NMR metabolomics revealed metabolites and bioactivity variation in Torbangun leaves Plectranthus amboinicus L. with different origins

https://doi.org/10.22146/ijbiotech.38659

Nancy Dewi Yuliana(1*), Muhammad Anwari Sugiharto(2), Hanifah Nuryani Lioe(3), Masao Goto(4), Yuko Takano Ishikawa(5)

(1) Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University, IPB Dramaga, Bogor 16680, Indonesia
(2) Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University, IPB Dramaga, Bogor 16680, Indonesia
(3) Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University, IPB Dramaga, Bogor 16680, Indonesia
(4) Functionality Evaluation Unit, Food Function Division, Food Research Institute, NARO, Tsukuba-Ibaraki, Japan
(5) Functionality Evaluation Unit, Food Function Division, Food Research Institute, NARO, Tsukuba-Ibaraki, Japan
(*) Corresponding Author

Abstract


Plectranthus amboinicus has been reported to have antidiabetic and antioxidant activities. Environmental factors might influence the plant’s secondary metabolite profile and its beneficial properties. NMR-based metabolomics was used to show phytochemical variations between specimens of P. amboinicus grown in Japan and Indonesia. The results showed that flavonoids and triterpenes were among the discriminating factors of the variation between the two groups. Targeted comparative analysis of the concentration of the specific flavonoids of the plants using a validated HPLC-MWD method showed that the Japanese samples contained a higher concentration of total flavonoids compared with the Indonesian samples. The Japanese and Indonesian samples contained 1100.6 ± 5.1 and 532.4 ± 1.8 µg/g luteolin, and 584.5 ± 7.4 and 571.7 ± 11.6 µg/g apigenin, respectively. Eriodyctiol was detected only in the Indonesian samples. Contrarily, more intensive DPPH reduction and α-glucosidase inhibition activities were found in the Indonesian samples (IC50 14.4 ± 1.2 and 24.0 ± 0.3 µg/mL for the DPPH assay, 1181.9 ± 113.5 and 4451.4 ± 290.0 µg/mL for α-glucosidase inhibition, respectively). Thus, flavonoids might not be the only group of compounds related to the aforementioned bioactivities. This should be confirmed by further research targeting other groups of compounds, such as triterpenes.


Keywords


Plectranthus amboinicus, metabolomics, flavonoids, antidiabetes and antoxidant

Full Text:

PDF


References

Andarwulan N, Yuliana ND, Hasna E, Aziz SA, Davis TD, Andarwulan N. 2014. Comparative analysis of three torbangun clones (Plectranthus amboinicus (lour.) spreng) based on phenotypic characteristics and phenolic content. Am J Plant Sci. 5(24):3673–3683. doi:10.4236/ajps.2014.524383.

Arumugam G, Swamy MK, Sinniah UR. 2016. Plectranthus amboinicus (lour.) spreng: Botanical, phytochemical, pharmacological and nutritional significance. Molecules. 21(4):369. doi:10.3390/ molecules21040369.

Brieskorn CH, Riedel W. 1977. Flavonoide aus Coleus amboinicus. Planta Med. 31(04):308–310. doi:10. 1055/s-0028-1097537.

Brieskorn CHRW. 1977. Die Triterpeiisaureii aus Coleus amboiiiicus Loureiro. Arch Pharm. 310:910–916. doi: 10.1002/ardp.19773101108.

Chang CC, Yang MH, Wen HM, Chern JC. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal. 10(3).

da Silva LAL, Faqueti LG, Reginatto FH, dos Santos ADC, Barison A, Biavatti MW. 2015. Phytochemical analysis of vernonanthura tweedieana and a validated UPLC-PDA method for the quantification of eriodictyol. Rev Bras Farmacogn. 25(4):375–381. doi: 10.1016/j.bjp.2015.07.009.

Damanik R, Wahlqvist ML, Wattanapenpaiboon N. 2006. Lactagogue effects of Torbangun, a Bataknese traditional cuisine. Asia Pac J Clin Nutr. 15(2):267–274.

El-Hawary SS, El-Sofany RH, Abdel-Monem AR, Ashour RS, Sleem AA. 2012. Polyphenolics content and biological activity of Plectranthus amboinicus (lour.) spreng growing in Egypt (Lamiaceae). Pharmacogn J. 4(32):45–54. doi:10.5530/PJ.2012.32.9.

Eriksson L, Byrne T, Johansson E, Trygg J, Vikström C. 2003. Multi- and megavariate data analysis: basic principles and applications. volume 1. Umeå: Umetrics Academy.

Govindaraju S, Arulselvi PI. 2018. Characterization of Coleus aromaticus essential oil and its major constituent carvacrol for in vitro antidiabetic and antiproliferative activities. J Herbs Spices Med Plants. 24(1):37–51. doi:10.1080/10496475.2017.1369483.

Haghi G, Hatami A. 2010. Simultaneous quantification of flavonoids and phenolic acids in plant materials by a newly developed isocratic high-performance liquid chromatography approach. J Agric Food Chem. 58(20):10812–10816. doi:10.1021/jf102175x.

Hameed A, Hafizur RM, Hussain N, Raza SA, Rehman M, Ashraf S, Ul-Haq Z, Khan F, Abbas G, Choudhary MI. 2018. Eriodictyol stimulates insulin secretion through cAMP/PKA signaling pathway in mice islets. Eur J Pharmacol. 820:245–255. doi:10.1016/j.ejphar.2017. 12.015.

Koşar M, Dorman H, Hiltunen R. 2005. Effect of an acid treatment on the phytochemical and antioxidant characteristics of extracts from selected Lamiaceae species. Food Chem. 91(3):525–533. doi:10.1016/j. foodchem.2004.06.029.

Latimer GW, editor. 2012. Official methods of analysis of AOAC International. AOAC International.

Lin LZ, Harnly JM. 2007. A screening method for the identification of glycosylated flavonoids and other phenolic compounds using a standard analytical approach for all plant materials. J Agric Food Chem. 55(4):1084–1096. doi:10.1021/jf062431s.

Lin LZ, Mukhopadhyay S, Robbins RJ, Harnly JM. 2007. Identification and quantification of flavonoids of Mexican oregano (Lippia graveolens) by LC-DADESI/MS analysis. J Food Compos Anal. 20(5):361– 369. doi:10.1016/j.jfca.2006.09.005.

Lukhoba CW, Simmonds MSJ, Paton AJ. 2006. Plectranthus: a review of ethnobotanical uses. J Ethnopharmacol. 103(1):1–24. doi:10.1016/j.jep.2005.09.011.

Mediani A, Abas F, Khatib A, Maulidiani H, Shaari K, Choi YH, Lajis N. 2012. 1H-NMR-based metabolomics approach to understanding the drying effects on the phytochemicals in Cosmos caudatus. Food Res Int. 49(2):763–770. doi:10.1016/J. FOODRES.2012.09.022.

Mphahlele RR, Stander MA, Fawole OA, Opara UL. 2014. Effect of fruit maturity and growing location on the postharvest contents of flavonoids, phenolic acids, vitamin C and antioxidant activity of pomegranate juice (cv. Wonderful). Sci Hortic. 179:36–45. doi:10.1016/ j.scienta.2014.09.007.

Murthy PS, Ramalakshmi K, Srinivas P. 2009. Fungitoxic activity of Indian borage (Plectranthus amboinicus) volatiles. Food Chemistry. 114(3):1014–1018. doi:10.1016/J.FOODCHEM.2008.10.064.

Salazar-Aranda R, Pérez-López LA, Joel LA, AlanísGarza BA, Waksman de Torres N. 2011. Antimicrobial and antioxidant activities of plants from northeast of mexico. J Evidence-based Complementary Altern Med. 2011:1–6. doi:10.1093/ecam/nep127.

Shibano M, Kitagawa S, Nakamura S, Akazawa N, Kusano G. 1997. Studies on the constituents of Broussonetia species. II. Six new pyrrolidine alkaloids, broussonetine a, b, e, f and broussonetinine a and b, as inhibitors of glycosidases from Broussonetia kazinoki Sieb. Chem Pharm Bull. 45(4):700–705. doi: 10.1248/cpb.45.700.

Tattini M, Galardi C, Pinelli P, Massai R, Remorini D, Agati G. 2004. Differential accumulation of flavonoids and hydroxycinnamates in leaves of Ligustrum vulgare under excess light and drought stress. New Phytol. 163(3):547–561. doi:10.1111/j. 1469-8137.2004.01126.x.

Viswanathaswamy AHM, Koti BC, Gore A, Thippeswamy AHM, Kulkarni RV. 2011. Antihyperglycemic and antihyperlipidemic activity of Plectranthus amboinicus on normal and alloxaninduced diabetic rats. Indian J Pharm Sci. 73(2):139– 145. doi:10.4103/0250-474x.91572.

Wang L, Weller CL. 2006. Recent advances in extraction of nutraceuticals from plants. Trends Food Sci Technol. 17(6):300–312. doi:10.1016/j.tifs.2005.12.004.

Wang M, Lamers RJAN, Korthout HAAJ, Van Nesselrooij JHJ, Witkamp RF, Van Der Heijden R, Voshol PJ, Havekes LM, Verpoorte R, Van Der Greef J. 2005. Metabolomics in the context of systems biology: bridging traditional Chinese medicine and molecular pharmacology. Phytother Res. 19(3):173–182. doi:10.1002/ptr.1624.

Yuliana ND, Khatib A, Choi YH, Verpoorte R. 2011a. Metabolomics for bioactivity assessment of natural products. Phytother Res. 25(2):157–169. doi:10.1002/ ptr.3258.

Yuliana ND, Khatib A, Verpoorte R, Choi YH. 2011b. Comprehensive extraction method integrated with NMR metabolomics: a new bioactivity screening method for plants, adenosine A1 receptor binding compounds in Orthosiphon stamineus Benth. Anal Chem. 83(17):6902–6906. doi:10.1021/ac201458n.



DOI: https://doi.org/10.22146/ijbiotech.38659

Article Metrics

Abstract views : 783 | views : 400

Refbacks

  • There are currently no refbacks.


Copyright (c) 2018 The Author(s)

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Past issues



The Indonesian Journal of Biotechnology (print ISSN 0853-8654; online ISSN 2089-2241) is published by the Research Center for Biotechnology in collaboration with the Graduate School of Universitas Gadjah Mada. The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License, and attributable to Siti Nurleily Marliana and Joaquim Baeta. Built on the Public Knowledge Project's OJS 2.4.8.1 and designed by Joaquim Baeta. Web
Analytics View website statistics.