Antioxidant Activity and Proximate Analysis of Dry Powder from Brown Seaweed Sargassum hystrix

https://doi.org/10.22146/jfs.23885

Radipta Lailatussifa(1*), Amir Husni(2), Alim Isnansetyo(3)

(1) Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada
(2) Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada
(3) Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


This research aims to know antioxidant activity, total phenolic, nutrient, and screening phytochemistry compounds of S. hystrix dry powder. Water extract from the dried sample of brown algae (S. hystrix) was prepared and examined for its phenolic compounds. The amount of total phenolic compounds in water extract was about 11.43 g Gallic Acid Equivalent (GAE)/100 g of dry basis sample, as measured by using Folin-Ciocalteu method. The antioxidant activity (Radical Scavenging Activity/RSA) of BHT as a standard was high about 96% inhibition of radical DPPH with 1 mg dry sample/ml solvent. The IC50 of the BHT was 0.227 ± 0.001 mg of dry sample/ ml solvent. While the radical activity (RSA) of S. hystrix dry powder was about 65.28% at 1 mg dry sample/ml solvent. The IC50 of S. hystrix dry powder was 0.616 ± 0.005 mg of dry sample/ml solvent. Nutrient contents and bioactive compounds of dry powder S. hystrix were balanced to support antioxidant activity of the sample. The results suggest that this alga is a potential source of natural antioxidant.

Keywords


Antioxidant activity; Sargassum hystrix dry powder; phytochemistry; the phenolic; compound nutrient contents

Full Text:

PDF


References

Agbor, G.A., J.E. Oben., J.Y. Ngogang., C. Xinxing & J.A. Vinson. 2005. Antioxidant capacity of some herbs/spices from cameroon: a comparative study of two methods. J. Agr. Food Chem. 53: 6819-6824.

Airanthi, M.W., M. Hosokawa & K. Miyashita. 2011. Comparative antioxidant activity of edible Japanese brown seaweeds. J. Food Sci. 76: 104-111.

Ale, M.T., H. Maruyama., H. Tamauchi & J.D. Mikkelsen. 2011. Fucose-containing sulfated polysaccharides from brown seaweeds inhibit proliferation of melanoma cells and induce apoptosis by activation of caspase-3 in vitro. Mar. Drugs 9: 2605-2621.

Andayani, R., L. Yovita & Maimunah. 2008. Penentuan aktivitas antioksidan, kadar fenolat total dan likopen pada buah Tomat (Solanum lycopersicum I). J. Sains dan Teknologi Farmasi 13: 31-37.

Ankanna, S., D. Suhrulatha & N. Savithramma. 2012. Chemotaxonomical studies of some important monocotyledons. Bot. Res. Int. 5: 90-96.

AOAC. 1984. Official Methods of Analysis of The Association Analytical Chemist. Inc. Washington D. C.

AOAC. 2005. Official Method of Analysis of the Association of Official Analytical Chemist. Benyamin Franklin Station. Washington D. C.

AOAC. 2009. Integrated Total Dietary Fiber, Assay Procedure Including Resistant Starch and Non Digestible Oligosaccharides. Megazyme International Ireland.

AOAC. 2011. Integrated Total Dietary Fiber, Assay Procedure Including Resistant Starch and Non Digestible Oligosaccharides. Megazyme International Ireland.

Athukorala, Y., G.N. Ahn., Y.H. Jee., G.Y. Kim., S.H. Kim & J.H. Ha. 2009. Antiproliferative activity of sulfated polysaccharide isolated from an enzymatic digest of Ecklonia cava on the U-937 cell line. J. Appl. Phycol. 21: 307-314.

Badan Standarisasi Nasional. 2006. Cara Uji KimiaBagian 2: Penentuan Kadar Air pada Produk Perikanan, SNI-01-2354.2-2006. Jakarta.

Bahre, T.A & G.R.F. Tchouya. 2016. Comparative study of the anti-oxidant activity of the total polyphenols extracted from Hibiscus Sabdariffa L., Glycine max L., Merr., yellow tea and red wine through reaction with DPPH free radicals. Arab. J. Chem. 1-8.

Balboa, E.M., E. Conde., A. Moure., E. Falque & H. Dominguez. 2013. In vitro antioxidant properties of crude extracts and compounds from brown algae. Food Chem. 138: 1764-1785.

