Novena Yety Lindawati(1), Agung Endro Nugroho(2*), Suwijiyo Pramono(3)

(1) Academy of National Pharmacy, Surakarta
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author


Sambiloto herb (Andrographis paniculata (Burm. f.) Nees.) and pegagan herb (Centella asiatica (L.) Urban) are excellent medicinal plants which are being developed as traditional for antidiabetic. The research aims to see the effect of combination from purified extract of sambiloto herb and pegagan herb of translocation of GLUT-4 protein in model type 2 diabetes mellitus rats-insulin resistance compared with the use of each purified extract. Test preparations were divided into 8 groups include three from combination purified extract of sambiloto herb and pegagan herb: I (912,1: 300), II (651,5: 500), III (390,9: 700) in mg/kg BW, IV (purified extract of sambiloto herb 1303 mg/kg BW), V (purified extract of pegagan herb 1000 mg/kg BW), VI (positive control metformin 45 mg/kg BW), VII (negative control CMC-Na 0.5%) and VIII (normal control). Parameters measured semi quantitative data translocation of GLUT-4 protein in cells muscles of the thigh (soleus muscle) rats using Immunohistochemistry methods. Test animals are of type 2 diabetes mellitus insulin-resistance was induced by fructose (1,8 g/kg BW) and fat-rich food (95% pellet, 5% egg yolks and 5 mL/ 200 g BW lard) for 70 days. Determination of insulin resistance used 3 parameters such as blood glucose levels, lipid levels (triglycerides, LDL, cholesterol, and HDL), and hypoglikemic tests with glibenklamid compared to normal controls animals. Insulin resistance test results using statistical analysis Independent Samples t-Test showed significant differences (p< 0.05) between normal rats with fat-fructose rats at day-50 and -70. This indicate fat-fructose rats indicated type 2 DM insulin resistance. Group of combination I (combination purified extract of sambiloto herb and pegagan herb (912,1: 300 mg/kg BB) showed effect of translocation of GLUT-4 protein was better than each purified extract with significantly p<0.05 with oneway ANOVA.


Andrographis paniculata, Centella asiatica, GLUT-4 protein


Ahmad, M., Razak, A., Akowuah, G. A., Asmawi, Z., Zhari, I., 2007, HPLC profile and antihyperglycemic effect of ethanol extracts of Andrographis paniculata in normal and streptozotocin-induced diabetic rats, J Nat Med, (61) : 422–429

Akbar, S., 2011, Andrographis paniculata: A Review of Pharmacological Activities and Clinical Effect, Alternative Medicine Review, 16 (1) : 66-77

Anonim, 2007, Centella asiatica L., Alternative Medicine Review, 12 (1) : 69-74

Brinkhaus, B., Lindner, M., Schuppan, D., and Hahn, E. G., 2000, Chemical, pharmacological and clinical profile of the East Asian medical plant Centella asiatica, Phytomedicine, 7 (5) : pp. 427-448

Chauhan, P.K., Pandey, I.P., Dhatwalia, V.K., and Singh, 2010, Anti-Diabetic Effect of Ethanolic and Methanolic Leaves Extract of Centella asiatica on Alloxan induced Diabetic Rats, International Journal of Pharma and Bio sciences, VI (2) : 1-6

Choi, C. S., Lee, F. N., Youn, J. H., 2001, Free Fatty Acids Induced Peripheral Insulin Resistance without Increasing Muscle Hexosamine Pathway Product Level in Rats, Diabetes, (50) : 418-424

Gayathri, V., Lekshmi, P., Padmanabhan, R.N., 2011, Anti-diabetes activity of ethanol extrac of Centella asiatica L., Urban (Whole plant) in Streptozotocin-Induced Diabetic Rats, Isolation of active Fraction and Toxicity evaluation of The Extrac, Int. J. Med. Arom. Plants, ISSN 2249-4340, 1 (3) : 278-286

Hosain, A. Md., Roy, B. K., Ahmed, K., Chowdhury, S. A. M., and Rashid, M. A., 2007, Antidiabetic Activity of Andrographis paniculata, Dhaka Univ. J. Pharm. Sci., 6 (1) : 15-20

Jamil, S. S., Nizami, Q., and Salam, M., 2007, Centella asiatica (Linn.) Urban: A Review, Natural Product Radiance, 6 (2) : pp. 158-170.

