Antibacterial Activity of Jatropha curcas (Linn) Leaves Extract Against Vibrio harveyi and Aeromonas hydrophila

https://doi.org/10.22146/jfs.10741

Nasrullah Bai Arifin(1*), Imas Marthapratama(2), Ellana Sanoesi(3), Arief Prajitno(4)

(1) Aquaculture Study Program, Faculty of Fisheries and Marine Science, University of Brawijaya
(2) Aquaculture Study Program, Faculty of Fisheries and Marine Science, University of Brawijaya
(3) Aquaculture Study Program, Faculty of Fisheries and Marine Science, University of Brawijaya
(4) Laboratory of Parasitology and Fish Diseases Aquaculture Study Program, Faculty of Fisheries and Marine Science, University of Brawijaya
(*) Corresponding Author

Abstract


The use of natural products as an antimicrobial agent is an alternative way in the treating drug-resistant pathogens. A. hydrophila and V. harveyi are pathogenic bacteria both in freshwater and marine culture, respectively. Physic nut (Jatropha curcas, Linn) has been known as an herbal plant that has an antibacterial agent. The purpose of this study was to analyze inhibition ability of an extract of physic nut leaves on the growth of V. harveyi and A. hydrophila. In this study, water extract of physic nut leaves was determined to evaluate the antibacterial activity against V. harveyi and A. hydrophila using the Minimum Inhibitory Concentration Test (MIC) and Paper Disk Diffusion Method. The smallest concentration on the MIC was 0.10 g/ml for A. hydrophila and 0.15 g/ml for V. harveyi, respectively. The Paper Disk Diffusion Method used difference concentrations (0.10 g/ml; 0.15 g/ml; 0.20 g/ml; 0.25 g/ml; 0.30 g/ml; 0.35 g/ml; dan 0.40 g/ml) and Control (0 g/ml). The concentration of 0.35 g/ml had the highest inhibition zone diameter in 11.3±0.2 mm for A. hydrophila and 0.40 g/ml had the highest inhibition zone in 11.2±0.3 mm for V. harveyi. The result showed that water extract of physic nut leaves was able to inhibit both tested bacteria.

Keywords


Aeromonas hydrophila; paper disk diffusion; Jatropha curcas Linn; minimum inhibitory concentration test; Vibrio harveyi

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References

Adamu, L.G.O., B. Edeghagba., Omolara., M. Abiola, A.I. Elijah & O.T. Ezeokoli. 2013. Antimicrobial activity of extracts of Jatropha curcas and Calatropis procera leaves against pathogenic isolates from motorcycle helmets in Lagos metropolis. International J. of Current Microbiology and Applied Sciences. 2: 292-302.

Alamsyah, A.N. 2006. Biodiesel Jarak Pagar Bahan Bakar Alternatif Yang Ramah Lingkungan Agromedia Pustaka. Jakarta.

Arekemase, M.O., R.M.O. Kayode & A.E. Ajiboye. 2011. Antimicrobial activity and phytochemical analysis of Jatropha curcas plant against some selected microorganisms. International J. of Biology. 3: 52-59.

Austin, B. & X-H. Zhang. 2006. Vibrio harveyi: a significant pathogen of marine vertebrates and invertebrates. Letter in Applied Microbiology. 43: 119-124.

Chen, L., X. Cheng., W. Shi., Q. Lu., V. L. Go., D. Heber & L. Ma. 2005. Inhibition of growth of Streptococcus mutans, methicillin-resistant Staphylococcus aureus, and vancomycinresistant Enterococci by kurarinone, a bioactive flavonoid isolated from Sophora flavescens. J. of Clinical Microbiology. 43: 3574-3575.

Cipriano, R.C., G.L. Billock & S.W. Pyle. 1984. Aeromonas hydrophila and motile aeromonad septicemias of fish. US Fish and Wildlife publication Paper 134.

Cowan, M.M. 1999. Plant Products as Antimicrobial Agents. Clinical Microbiology Reviews 12: 564582. Cushnie, T.P.T & J.L. Andrew. 2005. Review antimicrobial activity of flavonoids. International J. of Antimicrobial Agents. 26: 343-356

Dada, E.O., F.O. Ekundayo & O.O. Makanjuola. 2014. Antibacterial activities of Jatropha curcas (Linn) on coliform isolated from surface water in Akure, Nigeria. International J. of Biochemical Science. 10: 25-30.

Daniyan, S.Y., M.E. Abalaka., O.M. Elemba & S.A. Aransiola. 2012. In vitro antimicrobial activity and phytochemical screening of Jatropha curcas seed extract. International Research J. of Pharmacy. 2: 60-64.

Edberg, S.C. 1986. Tes Kerentanan Antimikroba Dalam Antibiotika Dan Infeksi. Alih bahasa: Chandra Sanusi. CV EGC Penerbit Buku Kedokteran. Jakarta. 845 hal.

Hameed, A.S.S., K.H. Rahaman., A. Alagan & K. Yoganandhan. 2003. Antibiotic resistance in bacteria isolated from hatchery-reared larvae and post larvae of Macrobracium rosenbergii. Aquaculture. 217: 39-48.

