Molecular Identification of Phenol-Degrading and Biofi lm-Forming Bacteria from Wastewater and Peat Soil
https://doi.org/10.22146/ijbiotech.9299 Arifah Khusnuryani(1*), Erni Martani(2), Tri Wibawa(3), Jaka Widada(4)
(1) Biotechnology Study Program, Universitas Gadjah Mada, Yogyakarta, Indonesia Biologi Study Program, UIN Sunan Kalijaga, Yogyakarta, Indonesia
(2) Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Faculty of Medical, Universitas Gadjah Mada, Yogyakarta, Indonesia
(4) Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author
Abstract
Phenol is hazardous aromatic pollutant which needs to be treated to reduce its hazardous effects.
Bioremediation using bacteria which can form biofi lm offer an alternative wastewater treatment that is cheaper
and environmentally safe. Eighteen strains of phenol-degrading and biofi lm-forming bacteria were isolated
from peat soil, also hospital and textile wastewater. Screening for phenol degradation ability of isolates were
performed using Folin-ciocalteau reagent, while for biofi lm formation ability were performed using microtiter
plate and crystal violet dye. Based on the ability to degrade phenol and to form biofi lm, four isolates (HP3,
DOK135, DL120, andATA6) were choosen as phenol-degrading bacteria as well as biofi lm-forming bacteria.
Based on phenotypic and genotypic characterization, isolate HP3 was highly similar to Rhodococcus equi strain
DSM20307T, while DOK135 was highly similar to Enterobacter mori strain R18-2.The results also suggested that
DL120 and ATA6 could be classifi ed to the genus of Micrococcus and Bacillus respectively
Bioremediation using bacteria which can form biofi lm offer an alternative wastewater treatment that is cheaper
and environmentally safe. Eighteen strains of phenol-degrading and biofi lm-forming bacteria were isolated
from peat soil, also hospital and textile wastewater. Screening for phenol degradation ability of isolates were
performed using Folin-ciocalteau reagent, while for biofi lm formation ability were performed using microtiter
plate and crystal violet dye. Based on the ability to degrade phenol and to form biofi lm, four isolates (HP3,
DOK135, DL120, andATA6) were choosen as phenol-degrading bacteria as well as biofi lm-forming bacteria.
Based on phenotypic and genotypic characterization, isolate HP3 was highly similar to Rhodococcus equi strain
DSM20307T, while DOK135 was highly similar to Enterobacter mori strain R18-2.The results also suggested that
DL120 and ATA6 could be classifi ed to the genus of Micrococcus and Bacillus respectively
Keywords
biofilm-forming bacteria; phenol-degrading bacteria; phenotypic and genotypic characterization
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PDF DOI: https://doi.org/10.22146/ijbiotech.9299
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