CYTOTOXICITY STUDY OF MEZZETIAPARVIFLORABECC. WOODBARK

https://doi.org/10.22146/tradmedj.7758

Mufidah Mufidah(1*), Elly Wahyudin(2), Gatot S. Lawrence(3), M. Sri Hartati Wahyuningsih(4), Mirianti A. Manggau(5)

(1) Faculty of Pharmacy, Hasanuddin University
(2) Faculty of Pharmacy, Hasanuddin University
(3) Faculty of Medicine, Hasanuddin University
(4) Faculty of Medicine, Universitas Gadjah Mada
(5) Faculty of Pharmacy, Hasanuddin University
(*) Corresponding Author

Abstract


The  cytotoxic activity  of acetone insoluble extract of MezzetiaparvifloraBecc. Wood bark was evaluated on HeLa cell line and it was compared with normal vero cell line to confirming the use of the plant as a traditional medicine for tumor. The experiment with normal vero cell line using MTT assay showed a percentage of cell  viability of  96.8%  at1000 µg of concentration which was not increase with the increase of  concentration of the extract. Whereas, the experiment on HeLa cell line showed a low cytotoxic activity with the viability percentage was found to be 87.4% at1000μg/ml. Therefore the extract was categorized as non-toxic and the next studies is necessary to explore the mechanism liable of the using  of the plant extract as anticancer traditional.


Keywords


Mezzetiaparviflora Becc., MTT, Cytotoxicity, Vero cell line, HeLa cell line

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References

Cui, B., Chai, H., Santisuk, T., Reutrakul, V., Fransworth, N. R., Corell, G. A., Pezzuto, J.M., Kinghorn, A. D. Novel Cytotoxic Acylated Oli gorhamno sides from Mezzettialeptopoda J. Nat. Prod. 1998.61(12): 1535-1538

Middleton, Jr E.,Kandaswami,C.,Theoharides,T.C. The effects of plant flavonoids on mammalian cells : implications for inflammation, heart disease, and cancer. Pharmacol Rev2000;52:673–751

Murdifin, M.,Wahyudin, E., Lawrence, G.S., Subehan,Manggau, M.A., and Alam, G. 2012.Phytochemical analysis and antioxidant activity of MezzetiaparvifloraBecc.Woodbark extract. Pharmacognosy Journal, 2012; 4(34):18-21

Pour, B.M., Latha, L.Y. and Sasidharan, S. 2011. Cytotoxicity and Oral Acute Toxicity Studies of Lantana camaraLeaf Extract.Molecules 16: 3663-3674

Sosef, M.S.M., Hong,L.T., dan Prawirohatmodjo, S. (Editor). 1998. PROSEA (Plant Resources of South East asia) Jilid 5(3); Timber Tree; lesser known timber. Prosea Foundation Indonesia. 374-375

Wills J.C. (revd. Airy Shaw HK) A Dictionary of the Flowering Plants and Ferns, 8th Ed.; University Press: Cambridge, UK, 1973: 739

Yang,C.S., Landau, J.M., Huang,M.T., Newmark,H.L. Inhibition of carcinogenesis by dietary poly phenolic compounds. AnnuRevNutr2001;21:381–406

Yang, C.S., Maliakal, P., Meng, X. Inhibition of carcino-genesis by tea. Annu Rev Pharmacol Toxicol. 2002. 42:25–54 in Jeong, W.S. and Kong, A.T. Biological Properties of Monomeric and Polymeric Catechins: Green Tea Catechins and Procyanidins. Pharmaceutical Biology 2004,Vol.42, Supplement, pp.84–93



DOI: https://doi.org/10.22146/tradmedj.7758

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