ANALGESIC EFFECT OF COMBINATION OF ESSENTIAL OIL Curcuma xanthorriza Roxb. AND ITS CURCUMINOIDS IN MICE

Abstract

The study was conducted to determine the analgesic effect of the essential oil of ginger rhizome (Curcuma xanthorriza Roxb.), Kurkuminoid, and a combination of both. Extracting of kurkuminoid performed with ethyl acetate and hexane solvent. Essential oil obtained from the water-steam distillation. Analgesic test was conducted using formalin test (20μL 1% formalin, intraplantar) against the Swiss strain male mice (30±5g), age 2 months. Test group includes 0.5% CMC-Na P.O. (Negative control), indomethacin 4mg/kg P.O. and i.p. (Positive control), kurkuminoid 48mg/kg po, essential oil 24mg/kg po, po and their combinations Formalin injection was performed 1 hour after sample treatment. Data licking time taken in Phase I (0-5min) and phase II (10-30min) after formalin injection. Phase I indicate pain that occurs in the central nervous system (CNS), while phase II in the peripheral area. The results of the phase I trials show analgesic power of indomethacin po (4.96±3.26%) and ip (4.54±1.65%). Kurkuminoid and each essential oil (1.22±0.89%) and (28.8±8.48%), while the mixture (42.14±5.6%). Kurniawan (2007) reported that the magnitude of phase I of the power analgesic morphine 5mg/kg po was (69.68±2.17%). In phase II of essential oils have analgesic power of (74.63±1.97%), a mixture of essential oil (24mg/kg) and kurkuminoid (48mg/kg) has analgesic power of (67.56±0.59% ), indomethacin ip (49.46 ± 1.08%) and po (49.82 ± 0.91%), and kurkuminoid (44.80±1.46%).