Quality of Sexed Sperm of Bali Bull in Regional Artificial Insemination Center of Riau Province

https://doi.org/10.21059/buletinpeternak.v43i2.34357

Yendraliza Yendraliza(1*), Anwar Efendi Harap(2), July Handoko(3), Muhammad Rodiallah(4)

(1) Department of Animal Science, Faculty of Agriculture and Animal Science, Universitas Islam Negeri Sultan Syarif Kasim Riau, Pekanbaru, 28293, Indonesia
(2) Department of Animal Science, Faculty of Agriculture and Animal Science, Universitas Islam Negeri Sultan Syarif Kasim Riau, Pekanbaru, 28293, Indonesia
(3) Department of Animal Science, Faculty of Agriculture and Animal Science, Universitas Islam Negeri Sultan Syarif Kasim Riau, Pekanbaru, 28293, Indonesia
(4) Department of Animal Science, Faculty of Agriculture and Animal Science, Universitas Islam Negeri Sultan Syarif Kasim Riau, Pekanbaru, 28293, Indonesia
(*) Corresponding Author

Abstract


This study aimed to evaluate the quality of frozen semen of Bali bull resulted from sexing procedure on calf or offspring production with desired sex. The tested sperm of Bali bull were collected from Bali bull raised at Regional Artificial Insemination Center of Riau Province (BIBD Riau). The study was carried out in 2 stages. The first stage was X and Y chromosome separation by albumin method. The extender used in the sexing procedure is trice citrate fructose and egg yolk. The second stage was mainly testing the sexed sperm collected in 60 Bali cow in Langkat Village, Bengkalis Regency. To determine the quality of post thawing frozen semen collected from the sexing procedure, the study evaluated motility, viability, mortality, abnormality and plasma membrane integrity of the spermatozoa. The pregnancy rate, calving rate, and birth accuracy of inseminated sexed sperm to offspring’ sex were also evaluated. The evaluation resulted in motility (66.3-75.3%), viability (70-78.5%), plasma membrane integrity (60-65.8%), abnormality (6.05-8.05%), mortality (20.05-30.05%), and pregnancy rate (83.33-90%). The calving rate on this study was 100% with the birth accuracy of 81.8% for male offspring and 40% for female offspring. As conclusion, the sexed sperm evaluated on this study have fairly good fertility.

Keywords


Bali bull; Calving rate; Pregnancy rate; Sexed sperm

Full Text:

PDF


References

Afiati, F. 2004. Proporsi dan Karakteristik Spermatozoa X dan Y Hasil Separasi Kolom Albumin. Media Peternakan 27: 16-20.

Ainsworth, C., B. Nixon, R. P. S. Jansen, and R. J. Aitken. 2007. First recorded pregnancy and normal birth after ICSI using electrophoretically isolated spermatozoa. Hum. Reprod. 22: 197-200.

Aini, A. N., M. A. Setiadi, dan N. W. K. Karja. 2016.  Kemampuan fertilisasi spermatozoa sexing dan perkembangan awal embrio secara in vitro pada sapi. Jurnal Sain Veteriner 34: 225-232.

Ax, R. L., M. R. Dally, B. A. Didion, R. W. Lenz, C. C. Love, D. D. Varner, B. Hafez and M. E. Bellin. 2008. Semen evaluation. In: Reproductive in Farm Animals. 8th edn. Hafez and Hafez (eds). Lea and Febiger. Pp. 365-375.

Blottner, S., H. Bostedt, K. Mewes, and C. Pitra. 1994. Enrichment of bovine X and Y spermatozoa by free-flow electrophoresis. Zentralbl Veterinarmed A. 41: 466-474.

Diliyana, Y. F., T. Susilawati, and S. Rahayu. 2014. Keutuhan membran spermatozoa disekuensing sentrifugasi gradien densitas percoll berpengencer Andromed dan CEP-2 yang ditambahkan kuning telur. Jurnal Veteriner 15: 23-30.

Dowson, R. M. C., D. C. Elliot, W. H. Elliot, and K. M. Jones. 1986. Data for Biochemical Research. 3rd edn. Oxford Science Publication, Oxford.

