* Corresponding Author
Comparative Effects of Selenium and Gonadotropin Supplementation on In Vitro Maturation of Bligon Goat Oocytes
https://doi.org/10.21059/buletinpeternak.v50i2.115278 Arina Dinar Nurfadilla Sastranegara(1), Rona Agustina(2), Dio Fico Felsidan Diatmono(3), Fransisca Gani Padmawati(4), Pradita Iustitia Sitaresmi(5), Sigit Bintara(6), Diah Tri Widayati(7*)
(1) Laboratory of Animal Physiology and Reproduction, Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, 55281 Sleman, Yogyakarta, Indonesia
(2) Laboratory of Animal Physiology and Reproduction, Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, 55281 Sleman, Yogyakarta, Indonesia
(3) Laboratory of Animal Physiology and Reproduction, Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, 55281 Sleman, Yogyakarta, Indonesia
(4) Laboratory of Animal Physiology and Reproduction, Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, 55281 Sleman, Yogyakarta, Indonesia
(5) Research Center for Animal Husbandry, Cibinong Science Center, National Research and Innovation Agency (BRIN), 16915 Bogor, Jawa Barat, Indonesia
(6) Laboratory of Animal Physiology and Reproduction, Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, 55281 Sleman, Yogyakarta, Indonesia
(7) Laboratory of Animal Physiology and Reproduction, Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, 55281 Sleman, Yogyakarta, Indonesia
(*) Corresponding Author
Abstract
Selenium (Se) is a novel addition to in vitro maturation (IVM) media, unlike the traditional gonadotropin hormone. This highlights the advancements in reproductive biology and potential strategies to enhance oocyte maturation. This research was conducted to determine the effect of Se and gonadotropin hormone supplementation into maturation medium on the in vitro oocyte maturation of Bligon goat. Ovaries from Bligon goats were obtained from a slaughterhouse in Special Region of Yogyakarta. High-quality oocytes (grades A and B) were retrieved through aspiration, washed, and cultured in medium beneath mineral oil. Three experimental conditions were established: 50 μL of unsupplemented medium (T0), 48 μL of medium + 2 μL of gonadotropin (T1), and 48 μL of medium + 2 μL of Se (T2). The culture process was conducted for 24 h at 39°C, 95% humidity, and 5% CO2. Oocyte maturation was evaluated based on cumulus cell expansion, and the data obtained were analyzed using One-Way ANOVA, followed by Tukey’s Post Hoc test. The results indicated that the inclusion of either Se (86.26±7.20%) or gonadotropin (88.13±7.85%) in the IVM medium significantly enhanced (p<0.05) the maturation rate compared with that of the unsupplemented group (50.09±11.90%). However, there was no significant difference (p>0.05) between the two supplements. In conclusion, both Se and gonadotropin hormones proved to be effective supplements for improving the maturation rate of Bligon goat oocytes.
Keywords
Full Text:
4. Arina Sastranegara PDFReferences
Ashibe, S., K. Irisawa, K. Yokawa, and Y. Nagao. 2021. Mechanism of the adverse effect of hyaluronidase used for oocyte denudation on early development of bovine embryos. Zygote. 29:337–341. https://doi.org/10.1017/S0967199421000010.
Baldini, G. M., D. Lot, A. Malvasi, A. S. Laganà, A. Vimercati, M. Dellino, E. Cicinelli, D. Baldini, and G. Trojano. 2024. Abnormalities of oocyte maturation: mechanisms and implications. IJMS. 25:12197. https://doi.org/10.3390/ijms252212197.
Chang, T., J. Zhao, Q. Li, A. Meng, Q. Xia, Y. Li, W. Xiang, and Z. Yao. 2023. Nuclear-cytoplasmic asynchrony in oocyte maturation caused by TUBB8 variants via impairing microtubule function: a novel pathogenic mechanism. Reprod Biol Endocrinol. 21:109. https://doi.org/10.1186/s12958-023-01161-y.