Lauritzen, H. P., Schertzer, J. D., 2010, Measuring GLUT 4 Translocation in Mature Muscle Fibers, Am J Physiol Endocrinol Metab., 299 (2) : E 169-79

Niranjan, A., Tewari, S. K., Lehri, A., 2010, Biological Activities of Kalmegh (Andrographis paniculata Nees) and Its active principles-A Review, Indian Journal of Natural Products and Resources, 1(2) : 125-135

Nugroho, A.E., Andrie, M., Warditiani, N.K., Siswanto, E., Pramono, S., A Ekstrak terpurifikasi and Lukitaningsih E., 2012, Antidiabetic and antihiperlipidemic effect of Andrographis paniculata (Burm. f) Nees and Andrographolide in High-Fruktose-Fat-Fed Rats, Indian J Pharmacol, 44 (3) : 377-381

Nugroho, A.E., Lindawati, N.Y., Herliyanti, K., Widyastuti, L., Pramono, S., 2013, Anti-diabetic effect of a combination of andrographolide-enriched extract of A. paniculata and asiaticoside-enriched extract of Centella asiatica L. in high-fructose-fat-fed rats, Indian Journal of Experimental Biology, 51 : 1101-1108

Paocharoen, V., 2010, The Efficacy and Side Effects of Oral Centella asiatica extract for Wound Healing Promotion in Diabetic Wound Patients, J Med Assoc Thai, 93 (Suppl. 7) : S166-S170

Qin, B., Nagasaki, M., Ren, M., 2004, Cinnamon Extrac Prevents The Insulin Resistance Induced by High-Fructose Diet, Horm. Metab. Res., 36 : 119-12

Subramanian, R., 2009, Effect of Ethanolic Extracts of Andrograpis paniculata on Type 2 Diabetes Mellitus and Insulin Resistant Rats, Desertation, Universiti Sains Malaysia, Malaysia

Subramanian, R., Azmawi, M. Z., Sadikun, A., 2008, In vitro a-glucosidase and a-amylase enzyme inhibitory effects of Andrographis paniculata extract and andrografolid, Acta Biochimica Polonia, 55 (2) : hal 391-398

Thipkaew, C., Wattanathorn, J., and Muchimapura, S., 2012, The Beneficial effect of Asiaticoside on Experimental Neuropathy in Diabetic Rat, American Journal of Applied Sciences, 9 (11) : 1782-1788

Vohra, K., Pal, G., Gupta, V. K., Singh, S., and Bansal, Y., 2011, An Insight on Centella asiatica Linn.: A Review on Recent Reseach, Pharmacologyonline, (2) : 440-462

Widyawati, T., 2007, Aspek Farmakologi Sambiloto (Andrograpis paniculata Nees), Majalah Kedokteran Nusantara, (40) : 3

Yu, B. C., 2008, Chang, C. K., Su, C. F., and Cheng, j. T., Mediation of β-endorphin in andrographolide-induced plasma glucose-lowering action in type I diabetes-like animals, Naunyn-Schmiedeberg’s Arch Pharmacol, (377) : 529–540

Yulinah, E., Sukrasno, Fitri, M. N., 2001, Aktivitas Antidiabetik Ekstrak Etanol Herba Sambiloto (Andrograpis paniculata Nees (Acanthaceae)), JMS, 6 (1) : 13-20

Zhang, Z., Jiang, J., Yu, P., Zeng, X., Larrick, J.W., Wan, Y. 2009, Hypoglycemic and beta cell protective effects of andrographolide analogue for diabetes treatment, J. Transl. Med, (7) : 62-69

Zavaroni, I., Sander, S., Scott, S., Reaven, G. M., 1980, Effect of Fructose Feeding on Insulin Secretion and Insulin Action in The Rat, Metabolism, 29 (10) : 970-983


Article Metrics

Abstract views : 2819 | views : 6336


  • There are currently no refbacks.

Copyright (c) 2015 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada