Suplementasi Hormon Gonadotropin Pada Medium Maturasi In Vitro Untuk Meningkatkan Perkembangan Embrio Stadium 4 Sel Kambing Bligon

https://doi.org/10.21059/buletinpeternak.v40i2.9080

Dewi Pranatasari(1*), Kustono Kustono(2), Diah Tri Widayati(3)

(1) 
(2) 
(3) 
(*) Corresponding Author

Abstract


The study was carried out to investigate the effect of gonadotropin hormone supplementation into in vitro maturation medium on maturation, fertilization and embryo development of Bligon goats. This research steps consist of oocyte collection, in vitro maturation, in vitro fertilization, and in vitro embryo development. At the maturation stage the oocyte that had been collected and divided into two groups based on the maturation medium, that was tissue culture medium (TCM) with supplementation of GnRH 0 IU/mL and GnRH 25 IU/mL. Oocyte and embryo morphology data were analyzed descriptively. Maturation rate and embryo development data were analyzed by using independent sample t-test. Fertilization data was analyzed descriptively. The result showed the percentages of mature oocytes from gonadotropin supplementation of 0 IU/mL and 25 IU/mL were 54.10±25.97 and 54.89±26.44%, respectively. Expansion cumulus cells surrounding the oocytes might indicated the mature oocytes. Cleavage rate of the 2 cells stage were 13,02±11,09 and 27,01±16,65%; respectively, and for the 4 cells stage were 10,16±10,01% and 16,67±14.91%. Embryos obtained from the treatment, indicated uniform of blastomeres in the size, tight, compact, intact, and round-spherical shape. It could be concluded that supplementation of gonadotropin hormone into in vitro maturation medium could not increase the rate of oocyte maturation and 4 cell embryo development, but it could increase 2 cell embryo development of Bligon goats. Hormone supplementation could improved the maturation and embryo quality.


Keywords


Bligon goats; Embryo development; Gonadotropin hormone; In vitro fertilization

Full Text:

PDF


References

Adifa, N. S., P. Astuti, dan T. D. Widayati. 2010. Pengaruh penambahan chorionic gonadotrophin pada medium maturasi terhadap kemampuan maturasi, fertilisasi, dan perkembangan embrio secara in vitro kambing peranakan ettawa. Buletin Peternakan. 34: 8-15.

Balaban, B. and B. Urman. 2006. Effect of oocyte morphology on embryo developmet and implantation. Reprod. Biomed. Online 12: 59-66.

Basuki, P., N. Ngadiyono, dan W. Hardjosubroto. 1982. Performans produksi dan reproduksi Kambing Peranakan Etawa (PE) dan Bligon. Pusat Penelitian dan Pengembangan Ternak. Departemen Pertanian. Bogor.

Bearden, H. J. and J. W. Fuquay. 2004. Applied Animal Reproduction. Fourth Edition. Prentice Hall, New York. 77.

Boediono, A., Y. Rusianto, K. Mohamad, I. Djuwita, dan Herliatien. 2000. Perkembangan oosit kambing setelah maturasi, fertilisasi dan kultur in vitro. Media Vet. 7: 11-17.

Budisatria, I. G. S., D. T. Widayati, B. Suhartanto, Kustantinah, H. Mulyadi, dan K. A. Santosa. 2009. Bangsa-Bangsa Kambing dan Sejarah Perkembangannya di Indonesia. Subbagian Plasma Nutfah Kambing di Indonesia. Fakultas Peternakan. Universitas Gadjah Mada. Yogyakarta. p. 9.

Ciptadi, G., T. Susilawati, B. Siswanto, dan H. N. Karima. 2011. Efektivitas penambahan hormon gonadothropin pada medium maturasi msof terhadap tingkat maturasi oosit. J. Ternak Tropika. 12: 1: 108-115.

Daoed, D. M., N. Ngadiono, D. T. Widayati. 2013. Pengaruh suplementasi fetal calf serum terhadap kemampuan maturasi invitro oosit sapi. Buletin Peternakan 37: 136-142.

D’Alessandro, G. A. and Giovanni, M. 2003. Use of purified FSH and LH for embryo production cryopreservation by conventional freezing or vitrification and transfer of embryos in dairy ewes. Ital. J. Anim. Sci. 2: 131-140.

Ebner, T., M. Mozer, and G. Tews. 2006. Is oocyte morphology prognostic of embryo developmental potential after ICSI. Reproductive BioMedicine Online 12:53-58.

Gordon, I. 1994. Laboratory Production of Cattle Embrios. CAB International. UK.

Hafez, E. S. E. and B. Hafez. 2000. Micromanipulation of gametes and embryos: In Vitro Fertilization and Embryo Transfer (IVF/ET, In: Reproduction in Farm Animals, E. S. E Hafez 7th Edition. Lea and Febiger. Philadhelphia. pp. 443-465.

Hammam, A. M., C. S. Whisnant, A. Elias, S. M. Zaabel, O. Hegab, and E. M. Abu-El Naga. 2010. Effect of media, sera and hormones on in vitro maturation and fertilization of water buffallos (Bubalus bubalis). J. Anim. Vet. Adv. 9: 27-31.

Karja, N. W. K., T. Otoi, M. Murakami, M. Fahrudin, dan T. Suzuki. 2002. In vitro maturation, fertilization, and development of domestic cat oocytes recovered from ovaries collected at three stage of reproductive cycle. Theriogenology. 57: 2289-2298.

Kementrian Peternakan. 2009. Basis Data Statistik Pertanian (Hasil pencarian Berdasarkan Komoditi). Tersedia pada http://aplikasi.pertanian.go.id/bdsp/hasilKom.asp. Diakses pada 19 Agustus, 2015.

Kusindarta, D. L. 2009. Pengaruh lama maturasi dan lama inkubasi fertilisasi terhadap angka fertilitas oosit sapi peranakkan ongole secara in vitro. J. Kedokteran Hewan. 3: 185-193.

Sagirkaya, H., M. Misirlioglu, A. Kaya, N. L. First, J. J. Parrish, and E. Memili. 2007. Developmental potential of bovine oocytes cultured in different maturation and culture conditions. Anim. Reprod. Sci. 101: 225-240.

Sarwono, B. 2012. Beternak Kambing Unggul. Penebar Swadaya. Depok.

Tanaka, H. 2001. Reproductive Biology and Biotechnology. Japan International Cooperation Agency. Indonesia. 1-12.

Wattimena, J. 2011. Pematangan oosit domba secara in vitro dalam berbagai jenis serum. J. Agrinimal. 1: 22-27.

Widayati, D.T. 1999. Pengaruh Penambahan Sel-Sel Kumulus pada Media terhadap Kemampuan Maturasi Oosit, Fertilisasi, dan Perkembangan Embrio Peranakan Ongole In Vitro. Tesis Program Pascasarjana, Universitas Gadjah Mada. Yogyakarta.

Widayati, D.T., Kustono, S. Bintara, W. Asmarawati, Ismaya. 2007. Gametogenesis dan Transport Gamet. http://elisa.ugm.ac.id/community/show/ilmu-reproduksi-ternak-fapet-oleh-diah-tri-widayati/. Diakses tanggal 14 Desember 2014.

Widayati, D. T. 2008. Effect of Oocyte Morphology on Embryo Development and Implantation. Materi Kuliah Program Konsultan Fertilitas dan Endokrinologi Reproduksi (Unpublished), bagian Obsgin. Fakultas Kedokteran UGM.

Widayati, D. T., D. H. Fatmawati, N. Ariesta, dan Kustono. 2014. Penggunaan cairan folikel dalam media maturasi in vitro Oosit Kambing Bligon. J. Kedokteran Hewan 8: 64-67.

Yulnawati., M. A. Setiadi dan Boediono. 2006. Penggunaan medium CR1aa untuk produksi embrio domba in vitro. J. Ilmu Ternak dan Vet. 11: 131-136.



DOI: https://doi.org/10.21059/buletinpeternak.v40i2.9080

Article Metrics

Abstract views : 1250 | views : 1746

Refbacks

  • There are currently no refbacks.




License URL: http://journal.ugm.ac.id/buletinpeternakan/pages/view/Publication%20Ethics%20and%20Malpractice%20Statement

Buletin Peternakan (Bulletin of Animal Science) Indexed by:

 Harvard Library
Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.