Isolasi dan Karakterisasi Mikroba Indigenus Penghasil Biosurfaktan dari Eco-enzyme

Nastiti Trikurniadewi; Anindi Lupita Nasyanka; Farida Nur’aini; Rikha Anggun Novitasari; Arha Addina Illahi

doi:10.22146/jkesvo.101533

Isolasi dan Karakterisasi Mikroba Indigenus Penghasil Biosurfaktan dari Eco-enzyme

https://doi.org/10.22146/jkesvo.101533

Nastiti Trikurniadewi^(1*), Anindi Lupita Nasyanka⁽²⁾, Farida Nur’aini⁽³⁾, Rikha Anggun Novitasari⁽⁴⁾, Arha Addina Illahi⁽⁵⁾

(1) Program Studi Sarjana Terapan Teknologi Laboratorium Medis, Fakultas Kesehatan Universitas Muhammadiyah Gresik
(2) Program Studi D3 Farmasi, FakultasKesehatan Universitas Muhammadiyah Gresik
(3) Program Studi Sarjana Terapan Teknologi Laboratorium Medis, Fakultas Kesehatan Universitas Muhammadiyah Gresik
(4) Program Studi Sarjana Terapan Teknologi Laboratorium Medis, Fakultas Kesehatan Universitas Muhammadiyah Gresik
(5) Program Studi Sarjana Terapan Teknologi Laboratorium Medis, Fakultas Kesehatan Universitas Muhammadiyah Gresik
(*) Corresponding Author

Abstract

Latar Belakang: Biosurfaktan berpotensi sebagai alternatif pengganti surfaktan sebagai bahan tambahan formulasi obat. Keamanan produk biosurfaktan masih menjadi permasalahan dalam aplikasi biosurfaktan di bidang farmasi. Oleh karena itu, perlu dilakukan penelitian terkait biosurfaktan dari mikroba yang tidak bersifat patogen.

Tujuan: Penelitian ini bertujuan untuk mendapatkan mikroba indigenus eco-enzyme yang tidak bersifat patogen dan memiliki kemampuan untuk menghasilkan biosurfaktan.

Metode: Penelitian bersifat observasional dan eksperimental dengan mengisolasi mikroba yang ada pada sampel eco-enzyme kemudian menguji kemampuannya dalam menghasilkan biosurfaktan. Isolasi bakteri dilakukan dengan menggunakan media De Man Rogosa and Sharpe Agar (MRSA) dengan kondisi mikroaerofilik. Karakterisasi mikroba meliputi makroskopis, mikroskopis dan biokimia. Deteksi kemampuan isolat dalam menghasilkan biosurfaktan dievaluasi melalui uji hemolisis, uji drop collapse, aktivitas emulsifikasi dan tegangan permukaan.

Hasil: Penelitian ini mendapatkan 4 isolat potensial penghasil biosurfaktan yaitu E1, E2, E3, dan E4. Karakteristik keempat isolat berbeda secara makroskopis, mikroskopis dan biokimianya, isolat tersebut merupakan kelompok bakteri dan yeast. Keempat isolat menghasilkan biosurfaktan, aktifitas emulsifikasi tertinggi pada supernatan E3 sebesar 50,4%, tegangan permukaan terendah pada supernatan E1 sebesar 52,2 Nm/m, serta keempat supernatan isolat menunjukkan positif drop collapse.

Kesimpulan: Mikroba indigenus eco enzyme berpotensi menghasilkan biosurfaktan

Keywords

Bakteri;Biosurfaktan;Eco enzyme;Probiotik;Yeast

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DOI: https://doi.org/10.22146/jkesvo.101533