APLIKASI BAKTERIN SEBAGAI IMUNOSTIMULAN UNTUK PENCEGAHAN INFEKSI VIRAL NERVOUS NECROSIS (VNN) PADA BENIH IKAN KERAPU BEBEK, Cromileptes altivelis

https://doi.org/10.22146/jfs.8875

Des Roza(1), Fris Johnny(2*)

(1) Balai Besar Riset Perikanan Budidaya Laut Gondol
(2) Balai Besar Riset Perikanan Budidaya Laut Gondol
(*) Corresponding Author

Abstract


An experiment on application of immunostimulant bacterin in order to prevent Viral Nervous Necrosis (VNN) in seed production of humpback grouper, Cromileptes altivelis has been conducted in Gondol Research Institute for Mariculture hatchery. The experiment arranged in completely randomized design with two replicates. One thousand and five hundred fry with total length 4 cm were treated as followed ; 1 ml bacterin/L sea water by immersion (Treatment A), 1 ml bacterin/kg pellet by oraly (Treatment B), and without bacterin as a control (C). Bacterin was immersion and oraly every 10 days for 60 days rearing period. Challenge test with VNN was carry out at day 30 and 60. At the end of experiment the non-spesific immune were examined. Result of challenge test performed at day 30 showed that treated fish gave positive response to bacterin treatment. The highest survival showed by treatment B (65.0%) followed by treatment A(50.0%) and C (22.5%). The same result also showed after challenge test at day 60. The highest survival was 72.5% (treatment B) followed by 67.5% (treatment A) and 35.0% (control). Phagocytic activity (PA) of treatment B was 15.5%, and 14.0% from treatment A compared to 8.0% in control. Lysozyme activity (LA) of treated groups were also higher than control namely 1.8 cm for treatment B followed by 1.6 cm for treatment A, and only 1.2 cm in control. Addition of bacterin immunostimulant in feed gives is survival rate higher compared to way of immersion and control. Statistically non-spesific immune response of humpback grouper was significant different among treatment (P<0,05).


Keywords


Humpback grouper, immunostimulant, prevention, VNN

Full Text:

PDF


References

Anderson, D.P. 1996. Environmental factors in fish health: immunological aspects. In : The fish immune system: organism, pathogen and environment. G. Iwama and T. Nakanishi (Eds.),. Academic Press. San Diego, California. p. 289-310

Arimoto, M., K. Mori, T. Nakai, K. Muroga and I. Furusawa. 1993. Pathogenicity of the causative agent of viral nervous necrosis disease in striped jack, Pseudocaranx dentex (Bloch and Schneider). Journal of Fish Disease. 16:461 – 469.

Dalmo, R.A., B. Martinsen, T.E. Horsberg, A. Ramstad, C. Syvertsen, R. Seljelid and K. Ingebrigtsen. 1998. Prophylactic effect of β(1,3)-D-glucan (laminaran) against experimental Aeromonas salmonicida and Vibrio salmonicida infection. Journal of Fish Disease, 21:459-462.

Ellis, A.E. 1993. Lysozyme assays. In : Techniques in fish immunology-1. Stolen, J.S., T.C. Fletcher, D.P. Anderson, B.S. Roberson, W.B. van Mulswinkel. (Eds.). SOS Publications, Fair Haven, NJ 07704-3303. USA. p.101-103

Itami, T., M. Kando, M. Uozu, A. Suganuma, T. Abe, A. Nakagawa, N. Suzuki and Y. Takahashi. 1996. Enhancement of resistance against Enterococcus seriolicida infection in yellowtail, Seriola quinqueradiata (Temminck & Schlegel), by oral administration of peptidoglycan derived from Bifidobacterium thermophylum. Journal of Fish Disease. 19:185-187.

Johnny, F., I. Koesharyani, D. Roza, Tridjoko, N.A. Giri dan K. Suwirya. 2001. Respon ikan kerapu bebek, Cromileptes altivelis terhadap imunostimulan peptidoglycan melalui pakan pelet. Jurnal Penelitian Perikanan Indonesia. Vol. VII. (4):52-56.

Johnny, F., Zafran, D. Roza dan I. N. A. Giri. 2002. Pengaruh vitamin C dalam pakan terhadap perubahan hemositologi ikan kerapu bebek, Cromileptes altivelis. Jurnal Aquaculture Indonesia. 3(1):27-34.

Johnny, F., D. Roza, K. Mahardika, Zafran dan A. Prijono. 2005. Penggunaan imunostimulan untuk meningkatkan kekebalan non-spesifik benih ikan kerapu lumpur, Epinephelus coioides terhadap infeksi virus irido. Jurnal Penelitian Perikanan Indonesia. Vol. 11(5):75 - 84.

Johnny, F. dan D. Roza. 2004a. Penggunaan imunostimulan pada larva ikan kerapu lumpur, Epinephelus coioides untuk meningkatkan kekebalan terhadap infeks virus irido. Prosiding Seminar Nasional Penyakit Ikan dan Udang IV, di Purwokerto, 18-19 Mei 2004. Hal. 80-85.

