Physico-chemical Amino acid composition, fatty acid profile, functional and antioxidant properties of Spinacia oleracea L. leaf

https://doi.org/10.14499/jfps

Prabhakara Rao Pamidighantam(1*), Narsing Rao Galla(2), Narsing Rao Galla(3), Sulochanamma Guruguntla(4), Sulochanamma Guruguntla(5), Satyanarayana Akula(6), Satyanarayana Akula(7)

(1) CSIR Central Food Technological Research Institute, Resource Centre, Hyderabad, Telangana, India
(2) CSIR CFTRI Resource Centre, Hyderabad
(3) CSIR CFTRI Resource Centre, Hyderabad
(4) CSIR CTRI Resource Centre, Hyderabad
(5) CSIR CTRI Resource Centre, Hyderabad
(6) CSIR CFTRI Resource centre, Hyderabad
(7) CSIR CFTRI Resource centre, Hyderabad
(*) Corresponding Author

Abstract


Spinach (Spinacia oleracea L.) leaf powder (SLP) was prepared and its physicochemical (proximate composition, colour, mineral content, EMC-RH, protein solubility, amino acid content and fatty acid profile), functional (water absorption, fat absorption, emulsification and foam capacities) and antioxidant (DPPH, ABTS and Iron reducing power) properties were studied. SLP possessed a protein content of 31.72%, calcium (1335 mg/100g), iron (29 mg/100g) and phosphorous (278 mg/100g). Moisture sorption isotherms of SLP indicated its non-hygroscopic nature. Solubility of SLP protein in distilled water was 39.66%, which got enhanced to 72.83% in 0.1M NaCl at pH 12. The spinach leaf lipid was found to be rich in ω-3 fatty acids (67.4%). Leucine (11.87%), phenylalanine (5.9%) and valine (9.24%) were the major essential amino acids observed in SLP. Functional properties of SLP exhibited good water absorption, fat absorption and foam capacities. Antioxidant activity of SLP determined by the 2,2-azino-bis (3-ethyl benzothiazoline 6- sulphonic acid (ABTS) assay was found higher, when compared to the activities determined by diphenyl picrylhydrazyl (DPPH) and ferric reducing power assays. Protein solubility, amino acid content, fatty acid profile, functional properties and antioxidant characteristics encouraging for food and pharmaceutical applications.


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References

Aletor, A.A.O.; Ipinmoroti, K.; Chemical composition of common leafy vegetables and functional properties of their leaf protein concentrates. Food Chem. 2002, 78 (1), 63–68.

Ali, M.B.; Khandaker, L.; Oba, S.; Comparative study on functional components, antioxidant activity and color parameters of selected colored leafy vegetables as affected by photoperiods. J. Food Agric. Environ. 2009, 7(3-4), 392-398.

Amagloh, F.K.; Nyarko, E.S.; Mineral nutrient content of commonly consumed leafy vegetables in northern Ghana. African J. Food Agric. Nutri. Dev, 2012, 12(5), 6397-6408.

AOAC. Official methods of analysis of Association of official Analytical Chemists (AOAC) International (16th ed.). Association of official Analytical Chemists International. Washington, DC, USA, 1995.

Bidlingmeyer, B.A.; Cohen, S.A.; Tarvin, T.L.; Rapid analysis of amino acids using pre-column derivatization. J. Chromatogr. 1984, 336, 93–104.

Bryan, G.M.; Rolf, S.; Nadir, R.S.; Eva, S.; Moringa (Moringa oleifera) leaf meal as a source of protein in locally produced concentrates for dairy cows fed low protein diets in tropical areas. Livestock Sci. 2011, 137(1-3), 10-17.

Carlsson, R.; Waste green parts of plants as raw material for leaf protein concentrate production. Biol. Waste. 1989, 28 (2), 83–90.

Chang, N.W.; Huang, P.C.; Effects of the ratio of polyunsaturated and monounsaturated fatty acid to saturated fatty acid on rat plasma and liver lipid concentrations. Lipid., 1998, 33, 1-487.

Chang, N.W.; Chen, T.W.; Chen, F.N.; Huang, P.C.; High polyunsaturated and monounsaturated fatty acid to saturated fatty acid ratio increases plasma very low density lipoprotein lipids and reduces the hepatic hypertriglyceridemic effect of dietary cholesterol in rats. Nutr. Res. 2004, 24, 73-83.

FAO/WHO. Protein and Amino Acid Requirements in Human Nutrition. WHO, Geneva, pp. 150, 2007.

Folch, J.; Lees, M.; Stanley, G.S.; A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 1957, 226, 497-509.

