Antibacterial Activity of Cuttlefish Sepia sp. (Cephalopoda,) Ink Extract Against Aeromonas hydrophila

R Adharyan Islamy(1*)

(1) Departement of Aquaculture, Faculty of Fisheries and Marine Science, Brawijaya University Malang
(*) Corresponding Author


Cephalopods ink has shown potential antiretroviral activity. The ink extracts of cuttlefish showed an antibacterial effect. This study aims to investigate the antibacterial activity of the methanolic extract of the ink of cuttlefish Sepia sp. against Aeromonas hydrophila. A. hydrophila are opportunists and associated with aquatic fish and shrimp disease. The shade-dried ink sample from approximately 30g ink sacs obtained from 15 animals was immersed separately in methanol (1:3 w/v) solvents for overnight. The experiment in this study used the dried extract of cuttlefish ink. The isolate of A. hydrophila was originated from Jepara Brackishwater Aquaculture Center. The average yield percentage of cuttlefish ink extract obtained was 4.86%. The results of the MIC test in Table V show that the highest average absorbance value obtained was at a concentration of 50 ppm, which was equal to 1.716 nm, and the lowest absorbance was obtained at a treatment dose of 300 ppm at 0.841 nm, while the Mc Farland tube was 0.933 nm. The results of antibacterial test on Table II showed antibacterial activity of cuttlefish ink extract at negative concentration control showed diameter zone of 5 ± 1.2 mm, at positive control showed diameter zone of 31 ± 1.2 mm, at 250 ppm result 19 ± 0.9 mm, at 300 ppm result 22 ± 1.4 mm, at 350 ppm result 31 ± 1.2 mm.


A. hydrophila; Antibacterial; Cuttlefish Ink; Extract; Sepia sp.

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Allsop, A.E., 1998). ‘New antibiotic discovery, novel screens, novel targets. and impact of microbial genomics’. Curr opinions. Microbiol. 1, 530-534.

Annaian S., Thangaraj A., Chendur P. G. D., and Thangavel B. (2008). Antimicrobial activity of sulphated mucopolysaccharides (Heparin and heparine like Glycosaminoglycans (GAGs)) from cuttlefish, Euprymna berryi Sasaki, 1929. Trends in Applied Science Research. 3, 97-102.

Barker, G., 2001, Bacterial diseases. In: BSAVA Manual of Ornamental Fish, 2nd Edition (Wildgoose, W.H., ed.), UK.

Bayne, C.J., 1973, Internal defense mechanisms of Octopus dolfeini. Malacol. Rev. 6, 13–17.

Caldwell, R. L. (2005). An observation of inking behavior protecting adult Octopus bocki from predation by green turtle (Chelonia mydas) hatchlings. Pac. Sci., 2005; 59: pp. 69-72.

Derby, C.D., Kicklighter, C.E., Johnson, P.M., and Zhang, X. (2007). Chemical composition of inks of diverse marine mollusks suggests convergent chemical defenses. J. Chem. Ecol, 2007; 33(5): pp. 1105- 1113.

Fadjar, M., Andajani, S., & Zaelani, K. (2016). Squid (Loligo edulis) ink raw extract as an anti-vibriosis substance in grouper (Epinephelus fuscoguttatus) juvenile culture infected by Vibrio alginolyticus. AACL Bioflux, 2016; 9(2): pp. 422-428.

Fitrial, Y., & Khotimah, I. K. (2017). Antibacterial Activity of Melanin from Cuttlefish and Squid Ink. Jurnal Pengolahan Hasil Perikanan Indonesia, 20(2), 266.

Hanlon, R.T. & Messenger J.B., 1996, Cephalopod behavior. Cambridge University Press, Cambridge. 1996. pp. 232.

Kicklighter, C.E. & Derby, C. D. (2006). Multiple components in ink of the sea hare Aplysia californica are aversive to the sea anemone Anthopleura sola. J. Exp. Mar. Biol. Ecol, 2006; 334: pp. 256 -268.

Lane F.W., 1962, Economic. In: Kingdom of the octopus, the worlds of science (ecology) pyramid publication, New York, 181-201.

Mochizuki, A., 1979, ‘An antisepic effect of cuttle fish ink’. Bull. Jap. Soc. Sci. Fish., 11, 1401 -14.

Neifar A, Rebah FB, Gargouri AF, Abdelmouleh A. 2009. Physicochemical characterization of Sepia officinalis ink and the effects of storage conditions on the coagulation process. Journal of the Marine Biological Association of the UK. 89(04): 803-807.

Nithya M, Ambikapathy V., and Panneerselvam A., (2011). Effect of pharaoh’s cuttlefish ink against bacterial pathogens. Asian Journal of Plant Science Research. 49: pp. 49-55.

Nkere, C.K., and Iroegbu, C. (2005)., Antibacterial screening of the root, stem and stembark extracts of Picralima nitida., Afri J of Biotech. 4 (6) (2005) pp. 522 – 526.

