Biochemical Genetic Analysis Of Three Population Of Marble Grouper, Epinephelus polypekhadion

https://doi.org/10.22146/jfs.32

Gusti Ngurah Permana(1*), Haryanti Haryanti(2), Sari Budi Moria(3), Nyoman Adiasmara Giri(4), Ketut Sugama(5)

(1) Research Institute for Mariculture Gondol
(2) Research Institute for Mariculture Gondol
(3) Research Institute for Mariculture Gondol
(4) Research Institute for Mariculture Gondol
(5) Research Centre for Aquaculture
(*) Corresponding Author

Abstract


Genetic variations of marble groupers, Epinephelus polypekhadion were evaluated to know genetic performance of fish to support fish seed production. Fifty fish samples from three locations i.e. West Nusa Tenggara, South Sulawesi and East Java were collected for analysis. Genetic analysis has been examined through allozyme electrophoresis by using 11 enzymes (ADH, GPI, SDH, IDH, LDH, ME, PGM, 6PGD, MPI, SP, EST). The result showed that 16 loci were detected, and three of them were polymorphism enzymes namely Isocitric dehydrogenase (IDH*), Glucose Phosphate Isomerase (GPI-1*) and Esterase (EST-2*). One locus (EST-2) was polymorphic in the East Java and West Nusa Tenggara populations and three loci (EST-2, GPI-1 and IDH) were polymorphic in the South Sulawesi population. The heterozygosity ranged from 0.007 to 0.034. Rogers genetic distance between population pairs were ranged from 0.005 to 0.012 (average = 0.009). Differences between genetic populations were significant (P<0.05). East Java and West Nusa Tenggara populations have same gene pool.

Keywords


allozyme electrophoretic, genetic variation, marble grouper

Full Text:

Full Article (PDF)


References

Allendorf, F.W. and F.M. Utter. 1979. Population genetic. In: Fish physiology Vol. VII. W.S. Hoar, D.J. Randall, and J.R. Bred (Eds.). Academic Press. New York. San Fransisco London: 407-454.

Ayala, F.J. 1975. Genetic differentiation during the speciation process. Evol. Biol. 8: 1-78.

Grant, W.S. 1981. Biochemical genetic variation, population structures and evolution of Atlantic and Pacific herring. Ph.D. Dissertation Univ. Washington, Seattle. USA. 43-67.

Permana, G.N., S.B. Moria, Haryanti, and K. Sugama. 2001a. Effect of domestication on genetic variability to determine the growth of milkfish, Chanos chanos using allozyme electrophoresis. Proceeding of Mariculture Technology and Sea Farming Development, March 2001. Jakarta.: 149-158.

Permana, G. N., S.B. Moria, Haryanti, and K. Sugama. 2001b. Effect of domestication on genetic variability of humpback grouper, Cromileptes altivelis using allozyme electrophoresis, Indonesian Fisheries Research Journal. 7 (1): 24-30.

Randall, J.E. 1987. A preliminary synopsis of the grouper (Perciformi: Serranidae: Epinephelinae) of Indo-Pacific region. In : Tropical Snapper and Groupers: Biology and Fisheries Management. J.J. Polovina, and S. Ralston (Eds.).,: 89-169.

Raymond, M. and F. Roussset. 1995. GENEPOP (Version 1.2); Population genetic software for exact test and ecumenicism. J. Hered. 86:248-249.

Rimmer, M.A., S. Mc Bride, and K.C. William. 2004. Advances in grouper aquaculture. Canberra. ACIAR Monograph. 110 p.

Rogers, J.S. 1972. Measures of genetic similarity and genetic distance. In: Studies in genetics. VII. M.R. Wheeler (Ed.). Univ. Texas Publ. 7213. 354 p.

Sneath, P. H. and R.R. Sokal. 1973. Numerical taxonomy. Freeman and Co. San Fransisco, California. 859 p.

Sokal, R.R. and R.G. Rohlf. 1981. Biometry Freeman and Co. San Fransisco. Callifornia. 776 p.

Sugama, K. and A. Prijono, 1998. Biochemical genetic differentiation among wild population of milkfish, Chanos chanos in Indonesia. IFRJ. IV. (I): 11-19.

Shamsudin, L. 1992. Akuakultur pinggir laut. Dewan Bahasa dan Pustaka. Kuala Lumpur. 395 p.

Taniguchi, N. and K. Sugama. 1990. Genetic variation and populations structure of red sea bream in the coastal water of Japan and in the East China Sea. Bull. JPN. Soc. Sci. Fish, 56:1069-1077.

Whitemore, D.H. 1990. Electrophoretic and isoelectric focusing techniques in fisheries management. CRC pres inc. Boca Raton, Florida. 158-169.



DOI: https://doi.org/10.22146/jfs.32

Article Metrics

Abstract views : 185 | views : 216

Refbacks

  • There are currently no refbacks.




Copyright (c)



Jurnal Perikanan Universitas Gadjah Mada Indexed by:

 

 

View My Stats