Hazril, G.A. 2005. Evaluasi Potensi Tanin Dari Tanaman Jarak Pagar (Jatrophacurcas).http:// abstraksita.fti.itb.ac.id/?abstraksi=1&details=1& id=764&tahun=2005.

Hervio_Health, D., R.R. Colwell., A. Derrien., A. Robert-Pillot., J.M. Fournier & M. Pommepuy. Robert-Pillot., J.M. Fournier & M. Pommepuy. 2002. Occurrence of pathogenic vibrios in coastal areas of France. J. of Applied Microbiology. 92: 1123-1135

Igbinosa, O.O., E.O. Igbinosa & O.A. Aiyegoro. 2009. Antimicrobial activity and phytochemical screening of stem bark extract from Jatropha curcas (Linn). African J. of Pharmacy and Pharmacology. 3: 58-62.

Jones, G.A., T.A. Mc-Allister., A.D. Muir & K.J. Chen. 1994. Effects Of Sainfoin (Onobrychis viciifolia Scop.) Condensed Tannins on Growth and Proteolysis by Four Strains Of Ruminal Bacteria. Applied and Environmental Microbiology 60: 1374-1378.

Jun, L & N.Y.S. Woo. 2003. Pathogenicity of vibrios in fish: an overview. J. of Ocean University of Qingdao. 2: 117-128.

Kim, S.R., L. Nonaka., M.J. Oh., Lavilla-Pitago & S. Suzuki. 2003. Distribution of oxytetracycline resistance determinant tet (34) among marine bacterial isolated of a Vibrio spesies. Microbes and Environments. 18: 74-81.

Laith, A.R & M. Najiah. 2013. Aeromonas hydrophila: antimicrobial susceptibility and histopathology of isolates from diseased catfish, Clarias gariepinus (Burchell). J. Aquaculture Research Development. 5: 2-7.

Mun’im, A. 2005. Isolasi dan Elusidasi Struktur Senyawa Flavonoida dari Crotalaria Anagyroides. Majalah Ilmu Kefarmasian. 2: 2229.

Narayani, M., M. Johnson., A. Sivaraman & N. Jnanakiraman. 2012. Phytochemical and antibacterial studies on Jatropha curcas L. J. of Chemical and Pharmaceutical Research. 4: 2639-2642.

Nonaka, L., T. Isshiki & S. Suzuku. 2000. The occurrence of oxytetracycline resistance bacteria in the fish intestine and seawater environment. Microbes and Environments. 15: 223-228.

Nonaka, L., T. Isshiki & S. Suzuku. 2002. Distribution of oxytetracycline resistance determinant, tet 34 among bacteria isolated from diseased fish. Microbes and Environments. 17: 26-31.

Nyembo, K., N. Kikakedimau., H. Mutambel., N. Mbaya., T. Ekalakala & O. Bulubulu. 2012. In vitro antibacterial activity and phytochemical screening of crude extracts from Jatropha curcas Linn. Europan J. of Medical Plants. 2: 242-251.

Pelczar, M.J & E.C.S. Chan. 1988. Dasardasar Mikrobiologi jilid 2. Penerjemah: R.S Hadioetomo, Teja I, S. Sutarmi, S.L Angka. Penerbit Universitas Indonesia Press. Jakarta.

Prajitno, A. 2007a. Penyakit Ikan-Udang : Bakteri. Penerbit Universitas Negeri Malang. Malang.

Prajitno, A. 2007b. Uji Sensitivitas Bio-Aktif Alami Halimeda opuntia terhadap Bakteri Vibrio harveyi secara In Vitro (Uji Patogenitas Bakteri Vibrio harveyi). Jurnal Penelitian Perikanan. Fakultas Perikanan Universitas Brawijaya 10: 22-27.

Setha, B., A. Laga., M. Mahendradatta & Firdaus. 2014. Antibacterial activity of leaves extracts of Jatropha curcas, Linn against Enterobacter aerogenes. International journal of scientific and technology research. 3: 129-131.

Sharma, A., S. Saxena., U. Rani., S. Rajore & A. Batra. 2010. Broad-spectrum antimicrobial properties of medicinally important plant Jatropha curcas. International J. of Pharmaceutical Sciences Review and Research. 3: 11-14.

Sivaram, V. 2004. Growth and immune response of juvenile greasy groupers (Epinephelus tauvina) fed with herbal antibacterial active principle supplemented diets against Vibrio harveyi infections. Aquaculture. 237: 9-20.

Subba, B & P. Basnet. 2014. Antimicrobial activity of some medicinal plants from East and central part of Nepal. International J. of Applied Sciences and Biotechnology. 2: 88-92.

Taie, H.A.A., M.M.I. Helal., W.A. Helmy & H. Amer. 2013. Chemical composition and biological potentials of aqueous extracts of fennel (Foeniculum vulgare L). J. of Applied Sciences Research. 9: 1759-1767.

Vaseeharan, B & P. Ramasamy. 2003. Control of pathogenic Vibrio spp. By Bacillus subtilis BT23, a possible probiotic treatment for black tiger shrimp Penaeus monodon. Letters in Applied Microbiology. 3: 83-87.

Volk, W.A & F.W. Margaret.1983. Mikrobiologi Dasar. Edisi ke-5. Jilid 1. Erlangga. Jakarta.



DOI: https://doi.org/10.22146/jfs.10741

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