The Effect of  Betel Nut Extract (Areca catethu L) on Spermatozoa Quality  (Macroscopic and Microscopic) in Male Goats

Yuni Oktaviani; Gianty Hartani Lugiana; Sadjatun Nisa; Kamelia Ningrum; Dwi Wijayanti

doi:10.21059/buletinpeternak.v46i2.47489

The Effect of Betel Nut Extract (Areca catethu L) on Spermatozoa Quality (Macroscopic and Microscopic) in Male Goats

https://doi.org/10.21059/buletinpeternak.v46i2.47489

Yuni Oktaviani⁽¹⁾, Gianty Hartani Lugiana⁽²⁾, Sadjatun Nisa⁽³⁾, Kamelia Ningrum⁽⁴⁾, Dwi Wijayanti^(5*)

(1) Department of Animal Science, Faculty of Agriculture, Perjuangan University of Tasikmalaya, Tasikmalaya, 46115, Indonesia
(2) Department of Animal Science, Faculty of Agriculture, Perjuangan University of Tasikmalaya, Tasikmalaya, 46115, Indonesia
(3) Department of Pharmacy, Faculty of Health Sciences, Perjuangan University of Tasikmalaya, Tasikmalaya, 46115, Indonesia
(4) Department of Pharmacy, Faculty of Health Sciences, Perjuangan University of Tasikmalaya, Tasikmalaya, 46115, Indonesia
(5) Department of Animal Science, Faculty of Agriculture, Perjuangan University of Tasikmalaya, Tasikmalaya, 46115, Indonesia
(*) Corresponding Author

Abstract

The purpose of this study is to determine the effect of betel nut extract on the macroscopically and microscopically quality of spermatozoa. Materials and methods were used for 16 male Ettawa goats at the age of 2 years. The preparation of betel nut extract and macroscopic observations of spermatozoa via the use of odors, colors, and volumes, as well as microscopic observations of spermatozoa through the use of mass motion, mass motility, and individual motility. The betel nut extract was administered orally for 15 days. The treatments in this study were T0 (without the provision of betel nut extract), T1 (provision of betel nut extract (90 mg/goat/day)), T2 (provision of betel nut extract (180 mg/goat/day)), T3 (provision of betel nut extract (270 mg/goat/day)). The results showed that the addition of betel nut extract was significantly different than without extracts (P<0.05). The goats without betel nut extract had the lowest quality of spermatozoa, whereas the goats treated with a betel nut extract (270 mg/goat/day) had the highest quality of spermatozoa of all the treatments. There was an increase in spermatozoa in the 10th data collection, including smell, colour, volume, mass motility, and individual motility. Furthermore, male goats were given betel nut extract at a dose of 270 mg/goat/day, which improved spermatozoa quality both macroscopically and microscopically. Subsequently, we assumed that betel nut extract could improve reproductive quality in male goats.

Keywords

Betel nut; Individual motility; Male goats; Mass motility; Spermatozoa

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References

Ax, R. L., M. R. Dally, B. A. Didion, R. W. Lenz, C.C. Love, D. D. Varner, B. Hafez, and M. E. Bellin. 2000. Artificial Insemination. In: Reproduction in Farm Animal. 7^th edn. Hafez, B and ESE Hafez (eds.) Lippincott & Wilkins, Philadelphia. Pp. 377-389.

Berger, K. E., J. Masterson, J. Mascardo, J. Grapa, I. Appanaitis, E. Temengil, B. M. Watson, and H. L. Cash. 2016. The effects of chewing betel nut with tobacco and pre- pregnancy obesity on adverse birth outcomes among palauan women. Maternal Child Health J. 20: 1696–1703.

Cindy, A., D. R. Siti, and S. Nurcholidah. 2015. Quality of Local Lamb semen in Various Quality of Ram At Different Age- Group semen Age Groups. Faculty of Animal Husbandry. Padjadjaran University.

Gangyi, X., Z. Hongping, Z. Changjun, X. Xinglin, Z. Dan, Z. Yi, and Z. Li. 2001. Research on Quality, Preservation Dilutors and Frozen Technology of Boer Goat semen. Faculty of Animal Science and Technology, Sichuan Agricultural University.