Ducha, N., T. Susilawati, Aulaniam, and S. Wahyuningsih. 2012. Ultrastructure and ferlilizing ability of Limousin bull sperm after storage in CEP2 extender with and without egg yolk. Pakistan J. Biol. Sci. 15: 979-985.

Garner, D. L. and G. E. Seidel. 2008. History of commercializing sexed semen for cattle. Theriogenology. 69: 886-895.

Garner, D. L. and E. S. E. Hafez. 2000. Spermatozoa and seminal plasma. In: Reproduction in Farm Animal. Hafez, E.S.E. (ed). 7rd edn. Lea and Febiger, Philadelphia.  Pp.96-109.

Green, M. P., L. D. Spate, T. E. Parks, K. Kimura, C. N. Murphy, J. E. Williams, M. S. Kerley, J. A. Green, D. H. Keisler and R. M. Roberts. 2008. Nutritional skewing of conceptus sex in sheep: effects of a maternal diet enriched in rumen-protected polyunsaturated fatty acids (Pufa). Reprod. Bio. Endocrin. 6: 21.

Gunawan, M., E. M. Kaiin, and S. Said. 2015. Aplikasi inseminasi buatan dengan sperma sexing dalam meningkatkan produktivitas sapi di peternakan rakyat. Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia. Depok, 20 Desember 2014. Pp. 93-96.

Gunawan, M., E. M. Kaiin, and S. Said. 2017. Peningkatan produktivitas sapi Bali melalui inseminasi buatan dengan sperma sexing di Techno Park Banyumulek, Nusa Tenggara Barat. Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia. Mei 2017. pp. 216-219.

Hafez, E. S. E. and B. Hafez. 2016. X and Y chromosome-bearing spermatozoa. In Reproduction in Farm Animal. 7th edn. Hafez E.S.E. and Hafez B. (eds). Lippincott Williams and Wilkins, Philadelphia. Pp. 390-394.

Hunter, R. H. F. 1982. Reproduction of Farm Animal. School of Agriculture University of Edinburgh. Longman, London and New York.

Irikura, C. R., J. C. P. Ferreira, I. Martin, L. U. Cimenes, E. Oba, and A. M. Jorge. 2003. Follicular dynamics in buffalo heifers (Bubalus bubalis) using the GnRH-PGF2α-GnRH protocol. Buffalo J. 3: 323-327.

Jeyendran, R. S., H. H. Van der Ven and L. J. D. Zaneveld. 1992. The Hypoosmotic Swelling Test: An Update. Journal of Reproductive Systems. 29: 105-116.

Jainudeen, M. R., H. Wahid, and E. S. E. Hafez. 2000. Ovulation induction, embryo production and transfer. 2000. In: Reproduction in Farm Animal.  7th edn. Hafez E.S.E. and Hafez B. (eds). Lippincott Williams and Wilkins, Philadelphia, Pp. 405-431.

Lizuka, R., S. Kaneko, R. Aoki and T. Kobayashi. 1987. Sexing of human sperm by discontinuous Percol density gradient and its clinical application. Human Reproduction 7: 573-575.

Maxwell, W. M., G. Evans, F. K. Hollinshead, R. Bathgate, S. P. de Graaf, B. M. Eriksson, L. Gillan, K. M. Morton, and J. K. O'Brien. 2004. Integration of sperm sexing technology into the ART toolbox. Anim. Reprod. Sci. 82: 79-85.

Noakes, D. E., T. J. Parkinson, and G. C. W. England. 2017. Arthur’s Veterinary Reproduction and Obstetrics. 8th edn. Baillier Tindall, London.

Prasad, S., S. Rangasamy, and S. Satheshkumar. 2010. Sex preselectionin domestic animals. Current status and future prospects. Vet. World. 3: 346-348.

Pratiwi, W. C., D. Pamungkas, L. Affandhy, and Hartati. 2006. Evaluasi kualitas spermatozoa hasil sexing pada kemasan straw dingin yang disimpan pada suhu 5°C selama 7 hari. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner. Bogor 5-6 September 2006. Pusat Penelitian dan Pengembangan Peternakan, Bogor.

