AWAR–AWAR (Ficus septica Burm. f.) LEAVES ETHANOLIC EXTRACT INDUCED APOPTOSIS OF MCF-7 CELLS BY DOWNREGULATION OF Bcl-2

https://doi.org/10.22146/tradmedj.8133

Dewi Arum Sekti(1), Muhammad Fithrul Mubarok(2), Inna Armandani(3), Sendy Junedy(4), Edy Meiyanto(5*)

(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Pharmacy, Universitas Gadjah Mada
(4) Faculty of Pharmacy, Universitas Gadjah Mada
(5) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Alkaloid fenantroindolisidin showed cytotoxic activity against several cancer cells. Ethanolic leaf extract of Awar-awar proven to increase the cytotoxic activity of chemotherapy agent doxorubicin in MCF-7 cells. This study aims to determine the effect of Ethanolic leaf extract of Awar-awar in apoptosis induction of breast cancer cells MCF-7.Awar-awar leaf powder was extracted by maceration using 70% ethanol and then concentrated to obtain concentrated of Awar-awar. Apoptosis induction was examined by double staining method and the expression of Bcl-2 protein was observed by  immunohistochemistry method. Single treatment of Awar-awar leaf extract  showed the ability of extracts to induce apoptosis and downregulated the expression of Bcl-2 protein. Ethanolic leaf extract of Awar-awar is potential as co-chemotherapeutic agent.

Keywords


Awar-awar (F. septica Burm. f.), MCF-7 cells, apoptosis, Bcl-2



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DOI: https://doi.org/10.22146/tradmedj.8133

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