Anti-inflammatory Effects of Channa micropeltes Extract through NF-κB and TNF-α in Diabetic Rat

Amy Nindia Carabelly(1), Didit Aspriyanto(2), Milka Widya Sari(3), Erika Norfitriyah(4), Juliyatin Putri Utami(5*)

(1) Department of Oral Pathology and Maxillofacial, Faculty of Dentistry, University of Lambung Mangkurat, Banjarmasin, South Kalimantan
(2) Department of Oral Biology Faculty of Dentistry, University of Lambung Mangkurat, Banjarmasin, South Kalimantan
(3) Faculty of Dentistry, University of Lambung Mangkurat, Banjarmasin, South Kalimantan
(4) Department of Pediatric Dentistry Faculty of Dentistry, University of Lambung Mangkurat, Banjarmasin, South Kalimantan
(5) Department of Biomedicine Faculty of Dentistry, University of Lambung Mangkurat, Banjarmasin, South Kalimantan
(*) Corresponding Author


Diabetes mellitus (DM) is a disorder of carbohydrate, fat, and protein metabolism caused by impaired insulin secretion, insulin action, or both characterized by hyperglycemia. Hyperglycemic condition is associated with inflammation process leading to complications. Food supplements play an important role in controlling protein metabolism and have anti-inflammatory properties such as Channa micropeltes (CM). This study aimed to analyze the effect of CM extract on the levels of molecular markers Nuclear factor kappa beta (NF-κB) and Tumor Necrosis Factor alfa (TNF-α) in normal and diabetic rats. The experimental design of this investigation was a post-test-only control group design and the samples were the diabetic model group given CM extract at 16 mL/kgBB dose, and negative control group for 14 days, respectively. Serum concentrations of    NF-κB and TNF-α were measured by ELISA. DM-CM group showed decreasing levels of NF-κB. TNF- α was detected with the lowest level in the N-CM group. The levels of TNF- α declined close to normal levels in the DM-CM group. Hence, the conclusion was the CM at a dose of 16 mL/kg BW for 14 days in Wistar rats can reduce Nf-κB and TNF-α in groups of normal and diabetic rats.


cytokines; diabetes; Nf-κB; TNF- α; traditional medicine

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Ajiboye, B.O., Ojo, O.A., Akuboh, O.S., Abiola, O.M., Idowu, O. & Amuzat, A.O. (2018). Anti-hyperglycemic and anti-inflammatory activities of polyphenolic-rich extract of syzygium cumini linn leaves in alloxan-induced diabetic rats. Journal of Evidence-Based Integrative Medicine, 23.

Alauddin, A., Andrie, M. & Purwanti, N.U. (2016). Uji Efek Ekstrak Ikan Gabus (Channa striaa) pada Luka Sayat dengan Tikus Putih Jantan Galur WIstar yang diberikan secara oral. Journal of Knowledge Management, 2(2).

Alzamil, H. (2020). Elevated Serum TNF- α Is Related to Obesity in Type 2 Diabetes Mellitus and Is Associated with Glycemic Control and Insulin Resistance. Journal of Obesity, 2020.

Ambrozova, N., Ulrichova, J. & Galandakova, A. (2017). Models for the study of skin wound healing. The role of Nrf2 and NF-κB. Biomedical Papers, 161(1), 1–13.

Apriasari, M.L., Ainah, Y., Febrianty, E. & Carabelly, A.N. (2018). Antioxidant Effect of Channa Micropeltes in Diabetic Wound of Oral Mucosa. International Journal of Pharmacology, 15(1).

Apriasari, M.L., Syahadati, M.A. & Carabelly, A.N. (2020). Clinical Analysis of Channa micropeltes for Treating Wound of Diabetes Mellitus. Berkala Kedokteran, 16(1).

Beserra, F.P., Gushiken, L.F.S., Vieira, A.J., Bérgamo, D.A., Bérgamo, P.L., Souza, M.O. de, Hussni, C.A., Takahira, R.K., Nóbrega, R.H., Martinez, E.R.M., Jackson, C.J., Maia, G.L. de A., Rozza, A.L. & Pellizzon, C.H. (2020). From inflammation to cutaneous repair: Topical application of lupeol improves skin wound healing in rats by modulating the cytokine levels, NF-κB, Ki-67, growth factor expression, and distribution of collagen fibers. International Journal of Molecular Sciences, 21(14).

