Extraction and Fractionation of Anti-Cholesterol Compounds from Water Chestnut (Eleocharis dulcis)

https://doi.org/10.22146/jfs.106066

Hemi Adi Putra(1), Amir Husni(2*), Alim Isnansetyo(3)

(1) Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Sleman Regency, Special Region of Yogyakarta, Indonesia
(2) Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Sleman Regency, Special Region of Yogyakarta, Indonesia
(3) Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Sleman Regency, Special Region of Yogyakarta, Indonesia
(*) Corresponding Author

Abstract


Cholesterol is a significant contributor to mortality rates worldwide. The quest for effective cholesterol treatments continues, aiming to develop preventive agents with minimal adverse effects. This study seeks to elucidate the anticholesterol properties of extracts and components fractionated from water chestnut (Eleocharis dulcis). The research encompassed several phases, including sampling, extraction and fractionation, qualitative and quantitative flavonoid analysis, anticholesterol activity assays, and gas chromatography-mass spectrometry (GC-MS) analysis. The quantitative flavonoid assessment revealed that the highest flavonoid concentration was present in the ethyl acetate:methanol (5:5) fraction, totaling 79.224 mg/mL. The water chestnut extract demonstrated notable anti-cholesterol activity with an IC50 value of 13016.27 ± 5648.31 ppm, whereas the water chestnut fraction exhibited enhanced anti-cholesterol activity with an IC50 value of 42.22 ± 9.79 ppm, comparable to simvastatin. The bioactive compounds in water chestnut attributed to its anti-cholesterol effects include 7,9-Di-tert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione, n-Hexadecanoic acid, Octadecanoic acid ethyl ester, 9-Octadecenamide (Z)-, 9,12,15-Octadecatrienoic acid, 2,3-dihydroxypropyl ester, 3,4-Dimethoxycinnamic acid, and 2H-1-Benzopyran-2-one, 7-methoxy-. These findings suggest that the fraction derived from E. dulcis exhibits significant anti-cholesterol activity and holds promise for further development in the treatment of hypercholesterolemia.


Keywords


Anticholesterol; flavonoids; Eleocharis dulcis



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How to Cite this Article:

Putra, H.A., A. Husni & A. Isnansetyo. 2025. Extraction and fractionation of anti-cholesterol compounds from water chestnut (Eleocharis dulcis). Jurnal Perikanan Universitas Gadjah Mada. 27 (1): xx-xx. https://doi.org/10.22146/jfs.106066



DOI: https://doi.org/10.22146/jfs.106066

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