Pengaruh Suplementasi Probiotik A3-51 terhadap Derajat Imunitas Oreochromis niloticus Didasarkan pada Angka Kuman pada Ginjal Setelah Uji Tantang dengan Aeromonas hydrophila dan Aeromonas salmonicida achromogenes

https://doi.org/10.22146/jfs.126

Agus Irianto(1*), Hernayanti Hernayanti(2), Ning Iriyanti(3)

(1) Fakultas Biologi Universitas Jenderal Soedirman
(2) Fakultas Biologi Universitas Jenderal Soedirman
(3) Fakultas Peternakan Universitas Jenderal Soedirman
(*) Corresponding Author

Abstract


Objectives of this research was to know the effect of probiotic A3-51 supplementation on food to the total number of bacteria in kidney and mortality of Oreochromis niloticus after challenge test with Aeromonas hydrophila and Aeromonas salmonicida achromogenes. This research was consisted of two experiment, the first was to know the highest immunity level of fish based on the number of macrophage and its phagocytic activity. Meanwhile, the second was to know the immunity level of fish challenged with A. hydrophila and A. salmonicida achromogenes by intra-peritoneal injection. The experiment used Completely Randomized Design in triplicates. The result from the first experiment showed that the highest non spesific immune system response, by means number of macrophage, was found 21 days treatment. The second experiment showed that the highest total number of bacteria in kidney and mortality level were 20.23 x 108 cells/g and 46.67%, respectively, both was found in control fish injected intra-peritoneally with A. salmonicida achromogenes.

Keywords


probiotic, macrophage, total number of bacteria, mortality

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References

Bratawidjaja, K.G. 2000. Imunolodi dasar. Balai Penerbit Fakultas Kedokteran Universitas

Indonesia. Jakarta. 315 p.

Ceulemans, S., P. Coutteau, L. And J. Rotllant. 2002. Supplemented feed stimulate immune

systems of gilthead seabream. Global Aquaculture Advocate, October 2002. 5 (5): 47-49.

Chao, K-K., C-C. Chao, and W-L. Chao. 2003. Suitability of microbial indicators and their enumerating methods in the assessment of fecal pollution of subtropical freshwater enviroments. J. Microbiology and Immunology Infection. 36: 288-293.

Iriyanti, N. Dan E.A. Rimbawanto. 2001. Pengaruh suplementasi probiotik Lactobacillus sp. dalam ransum unggas terhadap aktivitas antagonisme dan kompetisi Lactobacillus sp. pada saluran pencernaan unggas. Biosfera. 18(2): 68-72.

Irianto, A. 2002. A study of probiotics effective for the control of Aeromonas salmonicida infection in fish. PhD Thesis. Edinburgh: Heriot-Watt University. 173 p.

Irianto, A. 2004. Probiotik akuakultur. Gadjahmada University Press. Yogyakarta. 125 p.

Irianto, A. 2005. Patologi ikan teleostei. Gadjahmada University Press. Yogyakarta. 256 p.

Irianto, A. and B. Austin. 2002. Use of probiotic to control furunculois in rainbow trout, Oncorhynchus mykiss (Walbaum). J. Fish Diseases. 25: 333-342.

Irianto, A. dan B. Austin. 2004. Respon imun ikan komet (Carrasius auratus) terhadap suplementasi probion A3-51 per oral melalui pakan dan kelulusan hidupnya pada uji tantang dengan Aeromonas salmonicida atipikal. Prosiding Pengendalian Penyakit pada Ikan dan Udang berbasis Imunisasi dan Biosecurity. Purwokerto: 86-90.

Kirkaúa, M., H. Uzbülek, Vavuzcan, and Yilzid. 2002. A report on spontaneous diseases in the culture of grass carp (Ctenopharyngodon idella Val. 1844). Turkey Journal of Veterinary Animal Science. 26: 407-410.

Molinari, L.M., D.O. Scoaris, R.B. pedroso, N.L.R. Bittencourt, F.C.V. Nakamura, T.U Nakamura, B.A. Abreu, and B.P.D. Filho. 2003. Bacterial microflora in the gastrointestinal tract of nile tilapia, Oreochromis niloticus, cultured in a semi-intensive system. Acta Scientiarum. 25(2): 267-271.

Roberts, R.J. 2001. Fish pathology. 3rd ed. Curchill Publisher. Livingstone. 495 p.

Soeharsono, H. 1997. Probiotik alternatif pengganti antibiotik. Buletin PPSKI. 9(X): 3-5.

Steel, R.G.D. and J.H. torrie. 1980. Principles and procedures of statistics: A biometrical approach. 2nd ed. McGraw-Hill. New York. 633 p.

Taufik, p. 2001. Ketahanan ikan baung, Mystus nemurus, terhadap patogen Aeromonas hydrophila jurnal Ilmu-ilmu Perairan.4(2):612.



DOI: https://doi.org/10.22146/jfs.126

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Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).

 

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