Analysis of Melamine Using Electronic Nose, Gas Chromatography-Time of Flight - Mass Spectrometry and High Performance Liquid Chromatography

https://doi.org/10.14499/jfps

Abdul Rohman.(1*), Yaakob B. Che Man(2), R. M. Hafidz(3)

(1) Faculty of Pharmacy, Gadjah Mada University
(2) Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia
(3) Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia
(*) Corresponding Author

Abstract


An  electronic  nose  (E-Nose)  has  been  used  as  rapid  analytical  technique  for  melamine detection  in  food  samples.  The  parameter  used  is melamine  retention  index  (MRI)  in  E-Nose  chromatogram.  The  value  of MRI  was  also  confirmed  using  two  dimensional  gas
chromatography-time of flight-mass spectrometry (GCxGC-TOF-MS). The value of MRI using E-Nose and GCxGC-TOF-MS was nearly close  i.e 1486 and 1540 respectively. HPLC has been used  for  quantitative  analysis  of melamine  in  some milk  products  suspected  to  contain melamine using column Nova-Pak® C18;(150 x 3.9 mm  i.d; 4 µm) at 30°C. The mobile phase
was  5 mmol  L−1  NaH2PO4  delivered  isocratically  at  flow  rate  of  1 mLmin-1 . Melamine was linear at concentration range of 0.0625 – 50.0 µg/ml with coefficient of determination (R2 ) of 0.999.

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DOI: https://doi.org/10.14499/jfps

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Journal of Food and Pharmaceutical Sciences (ISSN: 2339-0948) -  Universitas Gadjah Mada, Indonesia.