* Corresponding Author
Antifungal Potency of Secondary Metabolites Produced by Endophytic Bacteria against Pathogenic Fungi Pyricularia oryzae Cav.
https://doi.org/10.22146/jpti.48392 Fitri Widiantini(1*), Mia Rahmah Qadryani(2), Fuji Hartati(3), Endah Yulia(4)
(1) Department of Plant Pests and Diseases, Faculty of Agriculture, University of Padjadjaran Jln. Raya Bandung-Sumedang Km. 21 Jatinangor, Sumedang, West Java 45363
(2) Study Program of Agriculture, Faculty of Agriculture, University of Padjadjaran Jln. Raya Bandung-Sumedang KM 21 Jatingor, Sumedang, West Java 45363
(3) Study Program of Agriculture, Faculty of Agriculture, University of Padjadjaran Jln. Raya Bandung-Sumedang KM 21 Jatingor, Sumedang, West Java 45363
(4) Department of Plant Pests and Diseases, Faculty of Agricuture, University of Padjadjaran Jln. Raya Bandung-Sumedang Km. 21 Jatinangor, Sumedang, West Java 45363
(*) Corresponding Author
Abstract
Blast disease caused by Pyricularia oryzae Cav. is one of the most important diseases on rice. One of the alternative controlling methods in P. oryzae is biological control through the utilization of secondary metabolites produced by endophytic bacteria. The study aimed to determine the antifungal potency of secondary metabolites produced by rice endophytic bacteria against P. oryzae. The experiment was conducted using 9 endophytic bacteria isolated rice (Os1, Os2, Os3, Os4, Os5, Os6, Os7, Os8, and Os10). Each isolates were grown in ISP2 liquid media and the secondary metabolites compounds were extracted using two different solvents; methanol and ethyl acetate : methanol (4:1) (v/v). The effect of secondary metabolites was tested using agar well diffusion method. The results demonstrated that the secondary metabolites extracted by both solvents have antifungal effect on the growth of P. oryzae. The highest growth inhibition was shown by secondary metabolites extracted by ethyl acetate : methanol (4:1) from Os1 (42%) and Os3 (39%). Antifungal activity of the secondary metabolites was indicated by the formation of clear zone. HPLC (High Performance Liquid Chromatography) analysis showed the differences of peaks and retention time between secondary metabolites produced by Os1 and Os3 which has antifungal activity and secondary metabolites produced by Os10 that did not show the antifungal activity.
Keywords
Full Text:
PDFReferences
Akpotu, M., P. Eze, C. Abba, B. Umeokoli, C. Nwachukwu, F. Okoye, & C. Esimone. 2017. Antimicrobial Activities of Secondary Metabolites of Endophytic Fungi Isolated from Catharanthus roseus. Journal of Health Sciences 7: 15-22.
Ainun, K. 2018. Aktivitas Bakteri Endofit Asal Tanaman Padi dalam Mendegradasi Kitin, Melarutkan Fosfat, dan Menghasilkan Asam Sianida (HCN). Skripsi. Fakultas Pertanian Universitas Padjadjaran, Jatinangor. 51 hlm.
Barry, A., M.B. Coyle, C. Thornsberry, E.H. Gerlach, & R.W. Harkinson. 1979. Methods of Measuring Zone of Inhibition with the Bauer-Krisby Disk Susceptibility Test. Journal of Clinical Microbiology 10: 885-900.
Cieśla Ł, & R. Moaddel. 2016. Comparison of Analytical Techniques for the Identification of Bioactive Compounds from Natural Products. Natural Product Reports 33: 1131-1145.
Dewi, T.K., D. Agustiani, & S. Antonius. 2017. Secondary Metabolites Producion by Actinomycetes and their Antifungal Activity. International Conference on Biological Science 6: 256-264.
Ikeda, S., T. Okubo, M. Anda, H. Nakashita, M. Yasuda, S. Sato, T. Kaneko, S. Tabata, S. Eda, A. Momiyama, K. Terasawa, H. Mitsui, & K. Minamisawa. 2010. Community and Genome Based Views of Plant Associated Bacteria: Plant-bacterial Interactions in Soybean and Rice. Plant Cell Physiology 51: 1398–1410.
Ortiz-Castro, R., H.A. Conteras, L. Macias, & J. Lopez-Bucio. 2009. The Role of Microbial Signals in Plant Growth and Development. Plant Signaling & Behavior 4: 701-712.
Prayudi, B. 2009. Pengendalian Penyakit Blas pada Tanaman Padi (Kasus di Lahan Sawah Irigasi Sri Agung, Tanjung Jabung Barat, Jambi). p. 508-516. Prosiding Lokakarya Percepatan Penerapan IPTEK, BBP2TP, 11-12 Desember 2007.
Sajid, I., K.A. Shaaban, & S. Hasnain. 2011. Identification, Isolation and Optimization of Antifungal Metabolites from the Streptomyces malachitofuscus ctf9. Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology] 42: 592-604.
Schumann, G.L., & G.J. D’ Arcy. 2012. Hungry Planet, Stories of Plant. The American Phytopathological Society. St Paul, Minnesota, USA. 294 p.
Sehajpal, A., S. Arora, & P. Kaur. 2009. Evaluation of Plant Extract against Rhizoctonia solani Causing Sheath Blight of Rice. The Journal of Plant Protection Sciences 1: 25-30.
Srivastava, D., Md. Shamim, M. Kumar, A. Mishra, P. Pandey, D.Kumar, P. Yadav, M.H. Siddiqui, & K.N. Singh. 2017. Current Status of Conventional and Molecular Interventions for Blast Resistance in Rice. Rice Science 24: 299-321.
Suleimana, M.M., L.J. Mcgaw, V. Naidoo, & J.N. Eloff. 2009. Detection of Antimicrobial Compounds by Bioautography of Different Extracts of Leaves of Selected South African Tree Species. African Journal of Traditional, Complementary, and Alternative Medicines 7: 64-78.
Widiantini, F., A. Herdiansyah, & E. Yulia. 2017. Biocontrol Potential of Endophytic Bacteria Isolated from Healthy Rice Plant against Rice Blast Disease (Pyricularia oryzae Cav.). KnE Life Sciences 26: 287-95.
DOI: https://doi.org/10.22146/jpti.48392
Article Metrics
Abstract views : 2265
| 
views : 1272
Refbacks
- There are currently no refbacks.
Copyright (c) 2019 Jurnal Perlindungan Tanaman Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View website statistics