Johnny, F. dan D. Roza. 2004b. Pengaruh penyuntikan imunostimulan peptidoglikan terhadap peningkatan tanggap kebal non-spesifik ikan kerapu macan, Epinephelus fuscoguttatus. Jurnal Aquaculture Indonesia. Volume 5(2): 109-115.

Kawakami, H., N. Shinohara and M. Sakai. 1998. The non-specific immunostimulation and adjuvant effects of Vibrio anguillarum bacterin, M-glucan, chitin and Freud’s complete adjuvant against Pasteurella piscicida infection in yellowtail. Journal of Fish Pathology, 33(4):287-292.

Klontz, G.W. 1997. Fish Hematology. In : Techniques in fish immunology-3 (2nd ed). Stolen, J.S., T.C. Fletcher, A.F. Rowley, J.T. Zelikoff, S.L. Kaattari, S.A. Smith. (Eds.). SOS Publications, Fair Haven, NJ 07704-3303. USA. p.121-131

Midtlyng, P.J., Reitan, L.J. and Speilberg, L. 1996. Experimental studies on the efficacy and side-effects of intraperitoneal vaccination of Atlantic salmon (Salmo salar L.) against furunculosis. Journal of Fish & Shellfish Immunology 6;335-350.

Mori, K., K. Mushiake and M. Arimoto. 1998. Control measures for viral nervous necrosis in striped jack. Journal of Fish Pathology. 33(4), 443-444.

Mushiake, K., T. Nishizawa, T. Nakai, I. Furusawa and K. Muroga. 1994. Control of VNN in striped jack ; Selection of spawners based on the detection of SJNNV gene by Polymerase chain reaction (PCR). Journal of Fish Pathology 29(3):93-98.

Nakai, T., H.D. Nguyen, T. Nishizawa, K. Muroga, M. Arimoto and Otsuki. 1994. Outbreaks of viral nervous necrosis in Epinephelus moara and tiger puffer Takifugu rubripes. Journal of Fish Pathology. (29):211-212.

Nishizawa, T., K. Mori, T. Nakai, I. Furusawa and K. Muroga. 1994. Polymerase chain reaction (PCR) amplication of RNA of striped jack nervous necrosis virus (SJNNV). Journal of Dis. Aquat. Org. 18. 103 – 107.

Rowley, A.F. 1993. Collection, separation and identification of fish leucocytes. In : Techniques in Fish Immunology-1. Stolen, J.S., T.C. Fletcher, D.P. Anderson, B.S. Roberson, W.B. van Mulswinkel. (Eds.).. SOS Publications, Fair Haven, NJ 07704-3303. USA. p.114-136.

Roza, D., F. Johnny dan Tridjoko. 2004. Peningkatan imunitas yuwana ikan kerapu bebek, Cromileptes altivelis terhadap infeksi viral nervous necrosis (VNN) dengan cara vaksinasi melalui perendaman. Jurnal Penelitian Perikanan Indonesia Vol. 10 No.1 Tahun 2004. Hal. 61-70.

Rukyani, A., E. Silvia, A. Sunarto dan Taukhid. 1997. Peningkatan respon kebal non-spesifik pada ikan lele dumbo (Clarias sp) dengan pemberian imunostimulan (-glucan). Jurnal Penelitian Perikanan Indonesia II(1):1-10.

Santarem, M., B. Novoa and A. Figueras. 1997. Effect of -Glucan on the non-specific immune responses of turbot (Schopthalamus maximus L.). Journal of Fish & Shellfish Immunology. 7:429-437.

Secombes, C.J. 1996. The nonspecific immune system; cellular defences. In : The fish immune system: organism, pathogen and environment. G. Iwama and T. Nakanishi (Eds.). Academic Press. San Diego, California. p. 63-95.

Siwicki, A.K. and D.P. Anderson. 1993. Immunostimulation in fish; measures the effects of stimulants by serological and immunological methods, International Workshop and Training Course in Poland. 15 p.

Thompson, K.D., J.H. Lilley, S.C. Chen, A. Adams and R.H. Richards. 1999. The immune response of rainbow trout (Oncorhynchus mykiss) against Aphanomyces invadans. Journal of Fish&Shellfish Immunology. 9;195-210.

Vadstein, O. 1997. The use of immunostimulatin in marine larviculture: possibilities and challenges. Journal of Aquaculture. 155:401-417.

Yuasa, K., I. Koesharyani, D. Roza, F. Johnny and Zafran. 2001. Manual for PCR procedure ; Rapid diagnosis on Viral Nervous Necrosis (VNN) in grouper. Lolitkanta-JICA Booklet No. 13.35 pp.



DOI: https://doi.org/10.22146/jfs.8875

Article Metrics

Abstract views : 875 | views : 979

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 Journal of Fisheries Sciences



Jurnal Perikanan Universitas Gadjah Mada Indexed by:

 

 

View My Stats