* Corresponding Author
Uji Fitokimia dan Aktivitas Antibakteri Tumbuhan Pranajiwa (Euchresta horsfieldii (Lesch.) Benn.)
https://doi.org/10.22146/jik.40157 Amalia Indah Prihantini(1*), Krisnawati Krisnawati(2), Anita Apriliani Dwi Rahayu(3), Yosephin Martha Maria Anita Nugraheni(4), Gipi Samawandana(5)
(1) Balai Penelitian dan Pengembangan Teknologi Hasil Hutan Bukan Kayu - Badan Litbang dan Inovasi, Kementerian Lingkungan Hidup dan Kehutanan, Jl.Dharma Bhakti No. 7, Desa Langko, Kec. Lingsar, Lombok Barat, 83371- NTB
(2) Balai Penelitian dan Pengembangan Teknologi Hasil Hutan Bukan Kayu - Badan Litbang dan Inovasi, Kementerian Lingkungan Hidup dan Kehutanan, Jl.Dharma Bhakti No. 7, Desa Langko, Kec. Lingsar, Lombok Barat, 83371- NTB
(3) Balai Penelitian dan Pengembangan Teknologi Hasil Hutan Bukan Kayu - Badan Litbang dan Inovasi, Kementerian Lingkungan Hidup dan Kehutanan, Jl.Dharma Bhakti No. 7, Desa Langko, Kec. Lingsar, Lombok Barat, 83371- NTB
(4) Balai Penelitian dan Pengembangan Teknologi Perbenihan Tanaman Hutan - Badan Litbang dan Inovasi, Kementerian Lingkungan Hidup dan Kehutanan Jl.Pakuan Ciheuleut PO BOX 105, Bogor - Jawa Barat
(5) Balai Penelitian dan Pengembangan Teknologi Hasil Hutan Bukan Kayu - Badan Litbang dan Inovasi, Kementerian Lingkungan Hidup dan Kehutanan, Jl.Dharma Bhakti No. 7, Desa Langko, Kec. Lingsar, Lombok Barat, 83371- NTB
(*) Corresponding Author
Abstract
Euchresta horsfieldii merupakan tanaman obat yang dikenal di Nusa Tenggara Barat dan Bali sebagai pranajiwa. Pada penelitian ini telah dilakukan analisis fitokimia dan aktivitas antibakteri dari akar, batang, daun, dan biji pranajiwa. Pengujian aktivitas antibakteri dilakukan terhadap bakteri Bacilus subtilis Inacc-B334, Staphylococccus aureus Inacc-B4, dan Escherchia coli Inacc-B5. Hasil analisis fitokimia menunjukkan bahwa alkaloid sebagai komponen senyawa yang paling dominan pada pranajiwa dan terdeteksi di setiap bagian tanaman. Bagian akar pranajiwa terdeteksi memiliki komponen senyawa yang paling bervariasi seperti alkaloid, tanin, flavonoid, saponin, dan terpenoid. Analisis GC-MS dari batang, akar, dan biji pranajiwa menunjukkan mome inositol, sophoridane, dan asam lemak seperti asam palmitat dan asam stearat sebagai komponen utamanya. Adapun uji aktivitas antibakteri pranajiwa menunjukkan bagian batang dan akar memiliki aktivitas antibakteri terhadap S. aureus Inacc-B4 dan E. coli Inacc B-5, sedangkan bagian biji memiliki aktivitas antibakteri terhadap B. subtilis Inacc-B-334 dan S. aureus Inacc-B4. Hasil-hasil penelitian tersebut dapat mendukung penelitian terkait potensi E. horsfieldii sebagai sumber alternatif obat antibakteri.
Phytochemical Test and Antibacterial Activity of Pranawija (Euchresta horsfieldii (Lesch.) Benn.)
AbstractEuchresta horsfieldii is a medicinal plant known in West Nusa Tenggara and Bali as pranajiwa. This study investigated phytochemical analysis and antibacterial activity of roots, stems, leaves, and seeds of E. horsfieldii. The samples were analyzed for their antibacterial activity against Bacilus subtilis Inacc-B334, Staphylococccus aureus Inacc-B4, and Escherchia coli Inacc-B5. The phytochemistry result indicated that alkaloids was the most dominant constituent of E. horsfieldii as it was detected in all parts of the plant. GC-MS analysis of the stems, roots, and seeds showed mome inositol, sophoridane, and fatty acids such as palmitic acid and strearic acid as the main components. The roots had the most varied constituents with detection of alkaloids, tannins, flavonoids, saponins, and terpenoids. Further, antibacterial activity assay showed that the stems and roots had antibacterial activity against S. aureus Inacc-B4 and E. coli Inacc B-5, whereas the seeds had antibacterial activity against B. subtilis Inacc-B-334 and S. aureus InaccB4. The result of the present study supports the investigation on potentiality of E. horsfieldii as alternative source for antibacterial agents.
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Agoramoorthy G, Chandrasekaran M, Venkatesalu, Hsu MJ. 2007. Antibacterial and antifungal activities of fatty acid methyl esters of the blindyour-eye mangrove from India. Brazilian Journal of Microbiology 38:739-742.
Ahmad I, Beg AZ. 2001. Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. Journal of Ethnopharmacology 74(2): 113-123.
Chen X, Mukwaya E, Wong MS, Zhang Y. 2014. A systematic review on biological activities of prenylated flavonoids. Pharmaceutical Biology 52(5):655-60.
Choi JS, ParkNH, Hwang SY, SohnJK, KwakI, Cho KK, Choi IS. 2012. The antibacterial activity of various saturated and unsaturated fatty acids against several oral pathogens. Journal of Environmental Biology 34:673-676.
Darma IDP, Tirta IG, Ardaka IM. 20u. Study autekologi pranajiwa (Euchresta horsfieldii (Lesch.) Benn) di Bukit Pengelengan, Desa Pancasari, Kecamatan Sukasada, Kabupaten Buleleng, Bali. Buletin Kebun Raya 14(1). e24601519. doi: http://dx.doi.org/10.14203/bkr.v14i1.55.
El-Bassuony A, AbouZid S. 2010. A new prenylated flavonoid with antibacterial activity from propolis collected in Egypt. Natural Production Community 5(1):43-45.
Farrington M, Brenwald N, Haines D, Walpole E. 1992. Resistance to desiccation and skin fatty acids in outbreak strains of methicillin-resistant Staphylococcus aureus. Journal of Medical Microbiology 36:56-60.
Ferlinahayati, Syah YM, Juliawati LD, Hakim EH. 2013. Flavanones from the wood of Morus nigra with cytotoxic activity. Indonesian Journal of Chemistry 13(3):205-208.
Gunawan IWG, Ratnayani O, Putra IPGS. 2016. Isolasi senyawa golongan triterpenoid dan uji toksisitas ekstrak etanol batang pranajiwa (Euchresta horsfieldii (Lesch) Benn) terhadap larva udang (Artemia salina Leach). Jurnal Kimia 10(2):212-218.
Harborne JB. 1987. Metode fitokimia penuntun cara modern menganalisis tumbuhan. Terjemahan Kosasih Padmawinata dan Iwang Soediro. Hlm. 1-8. Penerbit ITB, Bandung.
Hernani. 2011. Pengembangan biofarmaka sebagai obat herbal untuk kesehatan. Buletin Teknologi Pascapanen Pertanian 7(1):20-29.
Kumar NR, Reddy JS, Gopikrishna G, Solomon KA. 2012. GC-MS determination of bioactive constituents of Cycas beddomei cones. International Journal of Pharma and Bio Sciences 3(3):344-350.
Lemmens RHMJ, Bunyapraphatsara N. 2003. Plant resources of South-East Asia no. 12(3): medicinal and poisonous plants 3. Backhuys Publisher, Leiden. PROSEA Foundation, Bogor.
Liliwirianis N, Zain WZWM, Kassim J, Karim SA. 2011.
Antimicrobial activity of plant extracts against Bacillus subtilis, Staphylococcus aureus, and Escherichia coli. E-Journal of Chemistry 8(1): 282-284.