Boonchum, W., Y. Peerapornpisal., D. Kanjanapothi., J. Pekkoh., C. Pumas & U. Jamjai et al. 2011. Antioxidant activity of some seaweed from the gulf of Thailand. Int. J. Agr. Biol. 13: 95-99.

Budhiyanti, S.A., S. Raharjo., D.W. Marseno & I.Y.B. Lelana. 2011. Free radical scavenging, metal chelating, and singlet oxygen quenching activity of fractionated brown seaweed Sargassum hystrix extract. J. Biol. Sci. 11: 288-298.

Budhiyanti, S.A., S. Raharjo., D.W. Marseno & I.Y.B. Lelana. 2012. Antioxidant activity of brown algae Sargassum species extract from the coastline of Java Island. American J. Agr. Biol. Sci. 7: 337346.

Cahyaningrum, K., A. Husni & S.A. Budhiyanti. 2016. Aktivitas antioksidan ekstrak rumput laut cokelat (Sargassum polycystum). AGRITECH 36: 2-9.

Chandini, S.K., P. Ganesan & N. Bhaskar. 2008. In vitro antioxidant activities of three selected brown seaweeds of India. Food Chem. 107: 707-713.

Chao Li, X. Li., L. You., X. Fu & R.H. Liu. 2017. Fractination, preliminary structural characterization and bioactivities of polysaccharides from Sargassum pallidum. Carbohyd. Polym. 155: 261-270.

Chun-Yung Huang., S.J. Wu., W.N. Yang., A.W. Kuan & C.Y. Chen. 2016. Antioxidant activities of crude extracts of fucoidan extracted from Sargassum glaucescens by a compressionalpuffing-hydrothermal extraction process. Food Chem. 197: 1121-1129.

Coria-Tellez, A.V., E. Montalvo-Gonzalez., E.M. Yahia & E.N. Obledo-Vazquez. 2016. Annona muricata: a comprehensive review on its traditional medicinal uses, phytochemicals, pharmacological activities, mechanisms of action and toxicity. Arabian J. Chem. xxx: xxxxxx.

Costa, L.S., G.P. Fidelis., C.B.S. Telles., N. DantasSantos., R.B.G. Camara & S.L. Cordeiro et al. 2011. Antioxidant and antiproliferative activities of heterofucans Sargassum filipendula. Mar. Drugs. 9: 952-966.

Daneshfar, A., H.S. Ghaziaskar & N. Homayoun. 2008. Solubility of gallic acid in methanol, ethanol, water, and ethyl acetate. J. Chem. Eng. 53: 776-778.

Dawczynski, C., R. Schubert & G. Jahreis. 2007. Amino acids, fatty acids, and dietary fibre in edible seaweed products. Food Chem. 103:891899.

Dawidowicz, A.L., D. Wianowska & M. Olszowy. 2012. On practical problems in estimation of antioxidant activity of compounds by DPPH method (Problems in estimation of antioxidant activity). Food Chem. 131: 1037-1043.

Dhianawaty, D & Ruslin. 2015. Kandungan total polifenol dan aktivitas antioksidan dari ekstrak metanol akar Imperata cylindrica (L) Beauv. (Alang-alang). MKB 47: 60-64.

Dore, C.M.P., M.G.D.C.F. Alves., L.S.E.P. Will., T.G. Costa., D.A. Sabry., L.A.R.S. Rego., C.M. Accardo., H.A.O. Rocha., L.G.A. Filgueira & E.L. Leite. 2013. A sulfated polysaccharide, fucans, isolated from brown algae Sargassum vulgare with anticoagulant, antithrombotic, antioxidant and anti-inflammatory effects. Carbohyd. Polym. 91: 467-475.

Farvin, K.H.S & C. Jacobsen. 2013. Phenolic compounds and antioxidant activities of selected species of seaweedsfrom Danish Coast. J. Food Chem. 138: 1670-1681.

Harborne, J.B. 1987. Metode Fitokimia. Second Edition. Padmawinata K., Soediro I., penerjemah. Phytochemical Methods. ITB. Bandung.

Harborne, J.B. 1995. Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. ITB Publisher. Bandung.

Hifney, A.F., M.A. Fawzy., K.M. Abdel-Gawad & M. Gomaa. 2016. Industrial optimization of fucoidan extraction from Sargassum sp. and its potential antioxidant and emulsifying activities. Food Hydrocolloid. 54: 77-88.

