Prevalensi Strain Avian Pathogenik Escherichia coli (APEC) Penyebab Kolibasilosis pada Burung Puyuh

wahyu Prihtiyantoro sarwohadi

doi:10.22146/jsv.23358

Prevalensi Strain Avian Pathogenik Escherichia coli (APEC) Penyebab Kolibasilosis pada Burung Puyuh

https://doi.org/10.22146/jsv.23358

wahyu Prihtiyantoro sarwohadi^(1*)

(1) Akademi Peternakan Brahmaputra Yogyakarta
(*) Corresponding Author

Abstract

PREVALENCE OF AVIAN PATHOGENIC Escherichia coli (APEC) STRAINS ON COLIBACILLOSIS IN QUAIL

Wahyu Prihtiyantoro¹⁾, Khusnan¹⁾, Mitra Slipranata²⁾^† dan Imron Rosyidi²⁾

¹Akademi Peternakan Brahmaputra, Jl. Ki Ageng Pemanahan, Nitikan,

Sorosutan Umbulharjo Yogyakarta,Tlp. 0274-384370, E-mail: wahyumatra@gmail.com

²Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Jl. Fauna 2, Karangmalang,

Yogyakarta 55281

ABSTRACT

Avian Pathogenic Escherichia coli (APEC) is a pathogen that causes colibacillosis in poultry, including salpingitis, omphalitis, cellulitis, swollen head syndrome, coligranuloma yolk sac inflammation, and air sacs inflammation. APEC is a zoonotic strain which spread through raw meat and processed meat products of animals and birds. In this research, the isolation and the identification of Escherichia coli were done by using selective media MacConkey, Kligger Iron Agar, and Gram staining. To analyse genetopically, polymerase chain reaction (PCR) was used to detect 16SrRNA genes, vt1 genes, and vt2 genes. Thirty one (55,36%) isolates of 56 specimens collected from quail were detected as Escherichia coli. The detection of APEC strains towards 31 Escherichia coli isolates were done by using polymerase chain reaction (PCR) with vt1 and vt2 specific primer. From the results showed that 32,26% (10/31) was APEC strains and 67.74% was non-APEC strains. From 10 isolates, 90% had vt1 gene and 10% had vt2 gene. Escherichia coli isolates were found in eyes (32,26%), infraorbital sinus fluid (32,26%), nasal fluid (16,20%), also in lungs, air sacs, ascites, and heart for 3,2% each. The isolates could not be found in the specimens from the skull. These isolates are zoonotic and have an impact on human health.

Keywords: APEC, colibacillosis, quail, vt1 and vt2

Keywords

APEC, collibacillosis, quail, vt1 and vt2

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References

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DOI: https://doi.org/10.22146/jsv.23358