Keragaman Genetika Xanthomonas axonopodis pv. glycines Asal Kedelai Varietas Edamame di Indonesia

https://doi.org/10.22146/jpti.11814

Andi Khaeruni(1*), Antonius Suwanto(2), Budi Tjahjono(3), Meity S. Sinaga(4)

(1) Fakultas Pertanian Universitas Haluoleo
(2) Departement Biologi Fakultas MIPA Institut Pertanian Bogor
(3) Departemen Proteksi Tanaman Fakultas Pertanian Institut Pertanian Bogor
(4) Departemen Proteksi Tanaman Fakultas Pertanian Institut Pertanian Bogor
(*) Corresponding Author

Abstract


Xanthomonas axonopodis pv. glycines cause bacterial pustule disease caused a serious disease in Edamame cultivation in Indonesia. We collected a total of 29 X. axonopodis pv. glycines isolates from Edamame fields at Jember, Ciawi, Cipanas and Bogor. The genetic diversity analysis of all isolates employing ARDRA and ISR technique showed six and seven different DNA profile, respectively. Therefore there are at least seven strains of X. axonopodis pv. glycines infected Edamame in Indonesia. Both CPI from Cipanas and JA4 from Sukorejo Jember isolates possess unique DNA profle and genetically are not closely related to other isolates.

Keywords


Xanthomonas axonopodis pv. glycines; Edamame; ARDRA and ISR techniques

Full Text:

PDF


References

Borneman J, Skroch PW, O'Sullivan KM, Palus JA, Rumjamek NG, Jansen JL, Nienhuis J, Triplett EW. 1996. Molecular microbial diversity of an agricultural soil in Wisconsin. Appl Environ Microbial 62:1935-1943.

Boyer SL, Flechtner VR, Johansen JR. 2001. Is the 16S-23S rRNA internal transcribed spacer region a good tool for use in molecular systematics and population genetics? A case study in Cyanobacteria. Mo!. Biol. Evol. 18:1057-1069.

Giyanto, 1998. Deteksi Xanthomonas axonopodis pv. glycines dengan teknik PCR-RFLP gen 16S- rRNA. [tesis]. Program Pascasarjana, Institut Pertanian Bogor.

Hwang PL, Harsono K.D, Shaw PD. 1992. Use of detached soybean cotyledons for testing pathogenicity of Xanthomonas campestris pv. glycines. Plant Dis. 76: 182-183.

Khaeruni A. 2005. Keragaman genetik dan pengembangan metode deteksi cepat penyebab pustul bakteri (Xanthomonas axonopodis pv. glycines) pada kedelai. [Disertasi]. Program Pascasarjana, Institut Pertanian Bogor.

Lazo GR, Gabriel DW. 1987. Conservation of plasmid DNA sequences and pathovar identification of strains of Xanthomonas campestris. Phytopahtology 44:448-453.

Marchesi MT, Sato T, Weightman AJ, Martin TA, Fry JC, Hiom SJ, Wade GR, 1998. Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16Sr RN A. Appl. Environ. Microbiol. 64:795-799

Mesak FM, Suwanto A, Tjahjono B, Guhardja E. 1994. Modifikasi bioesei kotiledon kedelai untuk uji patogenitas Xanthomonas campestris pv. glycines, J. Il. Pert. Indon. 4:77-82.

Riupassa PA, Suwanto A. 2004. Keragaman genetik bakteri metilotrof fakultatif berpigmen merah muda pada beberapa sayuran lalaban. Hayati J. Biosains. 11:153-158

Rukayadi, Y 1995. Analisis profil DNA genom sejumlah isolat Xanthomonas campestris pv. glycines dengan menggunakan elektroforesis gen medan berpulsa (Pulsed-Field Gel Electrophoresis) [Tesis]. Progam Pascasarjana Institut Pertanian Bogor.

Sambrook J, Russel DW. 2001. Molecular cloning: A Laboratory manual. Ed ke-3. Cold Spring Harbor Lab. Press. New York.

Schaad NW. 1988. Laboratory guide for identification of plant pathogenic bacteria, Ed ke-2. APS Press.

St Paul, Minnesota Woese CR, Kandke 0, Wheelis ML. 1990. Toward a natural system of organism: Proposal for the domain Archaea, Bacteria, and Eukarya. Proc. Natl. Acad. Sci. 87:4576-4579.

Yuhana, M. 1999. Analisis profil DNA Genom sejumlah isolat vibrio berpendar asal perairan laut dan tambak udang di Indonesia (Tesis ).Program Pascasarjana, Institut Pertanian Bogor.

Yusuf M, Hala Y, Suwanto A. 2002. Keragaman genetika bakteri tanah dari rizosfer kapas transgenik dan non transgenik di Soppeng, Sulawesi Selatan. J. Mikrobiol. Indonesia 7:39-43.



DOI: https://doi.org/10.22146/jpti.11814

Article Metrics

Abstract views : 3546 | views : 1135

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2007 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics