PENGEMBANGAN METODE LOOP-MEDIATED ISOTHERMAL AMPLIFICATION OF DNA DAN APLIKASINYA UNTUK DETEKSI KOI HERPES VIRUS PADA BEBERAPA JENIS IKAN

https://doi.org/10.22146/jfs.2948

Murwantoko Murwantoko(1*), Triyanto Triyanto(2), Dimas A. Pamungkas(3)

(1) Jurusan Perikanan Fakultas Pertanian UGM
(2) Jurusan Perikanan Fakultas Pertanian UGM
(3) Jurusan Perikanan Fakultas Pertanian UGM
(*) Corresponding Author

Abstract


The purposes of this experiment were to establish loop-mediated isothermal amplification of DNA ( LAMP) method and its application to observe the presence and duration of koi herpes virus ( KHV) in freshwater fishes after infection. The infection was carried out using 4-6 cm length of java barb (Barbodes gonionotus), grass carp (Ctenoparingodon idella), gold fish (komet) (Carassius auratus), tambaqui (Colossoma macropomum) and koi (Cyprinus carpio koi) fishes. Fishes were infected intraperitoneally with KHV inoculums and 2 fishes were sampled everyday. DNA was extracted from gill and used for diagnosis with LAMP assays. The result of this experiment showed LAMP assay can be established and gave 100 times more sensitive than conventional PCR assay. Koi was confirmed as a host of KHV. Java barb, tambaqui, gold fish and grass carp can serve as vector for KHV and the presence of KHV was detected in java barb and tambaqui for 4 days, goldfish and grass carp for 5 days after infection.


Keywords


barb, grass carp, gold fish, KHV, koi, LAMP, tambaqui

Full Text:

Full Article (PDF)



DOI: https://doi.org/10.22146/jfs.2948

Article Metrics

Abstract views : 713 | views : 772

Refbacks

  • There are currently no refbacks.




Copyright (c)



Jurnal Perikanan Universitas Gadjah Mada Indexed by:

 

 

View My Stats