Dahlen, C. R., L. P. Reynolds, and J. S. Caton. 2022. Selenium supplementation and pregnancy outcomes. Front. Nutr. 9:1011850. https://doi.org/10.3389/fnut.2022.1011850.
Di Nisio, V., S. Antonouli, S. Colafarina, O. Zarivi, G. Rossi, S. Cecconi, and A. M. G. Poma. 2023. Repeated rounds of gonadotropin stimulation induce imbalance in the antioxidant machinery and activation of pro-survival proteins in mouse oviducts. IJMS. 24:9294. https://doi.org/10.3390/ijms24119294.
Ferro, C., H. F. Florindo, and H. A. Santos. 2021. Selenium nanoparticles for biomedical applications: from development and characterization to therapeutics. Adv Healthcare Materials. 10:2100598. https://doi.org/10.1002/adhm.202100598.
Guo, H., T. Yang, C. Xia, Z. Ni, Y. Lou, H. Deng, Z. Zuo, Y. Zhu, X. Wang, and J. Fang. 2026. Glutathione selenium nanoparticles alleviate oxidative stress-induced reproductive dysfunction by regulating mitochondrial dynamics. Free Radical Biology and Medicine. 242:108–119.https://doi.org/10.1016/j.freeradbiomed.2025.10.277.
Guseva, O., N. Kan, V. Chekmareva, D. Kokorev, and P. Ilyasov. 2024. The impact of antioxidant supplements on oocytes and preimplantation embryos of humans and mammals, and their potential application for mitigating the consequences of oxidative stress in vitro: a review. Reproductive and Developmental Medicine. 8:252–263. https://doi.org/10.1097/RD9.0000000000000100.
Jamil, M., H. Debbarh, A. Kabit, M. Ennaji, M. Zarqaoui, W. R. Senhaji, M. Hissane, B. Saadani, N. Louanjli, and R. Cadi. 2023. Impact of the number of retrieved oocytes on IVF outcomes: oocyte maturation, fertilization, embryo quality and implantation rate. Zygote. 31:91–96. https://doi.org/10.1017/S096719942200065X.
Jiang, Y., Y. He, X. Pan, P. Wang, X. Yuan, and B. Ma. 2023. Advances in oocyte maturation in vivo and in vitro in mammals. IJMS. 24:9059. https://doi.org/10.3390/ijms24109059.
Lunenfeld, B., W. Bilger, S. Longobardi, V. Alam, T. D’Hooghe, and S. K. Sunkara. 2019. The development of gonadotropins for clinical use in the treatment of infertility. Front. Endocrinol. 10:429. https://doi.org/10.3389/fendo.2019.00429.
Muhajir, M., N. W. K. Karja, M. A. Setiadi, and I. K. M. Adnyane. 2018. In vitro maturation competence of oocyte and histology observation of follicle after storage of sheep ovary at 4°C. Acta Vet Indones. 6:16–23.https://doi.org/10.29244/avi.6.2.16-23.
Negrón-Pérez, V. M., A. Al Naib, A. L. Zezeski, V. L. McCracken-Harlow, G. A. Perry, A. D. Ealy, and M. L. Rhoads. 2025. Cumulus cell expansion, nuclear maturation and embryonic development of bovine cumulus-oocyte complexes matured in varying concentrations of follicular fluid. S. Alaeejahromi, editor. PLoS ONE. 20:e0318376. https://doi.org/10.1371/journal.pone.0318376.
Pamungkas, F. A., M. A. Setiadi, and N. W. K. Karja. 2012. Characteristics and in Vitro Fertilization Ability of Ram Spermatozoa: Comparison of Epididymal and Ejaculated Spermatozoa. Med Pet. 35:38–44. https://doi.org/10.5398/medpet.2012.35.1.38.
Parera, H. 2019. Kualitas oosit kambing kacang dari status reproduksi yang berbeda. PARTNER. 24:860. http://dx.doi.org/10.35726/jp.v24i1.384.
Pei, J., X. Pan, G. Wei, and Y. Hua. 2023. Research progress of glutathione peroxidase family (GPX) in redoxidation. Front. Pharmacol. 14:1147414. https://doi.org/10.3389/fphar.2023.1147414.