Hong, Ye., K. Wang., C. Zhou., J. Liu & X. Zeng. 2008. Purification, antitumor and antioxidant activities in vitro of polysaccharides from the brown seaweed Sargassum pallidum. Food Chem. 111: 428-432.

Kang, C., Y.B. Jin., H. Lee., M. Cha., E. Sohn., J. Moon., C. Park., S. Chun., E. Jung., J. Hong., S.B. Kim., J. Kim & E. Kim. 2010. Brown alga Ecklonia cava attenuates type 1 diabetes by activating AMPK and Akt signaling pathways. J. Food Chem. Technol. 48: 509-516.

Khadambi, T.N. 2007. Extraction of Phenolic Compounds and Quantification of the Total Phenol and Condensed Tannin Content of Bran Fraction of Condensed Tannin and Condensed Tannin Free Sorghum Varieties. Thesis. Department of Food Science, Faculty of Natural and Agricultural Sciences, University of Pretoria.

Khalaf, N.A., A. Shakiya., A. L-Othman., E.L.-Agbar & Z.H. Farah. 2008. Antioxidant activity of some common plants. Turkish J. Biol. 32: 51-55.

Khaled, N., M. Hiba & C. Asma. 2012. Antioxidant and antifungal activities of Padina pavonica and Sargassum vulgare from the Lebanese Mediterranean Coast. Adv. Environ. Biol. 6: 4248.

Khotimah., K., Darius & B.B. Sasmito. 2013. Aktivitas senyawa aktif alga cokelat (Sargassum filipendulla) sebagai antioksidan pada minyak ikan Lemuru (Sardinella longiceps). Jurnal THPi, 1: 10-20.

Lailatussifa, R., A. Husni & A.E. Nugroho. 2016. Anti-stress activity of Sargassum polycystum extracts using a cold restraint stress model. Food Sci. Biotechnol. 25: 1-6.

Lann, K.L., C. Ferret., E. VanMee., C. Spagnol., M. Lhuillery & C. Payri. 2012. Total phenolic, size-fractionated phenolics and fucoxanthin content of tropical Sargassaceae (fucales, phaeophyceae) from the South Pacific Ocean: spatial and specific variability. Phycol. Research. 60: 37-50.

Lei, Y., C. Yang., C. Li., Q. Zhao., L. Liu., X. Fang & X.Y. Chen. 2016. Recent advances in biosynthesis of bioactive compounds in traditional Chinese medicinal plants. Sci. Bull. 61: 3-17.

Luo, H.Y., B. Wang., C.G. Yu., Y.I. Qu & G.I. Su. 2010. Evaluation of antioxidant activities of five selected brown seaweeds from China. J. Med. Plants Research. 4: 2557-2565.

Makkar, H.P.S., G. Tran., V. Heuze., S. GigerReverdin., M. Lessire., F. Lebas & P. Ankers. 2016. Seaweeds for livestock diets: A review. Animal Feed Sci. Technol. 212: 1-17.

Mishra, K., H. Ojha & N.K. Chaudhury. 2012. Estimation of antiradical properties of antioxidants using DPPH assay: A critical review and results. Food Chem. 130: 1036-1043.

Molyneux, P. 2004. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol. 26: 212

Moubayed, N.M.S., H.J. Al Houri., M.M. Al Khulaifi & D.A. Al Farraj. 2016. Antimicrobial, antioxidant properties and chemical composition of seaweeds collected from Saudi Arabia (Red Sea and Arabian Gulf). Saudi J. Biol. Sci. xxx: xxx-xxx.

Nurjanah., A., Abdulla & A. Apriand. 2011. Aktivitas antioksidan dan komponen bioaktif pada keong ipong-ipong (Fasciolaria salmo). J. Pengolahan Hasil Perikanan Indonesia, XIV: 22-29.

Pei Teng Chan., P. Matanjun., S.M. Yasir & T.S. Tan. 2015. Histopathological studies on liver, kidney, and heart of normal and dietary induced hyperlipidaemic rats fed with tropical red seaweed Gracilaria changii. J. Func. Foods 17: 202-213.

Pifarre, P., M. Simo., J.D. Gispert., P. Plaza., A. Fernandez & J. Pujol. 2015. Diazepam and Jacobson’s progressive relaxation show similar attenuating short-term effects on stress-related brain glucose consumption. Eur. Psychiat. 30: 187-192.

Plaza, M., M. Amigo-Benavent., M.D. del Castilo., E. Ibanez & M. Herrero. 2010. Facts about the formation of new antioxidants in natural samples after subcritical water extraction. Food Res. Int. 43: 2341-2348.

Pratiwi, T. 2013. Uji Aktivitas Ekstrak Metanolik Sargassum hystrix dan Eucheuma denticulatum dalam Menghambat α-Amilase dan α-Glukosidase. Universitas Gadjah Mada. Yogyakarta.

Radji, M. 2005. Peranan bioteknologi dan mikrobia endofit dalam pengembangan obat herbal. Majalah Ilmu Kefarmasian 2: 113-126.

Senevirathne, M., S.H. Kim., N. Siriwardhana., J.H. Ha., K.W. Lee & Y.J. Jeon. 2006. Antioxidant potential of Ecklonia cava on reactive oxygen species scavenging, metal chelating, reducing power and lipid peroxidation inhibition. Food Sci. Technol. Int. 12: 27-38.

Setzer, W.N. 2008. Non-intercalative triterpenoid inhibitors of topoisomerase ii: a molecular docking study. Compounds J. 1: 13-17. Sirait, M. 2007. Penuntun Fitokimia dalam Farmasi. Institut Teknologi Bandung. Bandung.

Solarin, B.B., D. A. Bolaji., O. S. Fakayode & R.O. Akinnigbagbe. 2014. Impacts of an invasive seaweed Sargassum hystrix var. fluitans (Børgesen 1914) on the fisheries and other economic implications for the Nigerian coastal waters. IOSR J. Agr. Vet. Sci. 7.

Stengel, D.B., S. Connan & Z.A. Popper. 2011. Algal chemodiversity and bioactivity: Sources of natural variability and implications for commercial application. Biotechnol. Adv. 29: 483-501.

Suresh, V., N. Senthilkumar., R. Thangam., M. Rajkumar., C. Anbazhagan., R. Rengasamy., P. Gunasekaran., S. Kannan & P. Palani. 2013. Separation, purification, and preliminary characterization of sulfated polysaccharides from Sargassum plagiophyllum and its in vitro anticancer and antioxidant activity. Process Biochem. 48: 364-373.

Teruya, T., T. Konishi., S. Uechi., H. Tamaki & M. Tako. 2007. Anti-proliferative activity of oversulfated fucoidan from commercially cultured Cladosiphon okmuranus TOKIDA in U937 cells. Int. J. Biol. Macromol. 41: 221-226.

Tuo, K., S. Beourou., A.O. Toure., K. Ouattara., S. Meite., A.A.B. Ako., S.S. Yao., D. Koffi., B. Coulibay., A. Coulibaly & A.J. Djaman. 2015. Antioxidant activities and estimation of the phenols and flavonoids content in the extracts of medicinal plants used to treat malaria in Ivory Coast. Int. J. Curr. Microbiol. App. Sci. 4: 862874.

Winarno, F.G. 2008. Kimia Pangan dan Gizi: Edisi Terbaru. Gramedia Pustaka Utama. Jakarta.

Yan, S & R. Asmah. 2010. Comparison of total phenolic contents and antioxidant activities of turmeric leaf, pandan leaf, and torch ginger flower. Int. Food Res. J. 17: 417-423.

Ye, H., K. Wang., C. Zhou., J. Liu & X. Zeng. 2008. Purification, antitumor and antioxidant activities in vitro of polysaccharides from the brown seaweed Sargassum pallidum. Food Chem. 111: 428-432.

Zafra-Stone, S., T. Yasmin., M. Bagchi., A. Chattejee., J.A. Vinson & D. Bagchi. 2007. Berry anthocyanins as novel antioxidants in human health and disease prevention. Mol. Nutr. Food Res. 51: 675-683.

Zahra, R., M. Mehrnaz., V. Farzaneh & S. Kohzad. 2007. Antioxidant activity of extract from a brown alga, Sargassum boveanum. Afr. J. Biotechnol. 6: 2740-2745.



DOI: https://doi.org/10.22146/jfs.23885

Article Metrics

Abstract views : 4860 | views : 4391

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2017 Jurnal Perikanan Universitas Gadjah Mada

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).

 

View My Stats