Respons Imun Ayam Petelur Pascavaksinasi Newcastle Disease dan Egg Drop Syndrome

Gusti Ayu Kencana(1*), I Nyoman Suartha(2), Daniel Raja Bonar Nainggolan(3), Agatha Seren Lumban Tobing(4)

(1) Fakultas Kedokteran Hewan, Universitas Udayana
(2) Fakultas Kedokteran Hewan, Universitas Udayana
(3) Fakultas Kedokteran Hewan, Universitas Udayana
(4) Fakultas Kedokteran Hewan, Universitas Udayana
(*) Corresponding Author


Some viral diseases in poultry could lead to huge losses to the farmers. Newcastle Disease (ND) and Egg Drop Syndrome (EDS) are a group of infectious viral disease that can cause the decreasein egg production. Newcastle Disease is caused by Avian paramyxovirus type 1 (PMV-1) Paramyxoviridae family. The causative agent of EDS is Duck adenovirus-I Adenoviridae family. Both of these diseases affect the economic losses to the poultry. The main action to prevent hens from ND and EDS virus diseases is vaccination. The success of
vaccination can be tested by serology. ND and EDS virus characteristically agglutinate hen’s erythrocyte they have Hemagglutine protein on virus envelope so can be tested by hemagglutination. The study was conducted on
a commercial poultry farm in order to determine the success of vaccination against ND and EDS. The hens were vaccinated by Newcastle Disease-Infectious Bronchitis- Egg Drop Syndrome (ND-IB-EDS) inactivated vaccines.
Serological test was conducted in pre and post vaccination by using microtiter hemagglutination test. The antibody titre is expressed in units of HI log2. The results of the study, the mean antibody titer against ND pre vaccination
was 4,53 ± 1,356 HI log2 and antibody titre in 2nd, 3rd and 4th week were 8,67 ± 0,617 HI log2, 7,73 ± 1,335 HI log2 and 5,20 ± 0,862 HI log2 post vaccination. Antibody titre against EDS pre vaccination was 0 ± 0,000 HI log2 and antibody titre post vaccination in 2nd, 3rd and 4th week were 7 ± 1,363 HI log2, 7,27 ± 1,438 HI log2 and 7,6 ± 1,056 HI log2. It showed that ND-IB-EDS inactivated vaccines is serological protective for ND and EDS titres.


Newcastle Disease; Egg Drop Syndrome; ND-IB-EDS inactivated vaccines; serology

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(ACFAF) ASEAN Cooperation in Food, Agriculture

and Foresty. 2012. ASEAN Standards for

Animal Vaccines, Second Edition. Livestock

Publication Series. http:/


categ ory/publication-3. (2 April 2016).

Aiyer-Harini P., Ashok-Kumar H.G., Kumar

GP.,Shivakumar N. 2013. An Overview

ofImmunologic Adjuvants-A Review. J

Vaccines Vaccine 4(1): 1-4.

Aldous EW, Mynn JK, Banks J, Alexander DJ (2003).

A molecular epidemiological study of avian

paramyxovirus type 1 (Newcastle disease

virus) isolates by phylogenetic analysis of

a partial nucleotide sequence of the fusion

protein gene. J. Avian Pathol. 32, 239-256.

Alexander D.J. and Senne D.A. 2008b. Newcastle

Disease and Other Avian Paramyxoviruses.

In: A Laboratory Manual for the Isolation,

Identification and Characterization of Avian

Pathogens, Dufour-Zavala L. (Editor in

Chief) Swayne D.E., Glisson J.R., JackwoodM.W., Pearson J.E., Reed W.M, Woolcock P.R., 4th ed., American Association of Avian

Pathologists, Athens, GA, 135–141.

Al-Habeeb M.A., Mohamed M.H.A., Sharawi

S. 2013. Detection and characterization of

Newcastle disease virus in clinical samples

using real time RT-PCR and melting curve

analysis based on matrix and fusion genes

amplification. Veterinary World 6(5):239-243.

Ashraf, A and Shah, M. S. 2014. Newcastle

Disease: Present status and future

challenges for developing countries.

African Journal of Microbiology Research

(5): 411-416.

Badar, S.T., Siddique, M., Ali, R., dan Rasool,

M.H. 2006. Serological Status of Egg Drop

Syndrome in Breeders and Commercial Layers

in Manshera District. Pakistan vet. J. 26(1):33-35.

Begum, J.A., Chowdhury, E.H., Parvin, R., Matin,

M.A., Giasuddin, M., Bari, A.S.M., Islam, M.R.

Detection of Egg Drop Syndrome Virus

by Polymerase Chain Reaction. International

Journal of Livestock Research3(2): 112-116.

Bidin, Z., Lojkic, I., Mikec, M., Pokric, B. 2007.

Naturally Occurring Egg Drop Syndrome

Infection in Turkeys. ACTA VET BRNO 76:415-421.

Cattoli, G., Susta, L., Terregino, C., Brown, C.