Norman, M.D., 2000. Cephalopods. A World Guide. Conch Books, Hackenheim, Germany 2000, pp. 172.

Ortonne J.P., Voulot C., Khatchadourian C., Palumbo A., and Prota G. 1981). A reexamination of melanogenesis in the ink gland of cephalopods. In: Pigment cell. Phenotypic Expression in Pigment cells M.Seiji. Ed.) University of Tokyo Press, Tokyo. 1981. pp. 49-57.

Patil, R. G. J., Shanmugam S.A., & Shakila J.R., 2001. ‘Control of bacterial pa-thogens, associated with fish diseases, by antagonistic marine actinomycetes iso-lated from marine sediments’. Indian J. Mar. Sci. 1, 264–267.

Rahman, M.M., Wahed, M.I.I., Biswas, M.H., Sadik, G.M. & Haque, M.E., 2001. ‘In Vitro Antimicrobial Activity of the Compounds of Trapa Bispinasa Roxb’, Sci 1, 214-216.

Rajaganapathi J., Thyagarajan S.P., and Patterson Edward J.K., 2000, Study on cephalopod’s ink for antiretroviral activity. Indian Journal of Experimental Biology. 2000. 36: pp. 583-587.

Ramaswamy P., Subhapradha N., Srinivasan A., Shanamugam V., Krishnamoothy J., and Shanmugam A. (2011). In vitro evaluation of the antimicrobial activity of methanolic extract from selected species of cephalopods on clinical isolates. African Journal of Microbiology research, 2011.5: pp. 3884-3889.

Ramaswamy P., Vino A.B., Saravanan R., Subhapradha N., Shanamugam V. and Shanmugam A. (2011). Screening of antimicrobial potential of polysaccharide from cut Lane FW (1962). Economic.In: Kingdom of the octopus, the worlds of science (ecology) pyramid publication, New York, pp. 181-201tlebone and methanolic extract from body tissue of Sepia prashadi Winkworth, 1936. Asian Pacefic Journal of Tropical Biomedicine, 2011, pp 244-248.

Rios, J.L., Recio, M.C., and Villar, A. (1988). Screening methods for natural products with antimicrobial activity: a review of the literature. J Ethnopharmacol leaflets 23 (1988) pp. 127-149.

Roberts, R.J., Frerichs, G.N. & Miller, S.D., 1992, In: Diseases in Asian Aquaculture. I. Fish Health Section. Edited by Shariff M., Subasinghe R.P., and Arthur J.R., Asian Fish. Soc., Manila, 1992, pp. 431-436.

Shanmugam A, T.S Mahalekshmi, & Barwin Vino A., 2008, Antimicrobial activity of Polysaccharide Isolated from the Cuttlebone of Sepia aculeate and Sepia brevimana. An Approach to selected antimicrobial activity for human pathogenic microorganisms. Journal of fisheries and aquatic science, 2, 268-274.

Sheu T.Y., & Chou. C. C., 1990, ‘Antimicrobial activity of squid ink’. J. Chin. Agric. Chem. Soc., 28: pp. 59-68.

Takai M., Kawai Y., Inoue N. & Shinano H., 1992, ‘Comparative studies on microbiological andchemical characteristics of Ika-Shiokaraakazukuri and Ika- Shiokara Kurozukuri’. Bull. Jpn Soc. Sci. Fish. 58, 2373 – 2378.

Vasantharaja D, Ravitchandirane V and Anandan V. 2014. Anti-microbial activity and spectro-chemical investigation of ink extracts of Sepiella inermis (Van Hasselt 1835). Notulae Scientia Biologicae. 6(3):273-275.

Vennila R., Rajesh Kumar R.K., Kanchana S., Arumugam M., & Balasubramanian T. 2011, Investigation of antimicrobial and plasma coagulation property of some molluscan ink extracts: Gastropods and cephalopods. African Journal of Biochemistry and Research. 5, 14-21.

Voss, G.L., 1973, Cephalopod resources of the world. FAO Fisheries Circular 149: 75.

Walters, E.T., and Erickson, M.T. (1986). Directional control and the functional organization of defensive responses in aplysia. J. Comp. Physiol. A., 1986; 159: pp. 339-351.

Yuvaraj D, Suvasini B, Chellathai T, Fouziya R, Ivo Romauld S and Chandran M. 2015. Anti-bacterial studies on the difference body parts of Loligo duvauceli. Journal of Chemical and Pharmaceutical Research. 7(6):406-408.

Zainab D. D., & Ali Tahar A., 2010. Antimicrobial activity of some crude marine Mollusca extracts against some human pathogenic bacteria. Thi-Qar Medical Journal (TQMJ). 4, 142-147.

Zhong, Guang W., Jiang-Hua S., Jiang-Qiu P., Kun Li, Yan H., & Hua-Zhong L., 2009, ‘Protective effects of Squid ink extract towards hemopoietic injuries induced by cyclophosphamide’. Mar. Drugs., 1, 9-18.


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