* Corresponding Author
PERBANDINGAN TITER ANTIBODI AYAM BROILER YANG DIVAKSIN PADA UMUR 7 DAN 14 HARI MENGGUNAKAN VAKSIN AVIAN INFLUENZA HETEROLOG SUBTIPE H5N2
https://doi.org/10.22146/jsv.327 Ukon Susetyo(1*), Michael Haryadi Wibowo(2)
(1)
(2)
(*) Corresponding Author
Abstract
The aim of this study was to compare the titer antibody respons induced by different vaccination program
using heterologous avian influenza H5N2 subtype at day seven and 14 in broiler chicken. A number of 120
broiler chickens was divided into three groups, each consisted of 40 birds and kept since day old chicken in
standard maintenance facilities. Group one as the treatment group was vaccinated at 7 days of age using
heterologous vaccine H5N2 subtype. The second group was vaccinated at 14 days of age using the same
vaccine. The control group, received no vaccination. A number of24 birds in each group were randomly chosen
and bled via brachialis vein at day 14, 21 and 28 post vaccination, respectively. The antibody titer in the sera
was measured using hemaglutination inhibition (HI) test with homologous antigen H5N2 subtype. Data were
analysed using a split-plot design of analysis of variance of 0,05, significance level. The titer induced by vaccination
at day-7 were 1,54; 15,92 and 6,58 HI unit, respectively. Meanwhile in the second group the titer obtained were
1,29; 7,38 and 15 HI unit respectively. Antibody of the control group was negativ. Further analysis indicated that
there was significance different antibody titer induced by vaccination between treatment groups, but when
compared between group of treatment, the result showed po significance different.
Keys words: avian influenza. broiler chicken, heterologous vaccine, and antibody titer
using heterologous avian influenza H5N2 subtype at day seven and 14 in broiler chicken. A number of 120
broiler chickens was divided into three groups, each consisted of 40 birds and kept since day old chicken in
standard maintenance facilities. Group one as the treatment group was vaccinated at 7 days of age using
heterologous vaccine H5N2 subtype. The second group was vaccinated at 14 days of age using the same
vaccine. The control group, received no vaccination. A number of24 birds in each group were randomly chosen
and bled via brachialis vein at day 14, 21 and 28 post vaccination, respectively. The antibody titer in the sera
was measured using hemaglutination inhibition (HI) test with homologous antigen H5N2 subtype. Data were
analysed using a split-plot design of analysis of variance of 0,05, significance level. The titer induced by vaccination
at day-7 were 1,54; 15,92 and 6,58 HI unit, respectively. Meanwhile in the second group the titer obtained were
1,29; 7,38 and 15 HI unit respectively. Antibody of the control group was negativ. Further analysis indicated that
there was significance different antibody titer induced by vaccination between treatment groups, but when
compared between group of treatment, the result showed po significance different.
Keys words: avian influenza. broiler chicken, heterologous vaccine, and antibody titer
DOI: https://doi.org/10.22146/jsv.327
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