Newcastle disease: a review of field

recognition and current methods of laboratory

detection. JVDI 23(4): 637-656

Cardoso, W.M., Aguiar, F.J.L.C., Romão, J.M.,

Oliveira, W.F., Salles, R.P.R., Teixeira, R.S.C.,

Sobral, M.H.R. 2005. Effect of Associated

Vaccines on the Interference between

Newcastle Disease Virus and Infectious

Bronchitis Virus in Broilers. Brazilian J of

Poultry Sci 7(3): 181-184.

(CFSPH) The Center for Food Security and Public

Health. 2006. Egg Drop Syndrome. www. Oktober 2015).

Cho S.H., Kwon H.J., Kim T.E., Kim J.H., Yoo H.S.,

Park M.H., Park Y.H. & Kim S.J. (2008).

Characterization of a recombinant Newcastle

disease virus vaccine strain. Clin. Vaccine

Immunol., 15 (10), 1572–1579.

Ditjennak. 2014. Manual Penyakit Unggas.

Direktorat Kesehatan Hewan Direktorat

Jenderal Peternakan dan Kesehatan Hewan

Kementerian Pertanian. Hal 36.

Hafez M. 2011. Avian Adenoviruses Infections with

Special Attention to Inclusion Body Hepatitis/

Hydropericardium Syndrome and Egg Drop

Syndrome. Pak Vet J 31(2): 85-92.

Hewajuli, D.A dan Dharmayanti, N.L.P.I. 2015.

Peran Sistem Kekebalan Non-spesifik dan

Spesifik pada Unggas terhadap Newcastle

Disease. Wartazoa Vol. 25(3): 135-146.

Indriani R, Dharmayanti INLP. 2013. Studi Efikasi

Vaksin Bivalen AI Isolat Lokal terhadap

Beberapa Karakter Genetik Virus AI subtipe

H5N1. Jurnal Biologi Indonesia 9(1): 21-30.

Kencana, GA.Y., Suartha, IN., Simbolon, MP.,

Handayani, AN., Ong, S., Syamsidar.,

Kusumastuti, A. 2015. Respon Antibodi

Terhadap Penyakit Tetelo pada Ayam yang

Divaksin Tetelo dan Tetelo-Flu Burung.Jurnal

Veteriner Vol 16 (2): 283-290.

Kencana, GAY., Suartha, N., Paramita, NMAS.,

Handayani, AN. 2016. Vaksin Kombinasi

Newcastle Disease dengan Avian Influenza

Memicu Imunitas Protektif Pada Ayam Petelur

Terhadap Penyakit Tetelo dan Flu Burung.

Jurnal Veteriner Vol 17(2): 257-264.

Mayahi, M., Talazadeh, F., Aslahi, H. 2013. Effect

of the Commercial Mixed Live Newcastle

Disease and Infectious Bronchitis Vaccines

and the Use of Two Separate Vaccines

Given Simultaneously on Systemic

Antibody Responses. Iranian Journal of

Virology. 7(3): 17-21

Moomivand, H., Bassami, M.R., Faramarzi, S.,

Stabraghil, E., Ghaedi, A., Ghabel, H.,

Zarghami, A., Banaei, M. 2013. Serological

and Clinical Survey of Newcastle Disease in Broiler Chickens of East Azarbayjan by HITests. Euro J Exp Bio 3(6):311-314.

OIE (2012). Newcastle disease. Manual of

Diagnostic Tests and Vaccines for Terrestrial

Animals. Chapter 2.3.14. http://www.oie.

int/ international-standard-setting/terrestrialmanual/


Orsi MA., Doretto Jr, L, Camillo SCA., Reischak

D., Ribeiro S.A.M., Ramazzoti A., Mendonça

A.O., Spilki FR., Buzinaro M.G., Ferreira

HL., Arns CW. 2010. Prevalence of Newcastle

Disease virus in Broiler chickens (Gallus

gallus) in Brazil. Brazilian J. Microbiol.41:349-357.

Salihu, A.E., Joannis, T.M., Onwuliri, F.C., Ibu,

J.O., Masdooq, A.A., Muazu, A., Haruna, G.,

Ngbede, J. 2010. Serological Evidence of

Egg Drop Syndrome’1976 (EDS’76) in Freerange

Chickens at Chicken Market Sites in Jos,

Nigeria. Turk. J. Vet. Anim. Sci 34(4): 403-406.

Saliu, O.J., Sanda, M.E., Audu, S.I. 2009. Adoption

of Vaccination Against Newcastle Disease by

Rural Poultry Women Farmers in the North

Central Zone of Nigeria. Int. J. Poult. Sci 8

(5): 500-503.

Suresh, P., Shoba, K., Rajeswar, J.J. 2013. Incidence

of egg drop syndrome – 1976 in Namakkal

district, Tamil Nadu, India. Vet. World


Wibowo, M.H., Amanu, S. 2010. Perbandingan

Beberapa Program Vaksinasi Penyakit

Newcastle Disease pada Ayam Buras.J. Sain

Vet. Fakultas Kedokteran Hewan. Universitas

Gadjah Mada. Yogyakarta. 28 (1): 1-9.

World Organization for Animal Health. 2009.

Newcastle Disease: Aetiology, Diagnosis,

Prevention, and Control References, OIE

Technical Disease Cards. OIE Scientific and

Technical Department, Thailand.


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