Cao, Y., Liu, X., Li, Y., Lu, Y., Zhong, H., Jiang, W., Chen, A.F., Billiar, T.R., Yuan, H. & Cai, J. (2017). Cathepsin L activity correlates with proteinuria in chronic kidney disease in humans. International Urology and Nephrology, 49(8).

Chandrasekharan JA, Sharma-Walia N. (2015). Lipoxins: nature's way to resolve inflammation. J Inflamm Res, 30;8:181-92. doi: 10.2147/JIR.S90380. PMID: 26457057; PMCID: PMC4598198.

Chawla, A., Chawla, R. & Jaggi, S. (2016), Microvasular and macrovascular complications in diabetes mellitus: Distinct or continuum?. Indian Journal of Endocrinology and Metabolism, 20(4), 546–553.

He, F., Wu, C., Li, P., Li, N., Zhang, D., Zhu, Q., Ren, W. & Peng, Y. (2018). Functions and Signaling Pathways of Amino Acids in Intestinal Inflammation. BioMed Research International, 2018.

Kaur, P. & Choudhury, D. (2020). Modulation of Inflammatory Dynamics by Insulin to Promote Wound Recovery of Diabetic Ulcers. Wound Healing.

Liu, T., Zhang, L., Joo, D. & Sun, S.C. (2017), NF-κB signaling in inflammation, Signal Transduction and Targeted Therapy, 2.

Marion-Letellier, R., Savoye, G. & Ghosh, S. (2015). Polyunsaturated fatty acids and inflammation, IUBMB Life, 67(9).

Omar, M.N., Yusoff, N.S.A.M., Zainuddin, N.A. & Yunus, K. (2010). ω-Fatty acids from Malaysian giant snakehead (Channa micropeltes) fish oil. Oriental Journal of Chemistry, 26(1).

Oscarsson J., Hurt-Camejo E. (2017). Omega-3 fatty acids eicosapentaenoic acid and docosahexaenoic acid and their mechanisms of action on apolipoprotein B-containing lipoproteins in humans: a review. Lipids Health Dis, 10;16(1):149. doi: 10.1186/s12944-017-0541-3.

Piero, M.N., G.M. Nzaro, J.M. Njagi. (2014). Diabetes mellitus – a devastating metabolic disorder, Asian Journal of Biomedical and Pharmaceutical Sciences, 04 (40) ,1-7.

Portou, M.J., Yu, R., Baker, D., Xu, S., Abraham, D. & Tsui, J. (2020), Hyperglycaemia and Ischaemia Impair Wound Healing via Toll-like Receptor 4 Pathway Activation in vitro and in an Experimental Murine Model, European Journal of Vascular and Endovascular Surgery, 59(1), 117–127.

Ren, W., Liao, Y., Ding, X., Jiang, Y., Yan, J., Xia, Y., Tan, B., Lin, Z., Duan, J., Jia, X., Yang, G., Deng, J., Zhu, C., Hardwidge, P.R., Li, J., Zhu, G. & Yin, Y. (2019), Slc6a13 deficiency promotes Th17 responses during intestinal bacterial infection. Mucosal Immunology, 12(2).

Simonetto, Marialaura, Marco Infante, Ralph L. Sacco, Tatjana Rundek, and David Della-Morte, (2019). A Novel Anti-Inflammatory Role of Omega-3 PUFAs in Prevention and Treatment of Atherosclerosis and Vascular Cognitive Impairment and Dementia, Nutrients 11, no. 10: 2279.

Singh, V.P., Bali, A., Singh, N. & Jaggi, A.S. (2014). Advanced glycation end products and diabetic complications, Korean Journal of Physiology and Pharmacology, 18(1).

Suryavanshi, S. V. & Kulkarni, Y.A. (2017). NF-κβ: A potential target in the management of vascular complications of diabetes, Frontiers in Pharmacology, 8(NOV).


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