Hafez, E. S. E, and B. Hafez. 2000. X and Y Chromosome-Bearing Spermatozoa. In: Reproduction in Farm Animals 7^th edn. Hafez ESE and Hafez B. (eds). Blackwell Publishing, Australia. Pp: 390-394.

Husin, N., S. Tatik, and Kususiyah. 2007. Test of the quality of Nubian and Peranakan goats (Nubian X PE goats) and Boer goats based on storage length. Indonesian Animal Husbandry Journal 2: 57-65.

Kartasudjana, R. 2001. Artificial Insemination Techniques in Livestock. Jakarta.

Kim, S. J., M. R. Kim, S. Y. Hwang, W. J. Bae, S. Kim, S. H. Hong, J. Y. Lee, T. K. Hwang, Z. Wang, and S. W. Kim. 2013. Preliminary report on the safety of a new herbal formula and its effect on sperm quality. The World Journal of Men's Health 31: 254– 261.

Liu, T., Y. Han, T. Zhou, R. Zhang, H. Chen, S. Chen, and H. Zhao. 2019. Mechanisms of ROS-induced mitochondria-dependent apoptosis underlying liquid storage of goat spermatozoa. Aging 11: 7880–789.

Lopes, F. P. 2002. Semen Collection and Evaluation in Ram. ANS 33161. University of Florida.

Meiyanto, E., R. A. Susidarti, S. Handayani, and F. Rahmi. 2008. Ethanolic extract of areca seeds (Areca catechu L.) can inhibit proliferation and stimulate MCF-7 cell apoptosis. Indonesian Pharmacy Magazine 19: 12-19.

Partodihardjo, S. 1992. Animal Reproductive Sciences. Mutiara Sumber Widya, Jakarta.

Salehi, B., D. A. Konovalov, P. Fru, P. Kapewangolo, G. Peron, M. S. Ksenija, S. M. Cardoso, O. R. Pereira, M. Nigam, S. Nicola, G. Pignata, S. Rapposelli, S. Sestito, N. V. A. Kumar, M. de la Luz Cádiz-Gurrea, A. P. Segura-Carretero, A. Mishra, M. Sharifi-Rad, W. C. Cho, Y. Taheri, and J. Sharifi-Rad. 2020. Areca catechu-From farm to food and biomedical applications. Phytotherapy research: PTR 34: 2140–2158.

Sekosi, P. P. P., E. D. Kusumawati, and A. T. N. Krisnaningsih. 2016. Motility and viability of Etawa (PE) fresh cattle goat using cauda epididymal plasma diluent (CEP-2) at different length and save temperature. Sci. Ranch J. 4: 34-49.

Shih, L. J., J. Y. Wang, J. Y. Jheng, A. C. Siao, Y. Y. Lin, Y. W. Tsuei, Y. C. Kuo, C. P. Chuu, and Y. H. Kao. 2020. Betel nut arecoline induces different phases of growth arrest between normal and cancerous prostate cells through the reactive oxygen species pathway. Int. J. Mol. Sci. 21: 9219.

Soeprapto. 2006. Sement Shelter Method. Faculty of Animal Husbandy. Brawijaya University.

Sonjaya, H., Sutomo, and Hastuti. 2005. Effect of ionophore calcium addition on sexual quality of spermatozoa Boer Goats. J. Sci. Tech. 52: 47-50.

Toelihere, M. R. 1981. Artificial Insemination in Livestock. Space, Bandung.

Wijayanti, D., E. T. Setiatin, and E. Kurnianto. 2017. Study on postpartum estrus of guinea pigs (Cavia cobaya) using Anredera cordifolia leaf extract. Veterinary world 10: 375-379.

Wijayanti, D., E. T. Setiatin, and E. Kurnianto. 2018. Leucocyte profile and offspring production of guinea pig (Cavia cobaya) given Anredera cordifolia leaf extract. Journal of Indonesian Tropical Animal Agriculture. 43: 19-25.

Yusuf, Arifiantini, and Mulyadi. 2006. Effectiveness Glycerol Exposure Time Against Spermatozoa Motility on the semen of Sheep semen Locally Using Tris Thinners Egg yolk. Medical School Animal. Bogor Agricultural Institute.

DOI: https://doi.org/10.21059/buletinpeternak.v46i2.47489