Gagne, N.; Adambounou, L.T.; Physico-chemical and functional properties of roes from autumn spawning herring (Clupea herengus herengus L.). Food Res. Int. 1994, 27, 405–408.

Gokhan, Z.; Abdurrahman, A.; Gokalp-Ozmen, G.; Yavuz-Selim, C.; Julio, G.C.; Manuel, A.; Javier, V.; Nutritional quality of protein in the leaves of eleven Asphodelinespecies (Liliaceae) from Turkey. Food Chem. 2012, 135 (3), 1360–1364.

Kim, S.J.; Cho, A.R.; Han, J.; Antioxidant and antimicrobial activities of leafy green vegetable extracts and their applications to meat product preservation. Food Control 2013, 29(1), 112-120.

Lakshminarayana, G.; Jogi Pantulu, A.; Sundar Rao, K.; Lipid class and fatty acid compositions of young Amaranthus gangeticus L. leaves. J. Agric. Food Chem. 1984, 32 (6), 1361–1363.

Lamsal, B.P.; Koegel, R.G.; Gunasekaran, S.; Some physicochemical and functional properties of alfalfa soluble leaf proteins. LWT - Food Sci. Technol. 2007, 40 (9), 1520 -1526.

Memarpoor, Y.M.; Hanie, M H.; Zar, Z.H.; Antioxidant activity of protein hydrolysates and purified peptides from Zizyphus jujuba fruits. J. Function Food. 2013, 5, 62-70.

Lawhon, J.F.; Carter, C.M.; Matil, K.F.A.; Comparative study of the whipping potential of an extract from several oil seed flours. Cereal Sci. Today 1972, 17, 240-244, 246, 294.

Moore, S.; On the determination of cystine as cysteic acid. J. Biochem. 1963, 238, 235–237.

Nanjo, F.; Goto, K.; Seto, R.; Suzuki, M.; Sakai, M.; Hara, Y.; Scavenging effects of tea catechins & their derivatives on 1, 1, -diphenyl-2picrylhydrazyl radical. Free Radical Biol. Med. 1996, 21, 895-902.

Narsing Rao, G.; Govardhana Rao, D.; Chemical & functional characterization of Gum karaya (Sterculia urens L.) seed meal. Food Hydrocolloid. 2010, 24 (5), 479-485.

Narsing Rao, G.; Prabhakar Rao, P.G.; Satyanarayana, A.; Chemical, amino acid and fatty acid composition of Sterculia urens L. seed. Food Hydrocolloids, 2012, 28, 320-324.

Otles, S.; Pire, R.: Fatty acid composition of chlorella and spirulina microalgae species. J. AOAC Int. 2001, 84 (6), 1708-1714.

Pellett, L.P.; Young, V.R.; Nutritional evaluation of protein foods. UN: University Food and Nutrition Bulletin, (Suppl. 4), Tokyo, Japan, 1980.

Ranganna, S.; Hand Book of Analysis & Quality Control for Fruits & Vegetable Products, (2nd ed.). Tata McGraw-Hill Publishing Company limited, New Delhi, India, 1986.

Re, R.; Pellegrini, N.; Proteggente, A.; Yang, M.; Rice-Evans, C.; Antioxidant activity applying an improved ABTS radical cation decolourization assay. Free Radical Biol. Med. 1999, 26(9-10), 1231-1237.

Sadasivam, S.; Manickam, A.; Biochemical Methods, (2nd ed.). New Age International Publishers, New Delhi, India, pp. 59-69, 1997.

Srianta, I.; Arisasmita, J.H.; Patria, H.D.; Epriliati, I.; Ethnobotany, nutritional composition and DPPH radical scavenging of leafy vegetables of wild Paederia foetida and Erechtites hieracifolia. Int. Food Res. J. 2012, 19(1), 245-250.

Taher, F.S.; Abbassy, M.; El-Nockroshy, A.S.; Shoeb, Z.E.; Counter current extraction –soelectric precipitation of sunflower seed protein isolates. J. Sci. Food Agric. 1981, 32, 166–174.

Wang, J.C.; Kinsella, J.E.; Functional properties of novel proteins: alfalfa leaf protein. J. Food Sci. 1976, 41(1), 286-292.

Yildrim, A.; Mavi, A.; Kara, A.A.; Antioxidant & antimicrobial activities of Rumex cripsus L. extracts. J. Agric. Food Chem. 2001, 49, 4083-4089.



DOI: https://doi.org/10.14499/jfps

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Journal of Food and Pharmaceutical Sciences (ISSN: 2339-0948) -  Universitas Gadjah Mada, Indonesia.