Masoko P, Mokgotho MP, Mbazima VG, Mampuru LJ. 2008. Biological activities of Typha capensis (Typhaceae) from Limpopo province South Africa. African Journal of Biotechnology7:3743-3748.
McGaw LJ, Jager AK, Van Staden J. 2002. Isolation of antibacterial fatty acids from Schotia brachypetala. Fitoterapia 73(5):431-433.
Mizuno M, Tanaka T, Matsuura N, linuma M, Cheih C. 1990a. Two flavanones from Euchresta horsfieldii. Phytochemistry 29(8):2738-2740.
Mizuno M, Tanaka T, Tamura K, Matsuura N, linuma M, Phengklai C. 1990b. Flavonoids in the roots of Euchresta horsfieldii in Thailand. Phytochemistry 29(8):2663-2665.
Mizuno M, Matsuura N, linuma M, Tanaka T, Phengklai C. 1990c. Isoflavones from stems of Euchresta horsfieldii. Phytochemistry 29(8): 2675-2677
Mogea JP, Gandawidjaya D, Wriadinata H, Nasution RE, Irawati. 2001. Tumbuhan langka Indonesia. Pusat Penelitian dan Pengembangan Biologi – LIPI, Bogor.
Nascimento GGF, Lacoatelli J, Freitas PC, Silva GL. 2000. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian Journal of Microbiologi 31:247-256.
Putranti RS. 2013. Skrining fitokimia dan aktivitas antioksidan ekstrak rumput laut Sargassum duplicatum dan Turbinaria ornata dari Jepara. Tesis (tidak dipublikasikan). Fakultas Perikanan dan Ilmu Kelautan Universitas Negeri Diponegoro, Semarang
Sari KAI, Gunawan IWG, Putra KGD. 2015. Kapasitas antioksidan senyawa golongan triterpenoid pada daun pranajiwa (Euchresta horsfieldii lesch benn). Jurnal Kimia 9(1):61-66.
Seanago CT & Ndip RN. 2012. Identification and antibacterial evaluation of bioactive compounds from Garcinia kola (Heckel) seeds. Molecules 17:6569-6584.
Seidel V, Taylor PW. 2004. In vitro activity of extracts and constituents of Pelagonium against rapidly growing mycobacteria. International Journal of Antimicrobial Agents 23:613-619.
Seo HS, Kim KH, Kim DY, Park BK, Shin NS, Kim JH, Youn H. 2013. GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum. Journal of Veterinary Science 14(3):241-248.
Sohn HY, Son KH, Kwon CS, Kang SS. Antimicrobial and cytotoxic activity of 18 prenylated flavonoids isolated from medicinal plants: Morus alba L., Morus mongolica Schneider, Brouunetia papyrifera (L.) Vent, Sophora flavescens Aitand Echinosophora koreensis Nakai. Phytomedicine (7-8):666-672.
Sumiati E. 2014. Uji aktivitas antibakteri ekstrak kloroform dan ekstrak etanol biji bidara laut (Strychnos ligustrina Bl) terhadap Staphylococcus aureus ATCC 25923 dan Salmonella thypi. Biogenesis 2(1):1-10.
Sutomo, Mukaromah L. 2010. Autekologi purnajiwa (Euchresta horsfieldii (Lesch) Benn (Fabaceae) di sebagian Kawasan Hutan Bukit Tapak Cagar Alam Batukahu Bali. Jurnal Biologi 14(1):24-28.
Tirta IG, Ardaka IM, Darma IDP. 2010. Studi fenologi dan senyawa kimia pronojiwo (Euchresta horsfieldii (Lesch.) Benn.). Buletin Penelitian Tanaman Rempah dan Obat 21(1):28-36.
Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. 2011. Phytochemical screening and extraction: a review. Internationale Pharmaceutical Sciencia 1(1): 98-106
Wadood A, Ghufran M, Jamal SB, Naeem M, Khan A, Ghaffar R, Asnad. 2013. Phytochemical analysis of medicinal plants occuring in local area of Mardan. Biochemistry & Analytical Biochemistry 2(4):e216u009. doi: 10.4172/2161-1009.1000144.
DOI: https://doi.org/10.22146/jik.40157
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