Perbandingan Dua Desinfektan dalam Mengeliminasi Virus Avian Influenza H5N1 pada Telur Tetas

Umar Suryanaga; Retno D. Soejoedono; Ni Luh Putu Ika Mayasari

doi:10.22146/jsv.27622

Perbandingan Dua Desinfektan dalam Mengeliminasi Virus Avian Influenza H5N1 pada Telur Tetas

https://doi.org/10.22146/jsv.27622

Umar Suryanaga^(1*), Retno D. Soejoedono⁽²⁾, Ni Luh Putu Ika Mayasari⁽³⁾

(1) Pascasarjana Program Studi Mikrobiologi Medik Sekolah Pascasarjana, Institut Pertanian Bogor
(2) Balai Karantina Pertanian Banjarmasin
(3) Departemen Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner Fakultas Kedokteran Hewan, Institut Pertanian Bogor
(*) Corresponding Author

Abstract

Avian Influenza (AI) is a zoonotic viral disease in birds which demands priority on control and measures. Spread of AI virus can occur directly or indirectly. The use of disinfectant and handling of hatching egg waste into one of the actions that must be applied in hatchery to control the spread of AI virus. This research aim to compared two types of desinfectant in eliminating AI virus. The research was designed into 6 groups. Group I was SAN (Specific Antibody Negative) eggs as untreated negative control, group II was SAN egg treated by fumigation using potassium permanganate (KMnO4) and formalin in room temperature for 20 minutes, group III was SAN eggs soaked in benzalkolnium chloride (BKC) in room temperature for 30 seconds, group IV was SAN contaminated by AI H5N1 virus and fumigated by potassium permanganate and formalin in room temperature for 20 minutes, group V was SAN eggs contaminated by AI H5N1 virus and then soaked in benzalkonium chloride in room temperature for 30 second, and group VI was SAN eggs contaminated by AI H5N1 virus in room temperature for 10 minutes as positive control. AI H5N1 virus detection was done by using RT-PCR (Reverse Transcription Polymerase Chain Reaction) and confirmed by isolation in Embyronated Chicken Egg. The result of this research showed that the use of potassium permanganate and formalin disinfectant gave little better performance compared to benzalkoniun chloride in eliminating AI H5N1 virus on hatching eggs.

Keywords

Formalin; KMnO4; Benzalkonium Chloride; Contamination; RT-PCR

Full Text:

PDF

References

BARANTAN. Badan Karantina Pertanian. (2006). Keputusan Badan Karantina Pertanian Nomor 316.a/Kpts/PD.670.320/L/11/06 tentang Petunjuk Teknis Tindakan Karantina Hewan terhadap Media Pembawa HPAI. Badan Karantina Pertanian. Jakarta.

BKP BJM. Balai Karantina Pertanian Kelas I Banjarmasin. (2015). Laporan Tahunan 2015. Balai Karantina Pertanian Kelas I Banjarmasin. Banjarmasin.

Beato, M.S., Capua, I., Alexander, D.J. (2009). Avian influenza viruses in poultry products: a review. Avian. Pathol. 38 (3):193-200.

Cadirci, S. (2009). Disinfection of hatching eggs by formaldehyde fumigation – a review. Arch. Geflügelk. 73 (2): 116-123.

Cappucci Jr, D.T., Johnson, D.C., Brugh, M., Smith, T.M., Jackson, C.F., Pearson, J.E., Senne, A.D. (1985). Isolation of Avian Influenza virus (subtype H5N2) from chicken eggs during a natural outbreak. Avian. Disease. 29 (4):1195-2000.

Furuta, K., Maruyama, S. (1981). Bacterial contamination on eggs during incubation and hatching, and of fluffs of newly-hatched chicks. Brit. Poult. Sci. 22 (3): 247-254.

Kamps, B.S., Hoffmann, C., Preiser, W. (2006). Influenza Report 2006. Flying Publisher. South Africa.

OIE. World Organization for Animal Health. (2008). Avian Influenza. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Chapter 6.3.8. World Organization for Animal Health. France.

OIE. World Organization for Animal Health. (2009). Manual of Standards for Diagnostic Test and Vaccines for Terrestrial Animals. Chapter 2.3.4. World Organization for Animal Health. France.

OIE. World Organization for Animal Health. (2015). Terrestrial Manual Avian Influenza (Infection with Avian Influenza Viruses). Chapter 2.3.4. World Organization for Animal Health. France.

Perkins, L.E.L., Swayne, D.E. (2003). Comparative Susceptibility of Selected Avian and Mammalian Species to a Hong Kong–Origin H5N1 High-Pathogenicity Avian Influenza Virus. J. American. Associ. Avian. Patol. 958-965.

Promkuntod, N., Antarasena, C., Prommuang, P., Prommang, P. (2006). Isolation of avian influenza virus a subtype H5N1 from internal contents (albumen and allantoic fluid) of Japanese Quail (Coturnix coturnix japonica) eggs and oviduct during a natural outbreak. Annals. New York. Academy. Scienc. 1081:171-173.

Sudaryani, T., Santoso. (1999). Pembibitan Ayam Ras. Penebar Swadaya. Jakarta.

Supranto, J. (2000). Teknik Sampling untuk Survei dan Eksperimen. PT Rineka Cipta. Jakarta.

Turblin, V. (2008). Disinfection of hatching eggs importance and practical aspects. Hatchery expertise online. Ceva animal health Asia Pasific. ceva.asiapasific@ceva.com. Diunduh Juli 12, 2017.

USAID. United States Agency International Development. (2013). Indonesia’s Poultry Value Chain. http://pdf.usaid.gov/pdf_docs/pbaaa047.pdf. Diunduh Juli 12, 2017.

WHO. World Health Organization. (2005). Recommended laboratory test to identify avian influenza A virus in specimens from humans. World Health Organization. Geneva.

WHO. World Health Organization. (2007). Recommendations and laboratory procedures for detection of avian influenza A(H5N1) virus in specimens from suspected human cases. World Health Organization. Geneva.

Yamamoto, Y., Nakamura, K., Yamada, M., Mase, M. (2010). Persistance of avian influenza virus (H5N1) in feather detached from bodies infected domestic ducks. Appl. Environ. Microbiol. 76: 5496-5499.

DOI: https://doi.org/10.22146/jsv.27622