Pranatasari, D., K. Kustono, and D. T. Widayati. 2016. Supplementation of gonadotropin hormone into in vitro maturation medium to increase 4 cell stadium embryo development of bligon goat. Buletin Peternak. 40:83. https://doi.org/10.21059/buletinpeternak.v40i2.9080.
Rakha, S. I., M. A. Elmetwally, H. El-Sheikh Ali, A. Balboula, A. M. Mahmoud, and S. M. Zaabel. 2022. Importance of antioxidant supplementation during in vitro maturation of mammalian oocytes. Veterinary Sciences. 9:439. https://doi.org/10.3390/vetsci9080439.
Sciorio, R., and P. Rinaudo. 2023. Culture conditions in the IVF laboratory: state of the ART and possible new directions. J Assist Reprod Genet. 40:2591–2607. https://doi.org/10.1007/s10815-023-02934-5.
Song, J., L. Xiao, Z. Zhang, Y. Wang, P. Kouis, L. J. Rasmussen, and F. Dai. 2024. Effects of reactive oxygen species and mitochondrial dysfunction on reproductive aging. Front. Cell Dev. Biol. 12:1347286. https://doi.org/10.3389/fcell.2024.1347286.
Thanaboonyawat, I., O. Makemaharn, S. Petyim, P. Laokirkkiat, and R. Choavaratana. 2016. The correlation of cumulus mucification patterns with oocyte maturation rate in vitro in FSH + LH-primed IVM cycles: a prospective study. Arch Gynecol Obstet. 293:681–686. https://doi.org/10.1007/s00404-015-3935-3.
Toosinia, S., N. Davoodian, M. Arabi, and A. Kadivar. 2024. Ameliorating effect of sodium selenite on developmental and molecular response of bovine cumulus-oocyte complexes matured in vitro under heat stress condition. Biol Trace Elem Res. 202:161–174. https://doi.org/10.1007/s12011-023-03678-0.
Tripathi, S. K., S. Nandi, P. S. P. Gupta, and S. Mondal. 2023. Antioxidants supplementation improves the quality of in vitro produced ovine embryos with amendments in key development gene expressions. Theriogenology. 201:41–52. https://doi.org/10.1016/j.theriogenology.2022.11.048.
Widayati, D. T., D. H. Fatmawati, N. Ariesta, and Kustono. 2014. The influence of follicular fluid on in vitro developmental competence media of bligon goat oocytes. J Kedokt Hewan. 8. https://doi.org/10.21157/j.ked.hewan.v8i1.1263.
Widayati, D. T., and M. Pangestu. 2020. Effect of follicle-stimulating hormone on Bligon goat oocyte maturation and embryonic development post in vitro fertilization. Vet World. 13:2443–2446. https://doi.org/10.14202/vetworld.2020.2443-2446.
Xiong, X., D. Lan, J. Li, Y. Lin, and M. Li. 2018. Selenium supplementation during in vitro maturation enhances meiosis and developmental capacity of yak oocytes. Animal Science Journal. 89:298–306. https://doi.org/10.1111/asj.12894.
Yuan, S., Y. Zhang, P.-Y. Dong, Y.-M. Chen Yan, J. Liu, B.-Q. Zhang, M.-M. Chen, S.-E. Zhang, and X.-F. Zhang. 2024. A comprehensive review on potential role of selenium, selenoproteins and selenium nanoparticles in male fertility. Heliyon. 10:e34975. https://doi.org/10.1016/j.heliyon.2024.e34975.
DOI: https://doi.org/10.21059/buletinpeternak.v50i2.115278
Article Metrics
Abstract views : 309
| 
views : 91
Refbacks
- There are currently no refbacks.
Copyright (c) 2026 Arina Dinar Nurfadilla Sastranegara, Rona Agustina, Dio Fico Felsidan Diatmono, Fransisca Gani Padmawati, Pradita Iustitia Sitaresmi, Sigit Bintara, Diah Tri Widayati
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
