Identification of Myostatin Gene Polymorphism (MSTN|Taq1) Exon-2 in Pitalah Duck Using The PCR-RFLP Method

Kusnadidi Subekti; Winanti Salsabila; Nada Iswari Samara; Firda Arlina

doi:10.21059/buletinpeternak.v49i3.106398

Identification of Myostatin Gene Polymorphism (MSTN|Taq1) Exon-2 in Pitalah Duck Using The PCR-RFLP Method

https://doi.org/10.21059/buletinpeternak.v49i3.106398

Kusnadidi Subekti^(1*), Winanti Salsabila⁽²⁾, Nada Iswari Samara⁽³⁾, Firda Arlina⁽⁴⁾

(1) Department of Science, Technology and Livestock Production, Faculty of Animal Science, Universitas Andalas, Padang 25163
(2) Student of Faculty of Animal Science, Universitas Andalas, Padang 25163
(3) Student of Faculty of Animal Science, Universitas Andalas, Padang 25163
(4) Department of Science, Technology and Livestock Production, Faculty of Animal Science, Universitas Andalas, Padang 25163
(*) Corresponding Author

Abstract

This study aimed to identify the polymorphism of the exon-2 myostatin gene (MSTN) in Pitalah ducks using the PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) method. 28 blood samples of Pitalah ducks were used in this study. Blood samples were extracted using Intron Biotechnology's Intron G-SpinTM Total DNA Extraction Kit protocol. The DNA extraction results were then amplified using a pair of primers, namely the Forward: 5' TCCACTTGTTACTGATGCTGT-3' primer and the Reverse: 5'- TAGGGAAATGGAGGCACAGG-3' primer, with a fragment target of 700 bp. Furthermore, the amplified product is restricted using the Taq1 enzyme, which recognizes the cutting site on T|CGA. Based on the results of the study, two genotypes were found, namely 89.29% truncated homozygous (+/+) and heterozygous (+/-), as well as 10.71% of the total sample used, while untruncated homozygous genotypes (-/-) were not found in this study. Meanwhile, the allele frequency (+) was 0.946, the allele frequency (-) was 0.054, and the observed heterozygosity value was greater than the expected heterozygosity value (Ho>He). This study concludes that the myostatin exon-2 gene in Pitalah ducks is polymorphic and is in Hardy-Weinberg equilibrium.

Keywords

Genotyping, Myostatin gene, PCR-RFLP, Pitalah duck, Polymorphism, Taq1 enzyme

Full Text:

1. Kusnadidi Subekti 164-171

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DOI: https://doi.org/10.21059/buletinpeternak.v49i3.106398