioflux Soc. 7(2):90–5.

Peter JK, Kumar Y, Pandey P, Masih H. 2014, Antibacterial Activity of Seed and Leaf Extract of Carica Papaya var. Pusa dwarf Linn. IOSR J Pharm Biol Sci [Internet]. 9(2):29–37. Available from: http://www. pdf.

Wulansari DD, Basori A, Suhartati S., 2017, Trad. Med. J., May - August Vol. 22(2), p 131-138.

Fuadiyah D, Ratnawaty R, Soebijakto BA.,2017, Pengaruh lama paparan lps (lipopolisakarida) phorpyromonas gingivalis sebagai induktor periodontitis terhadap kadar ldl dan hdl tikus wistar (rattus norvegicus), e-prodenta, vol 1, no 2 pp. 54-67.

Kato H, Taguchi Y, Tominaga K, Umeda M, Tanaka A., 2014, Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells. Arch Oral Biol [Internet].59(2):167–75. 10.1016/ j.archoralbio.2013.11.008.

Köse O, Arabaci T, Kizildag A, Erdemci B, Özkal Eminoğlu D, Gedikli S, et al. Melatonin prevents radiation-induced oxidative stress and periodontal tissue breakdown in irradiated rats with experimental periodontitis. J Periodontal Res. 2017;52(3):438–46.

Srinivasan PC. 2013; The Role of Inflammatory Cytokines and the RANKL-RANK-OPG Molecular Triad in Periodontal Bone Loss-A Review. J Clin Cell Immunol [Internet]. Available from: https://www.omicsonline .org/ the - role - of - inflammatory – cytokines – and – the – ranklrankopg -molecular – triad – in – periodontal- bone -loss-2155-9899.S13-007.php?aid=19772.

Azhar IS, Kresnoadi U, Rahayu RP. 2017;Potency of Garcinia mangostana L peel extract combined with demineralized freeze-dried bovine bone xenograft on IL-1 β expression, osteoblasts, and osteoclasts in alveolar bone. Dent J (Majalah Kedokt Gigi). 50(32):166–70.

Trzeciakiewicz A, Habauzit V, Horcajada MN, 2009;When nutrition interacts with osteoblast function: Molecular mechanisms of polyphenols. Nutr Res Rev. 22(1): 68–81.


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