Putri, R. D. A., M. Gunawan, and E. M. Kaiin. 2015. Uji kualitas sperma sexing sapi Friesian Holstein (FH) pasca thawing. Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia. Desember 2015. 1: 2057-2061.

Ratnawati, D., L. Affandhy, W. C. Pratiwi, dan P. W. Prihandini. 2008. Pengaruh pemberian suplemen tradisional terhadap kualitas semen pejantan sapi Bali. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner, Puslitbang Peternakan 2008. Hal: 116-121.

Rasul, Z., N. Ahmad, and M. Anzar. 2001. Changes in motion characteristics, plasma membrane integrity and acrosome morphology during cryopreservation of buffalo spermatozoa. J. Androl. 22: 278-283.

Revell, S. G. and R. A. Mrode. 1994. An osmotic resistance test for bovine semen. Anim. Reprod. Sci. 36: 77-86.

Rorie, R. W. 1999. Effect of timing of artificial insemination on sex ratio. Theriogenology 52: 1273-1280.

Rosenfeld, C. S. and R. M. Roberts. 2004. Maternal diet and other factors affecting offspring sex ratio: A Review. J. Bio. Reprod. 71: 1063-1070.

Said, S. and F. Afiati. 2012. Quality of sexed sperm and it’s gender concordance on Bali cattle. Proceeding of International Conference on Livestock Production and Veterinary Technology. Oct 1, 2012 to Oct 4, 2012. Bogor, Indonesia.

Saili, T., L. O. Nafiu, L. O. Baa, S. Rahadi, A. Napirah, Syamsuddin, I. W. Sura, dan F. Lopulalan.  2017.  Efektivitas Sinkronisasi Estrus dan Fertilitas Spermatozoa Hasil Sexing pada Sapi Bali di Sulawesi Tenggara. Jurnal Veteriner 18: 353-359.

Saili, T., M. R. Toelihere, A. Boediono, dan B. Tappa. 2000. Keefektifan albumin sebagai media pemisah spermatozoa sapi pembawa kromosom X dan Y. Jurnal Hayati 7: 106-109.

Sariozkan, S. P. B., M. N. Tuncer, and Bucak. 2010. The effects of diluend egg yolk concentration used with soy bean lechitin-based - extender on semen quality of freeze bull semen. Eurasian J. Veterinary Sci. 26: 45-49.

Sitomorang, P., R. G. Sianturi, D. A. Kusumaningrum, Ross, dan Maidaswar. 2013.  Kelahiran anak sapi perah betina hasil inseminasi buatan menggunakan sexed sperma yang dipisahkan dengan kolom albumin telur. Jurnal Ilmu Ternak dan Veteriner 18: 185-191.

SNI (Standar Nasional Indonesia). 2017. Semen Beku Sapi. Badan Standardisasi Nasional (BSN). ICS. 65.020.30. Jakarta.

Steel, R. G. D. and J. H. Torrie. 1991. Principles and Procedures of Statistics. McGraw-Hill Book Co. Inc, New York.

Susilawati, T. 2003. Tingkat keberhasilan inseminasi buatan pada sapi Peranakan Ongole menggunakan semen beku hasil sexing dengan gradient konsentrasi putih telur. Jurnal Protein 20: 1410-3281.

Rosita, E. A., T. Susilawati, dan S. Wahyuningsih. 2014. Keberhasilan IB menggunakan semen beku hasil sexing dengan metode sedimentasi putih telur pada sapi PO cross. Jurnal Ilmu-Ilmu Peternakan 24: 72-76.

Sunarti, T. Saili, dan L. O. Nafiu. 2016.   Karakteristik spermatozoa sapi Bali setelah sexing menggunakan metode kolom albumin dengan lama waktu sexing yang berbeda. Jurnal Ilmu dan Teknologi Peternakan Tropis. 1: 65-76.

Vishwanath, R. and P. Shannon. 2000. Storage of bovine semen in liquid frozen state. Anim. Reprod. Sci. 62: 23-53.



DOI: https://doi.org/10.21059/buletinpeternak.v43i2.34357

Article Metrics

Abstract views : 592 | views : 534

Refbacks

  • There are currently no refbacks.




Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Buletin Peternakan (Bulletin of Animal Science) Indexed by:

 Harvard Library
Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.